Analysis of recurrent parent genome recovery in marker-assisted backcross breeding program in watermelon
Marker–assisted backcross (MABC) is a breeding technique used to develop improved varieties by transferring a gene or QTL into the genome background of an elite variety after 2-3 generations. It is an advanced way of overcoming challenges facing conventional backcross methods as it speeds up the rec...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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IJSTR Research Publications
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80639/1/MARKER.pdf |
| _version_ | 1796980794295582720 |
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| author | Olalekan, Kazeem Kolapo Yusop, Mohd Rafii Salleh, Azrul M. TM Mohamed, Mahmud Ahmad, Khairulmazmi Misran, Azizah Abro, Tanweer F. Oladosu, Yusuff Arolu, Ibrahim Wasiu Samuel, Chukwu Usman, Magaji G. |
| author_facet | Olalekan, Kazeem Kolapo Yusop, Mohd Rafii Salleh, Azrul M. TM Mohamed, Mahmud Ahmad, Khairulmazmi Misran, Azizah Abro, Tanweer F. Oladosu, Yusuff Arolu, Ibrahim Wasiu Samuel, Chukwu Usman, Magaji G. |
| author_sort | Olalekan, Kazeem Kolapo |
| collection | UPM |
| description | Marker–assisted backcross (MABC) is a breeding technique used to develop improved varieties by transferring a gene or QTL into the genome background of an elite variety after 2-3 generations. It is an advanced way of overcoming challenges facing conventional backcross methods as it speeds up the recurrent parent genome recovery (RPG). In order to develop a Fusarium wilt resistant watermelon variety, MABC was used to incorporate wilt resistant gene from the resistant inbred line CS-19 into the genome of the high yielding but wilt susceptible inbred line BL-14. There was estimation of RPG recovery in earlier generations with the use of polymorphic simple sequence repeat (SSR) markers. A total of 380 SSR markers were tested to identify polymorphism between the parents and 78 of them were found to be polymorphic. Background analysis revealed 74.7 – 94.4 and 86.6 – 96.8 % recovery in BC1F1 and BC2F1 generations, respectively. In the BC2F2 generation, RPG recovery ranged from 95.1 and 96.9 and the average
in the selected lines was 96.14 %. This study led to the selection of plants that are similar to the recurrent parent and it showed the usefulness of MABC for the quick recovery of a parental genome in a backcrossing population. |
| first_indexed | 2024-03-06T10:28:17Z |
| format | Article |
| id | upm.eprints-80639 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:28:17Z |
| publishDate | 2019 |
| publisher | IJSTR Research Publications |
| record_format | dspace |
| spelling | upm.eprints-806392020-11-04T19:22:42Z http://psasir.upm.edu.my/id/eprint/80639/ Analysis of recurrent parent genome recovery in marker-assisted backcross breeding program in watermelon Olalekan, Kazeem Kolapo Yusop, Mohd Rafii Salleh, Azrul M. TM Mohamed, Mahmud Ahmad, Khairulmazmi Misran, Azizah Abro, Tanweer F. Oladosu, Yusuff Arolu, Ibrahim Wasiu Samuel, Chukwu Usman, Magaji G. Marker–assisted backcross (MABC) is a breeding technique used to develop improved varieties by transferring a gene or QTL into the genome background of an elite variety after 2-3 generations. It is an advanced way of overcoming challenges facing conventional backcross methods as it speeds up the recurrent parent genome recovery (RPG). In order to develop a Fusarium wilt resistant watermelon variety, MABC was used to incorporate wilt resistant gene from the resistant inbred line CS-19 into the genome of the high yielding but wilt susceptible inbred line BL-14. There was estimation of RPG recovery in earlier generations with the use of polymorphic simple sequence repeat (SSR) markers. A total of 380 SSR markers were tested to identify polymorphism between the parents and 78 of them were found to be polymorphic. Background analysis revealed 74.7 – 94.4 and 86.6 – 96.8 % recovery in BC1F1 and BC2F1 generations, respectively. In the BC2F2 generation, RPG recovery ranged from 95.1 and 96.9 and the average in the selected lines was 96.14 %. This study led to the selection of plants that are similar to the recurrent parent and it showed the usefulness of MABC for the quick recovery of a parental genome in a backcrossing population. IJSTR Research Publications 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80639/1/MARKER.pdf Olalekan, Kazeem Kolapo and Yusop, Mohd Rafii and Salleh, Azrul M. and TM Mohamed, Mahmud and Ahmad, Khairulmazmi and Misran, Azizah and Abro, Tanweer F. and Oladosu, Yusuff and Arolu, Ibrahim Wasiu and Samuel, Chukwu and Usman, Magaji G. (2019) Analysis of recurrent parent genome recovery in marker-assisted backcross breeding program in watermelon. International Journal of Scientific and Technology Research, 8 (8). pp. 945-955. ISSN 2277-8616 https://www.ijstr.org/final-print/aug2019/Analysis-Of-Recurrent-Parent-Genome-Recovery-In-Marker-assisted-Backcross-Breeding-Program-In-Watermelon.pdf |
| spellingShingle | Olalekan, Kazeem Kolapo Yusop, Mohd Rafii Salleh, Azrul M. TM Mohamed, Mahmud Ahmad, Khairulmazmi Misran, Azizah Abro, Tanweer F. Oladosu, Yusuff Arolu, Ibrahim Wasiu Samuel, Chukwu Usman, Magaji G. Analysis of recurrent parent genome recovery in marker-assisted backcross breeding program in watermelon |
| title | Analysis of recurrent parent genome recovery in marker-assisted backcross breeding program in watermelon |
| title_full | Analysis of recurrent parent genome recovery in marker-assisted backcross breeding program in watermelon |
| title_fullStr | Analysis of recurrent parent genome recovery in marker-assisted backcross breeding program in watermelon |
| title_full_unstemmed | Analysis of recurrent parent genome recovery in marker-assisted backcross breeding program in watermelon |
| title_short | Analysis of recurrent parent genome recovery in marker-assisted backcross breeding program in watermelon |
| title_sort | analysis of recurrent parent genome recovery in marker assisted backcross breeding program in watermelon |
| url | http://psasir.upm.edu.my/id/eprint/80639/1/MARKER.pdf |
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