in-vitro phagocytosis and intracellular killing of pasteurella multocida b:2 by phagocytic cells of buffaloes
Pasteurella multocida B:2 is a Gram-negative organism causing haemorrhagic septicaemia (HS) in buffaloes. It causes severe pulmonary infection, leading to infiltration of numerous macrophages and neutrophils. Despite the inflammatory response, buffaloes succumb to HS. This study aims to evaluate the...
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| Format: | Article |
| Language: | English |
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Academic Press
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80329/1/in-vitro%20phagocytosis%20and%20intracellular%20killing%20of%20pasteurella%20multocida%20b2%20by%20phagocytic%20cells%20of%20buffaloes.pdf |
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| author | Puspitasari, Yulianna Salleh, Annas M. N., Adza Rina Saad, Mohd Zamri |
| author_facet | Puspitasari, Yulianna Salleh, Annas M. N., Adza Rina Saad, Mohd Zamri |
| author_sort | Puspitasari, Yulianna |
| collection | UPM |
| description | Pasteurella multocida B:2 is a Gram-negative organism causing haemorrhagic septicaemia (HS) in buffaloes. It causes severe pulmonary infection, leading to infiltration of numerous macrophages and neutrophils. Despite the inflammatory response, buffaloes succumb to HS. This study aims to evaluate the in-vitro efficacy of macrophages and neutrophils of buffalo following exposure to P. multocida B:2. In-vitro infections were done using 10 ⁷ cfu/ml of P. multocida B:2 for Group 1, Escherichia coli for Group 2 and Mannhaemia haemolytica A:2 for Group 3 cells. The inoculated cell cultures were harvested at 0, 30, 60 and 120 min post-exposure and the phagocytic, killing and cell death rates were determined. Both phagocytosis and killing rates of all bacteria increased over time. Phagocytosis involved between 71% and 73% neutrophils and between 60% and 64% macrophages at 120 min. Killing rate of all bacteria involved between 76% and 79% for neutrophils and between 70% and 74% for macrophages at 120 min. Death rate of neutrophils ranged between 67% in Group 3, and 88% in Group 1 at 120 min, significantly (p < 0.05) higher than Group 3 but insignificant (p > 0.05) than Group 2. Similar pattern was observed for death rate of macrophages. The phagocytosis and killing rates of P. multocida B:2 were similar to other bacterial species used in this study but more neutrophils and macrophages were dead following infection by P. multocida B:2 than M. haemolytica A:2. |
| first_indexed | 2024-03-06T10:27:53Z |
| format | Article |
| id | upm.eprints-80329 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:27:53Z |
| publishDate | 2019 |
| publisher | Academic Press |
| record_format | dspace |
| spelling | upm.eprints-803292020-10-21T19:24:09Z http://psasir.upm.edu.my/id/eprint/80329/ in-vitro phagocytosis and intracellular killing of pasteurella multocida b:2 by phagocytic cells of buffaloes Puspitasari, Yulianna Salleh, Annas M. N., Adza Rina Saad, Mohd Zamri Pasteurella multocida B:2 is a Gram-negative organism causing haemorrhagic septicaemia (HS) in buffaloes. It causes severe pulmonary infection, leading to infiltration of numerous macrophages and neutrophils. Despite the inflammatory response, buffaloes succumb to HS. This study aims to evaluate the in-vitro efficacy of macrophages and neutrophils of buffalo following exposure to P. multocida B:2. In-vitro infections were done using 10 ⁷ cfu/ml of P. multocida B:2 for Group 1, Escherichia coli for Group 2 and Mannhaemia haemolytica A:2 for Group 3 cells. The inoculated cell cultures were harvested at 0, 30, 60 and 120 min post-exposure and the phagocytic, killing and cell death rates were determined. Both phagocytosis and killing rates of all bacteria increased over time. Phagocytosis involved between 71% and 73% neutrophils and between 60% and 64% macrophages at 120 min. Killing rate of all bacteria involved between 76% and 79% for neutrophils and between 70% and 74% for macrophages at 120 min. Death rate of neutrophils ranged between 67% in Group 3, and 88% in Group 1 at 120 min, significantly (p < 0.05) higher than Group 3 but insignificant (p > 0.05) than Group 2. Similar pattern was observed for death rate of macrophages. The phagocytosis and killing rates of P. multocida B:2 were similar to other bacterial species used in this study but more neutrophils and macrophages were dead following infection by P. multocida B:2 than M. haemolytica A:2. Academic Press 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80329/1/in-vitro%20phagocytosis%20and%20intracellular%20killing%20of%20pasteurella%20multocida%20b2%20by%20phagocytic%20cells%20of%20buffaloes.pdf Puspitasari, Yulianna and Salleh, Annas and M. N., Adza Rina and Saad, Mohd Zamri (2019) in-vitro phagocytosis and intracellular killing of pasteurella multocida b:2 by phagocytic cells of buffaloes. Microbial Pathogenesis, 131. pp. 170-174. ISSN 1096-1208; ESSN: 0882-4010 https://pubmed.ncbi.nlm.nih.gov/30978429/ 10.1016/j.micpath.2019.04.012 |
| spellingShingle | Puspitasari, Yulianna Salleh, Annas M. N., Adza Rina Saad, Mohd Zamri in-vitro phagocytosis and intracellular killing of pasteurella multocida b:2 by phagocytic cells of buffaloes |
| title | in-vitro phagocytosis and intracellular killing of pasteurella multocida b:2 by phagocytic cells of buffaloes |
| title_full | in-vitro phagocytosis and intracellular killing of pasteurella multocida b:2 by phagocytic cells of buffaloes |
| title_fullStr | in-vitro phagocytosis and intracellular killing of pasteurella multocida b:2 by phagocytic cells of buffaloes |
| title_full_unstemmed | in-vitro phagocytosis and intracellular killing of pasteurella multocida b:2 by phagocytic cells of buffaloes |
| title_short | in-vitro phagocytosis and intracellular killing of pasteurella multocida b:2 by phagocytic cells of buffaloes |
| title_sort | in vitro phagocytosis and intracellular killing of pasteurella multocida b 2 by phagocytic cells of buffaloes |
| url | http://psasir.upm.edu.my/id/eprint/80329/1/in-vitro%20phagocytosis%20and%20intracellular%20killing%20of%20pasteurella%20multocida%20b2%20by%20phagocytic%20cells%20of%20buffaloes.pdf |
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