Mass transfer with reaction kinetics of the biocatalytic membrane reactor using a fouled covalently immobilised enzyme layer (α–CGTase–CNF layer)
A dynamic mathematical model was used in this study to describe the mass transfer and reaction kinetics of a fouled α–CGTase–CNF layer in a biocatalytic membrane reactor (BMR) system. BMR performance was evaluated based on the effect of substrate concentration and pneumatic gauge pressure on the per...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81290/1/Mass%20transfer%20with%20reaction%20kinetics%20.pdf |
| _version_ | 1796980889602752512 |
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| author | Sulaiman, Safwan Mokhtar, Mohd Noriznan Mohd Nor, Mohd Zuhair Md. Yunos, Khairul Faezah Naim, Mohd Nazli |
| author_facet | Sulaiman, Safwan Mokhtar, Mohd Noriznan Mohd Nor, Mohd Zuhair Md. Yunos, Khairul Faezah Naim, Mohd Nazli |
| author_sort | Sulaiman, Safwan |
| collection | UPM |
| description | A dynamic mathematical model was used in this study to describe the mass transfer and reaction kinetics of a fouled α–CGTase–CNF layer in a biocatalytic membrane reactor (BMR) system. BMR performance was evaluated based on the effect of substrate concentration and pneumatic gauge pressure on the permeate flux, total permeate volume, starch conversion, and α-CD production. A model is introduced based on the layer of mass limit as the resistance blocking mechanism with reaction kinetics on the fouled α–CGTase–CNF layer. Important unknown parameters of the constants describing the layer resistance, KML0 and the kinetic constant of Michaelis-Menten, kcat, for mass transfer and its reaction kinetics, were successfully estimated at 3.9 × 107 min/mL and 216.18 mg-starch/cm3⋅U⋅min, respectively, using the proposed models. The study found that the kcat value corresponding to the maximum efficiency of the enzyme on starch cyclisation was a critical parameter in the prediction of the BMR dynamic profiles. Thus, the proposed model in this study can be used for further development of the BMR system, with excellent hydrodynamic and reaction performance. |
| first_indexed | 2024-03-06T10:29:41Z |
| format | Article |
| id | upm.eprints-81290 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:29:41Z |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-812902021-10-17T21:59:39Z http://psasir.upm.edu.my/id/eprint/81290/ Mass transfer with reaction kinetics of the biocatalytic membrane reactor using a fouled covalently immobilised enzyme layer (α–CGTase–CNF layer) Sulaiman, Safwan Mokhtar, Mohd Noriznan Mohd Nor, Mohd Zuhair Md. Yunos, Khairul Faezah Naim, Mohd Nazli A dynamic mathematical model was used in this study to describe the mass transfer and reaction kinetics of a fouled α–CGTase–CNF layer in a biocatalytic membrane reactor (BMR) system. BMR performance was evaluated based on the effect of substrate concentration and pneumatic gauge pressure on the permeate flux, total permeate volume, starch conversion, and α-CD production. A model is introduced based on the layer of mass limit as the resistance blocking mechanism with reaction kinetics on the fouled α–CGTase–CNF layer. Important unknown parameters of the constants describing the layer resistance, KML0 and the kinetic constant of Michaelis-Menten, kcat, for mass transfer and its reaction kinetics, were successfully estimated at 3.9 × 107 min/mL and 216.18 mg-starch/cm3⋅U⋅min, respectively, using the proposed models. The study found that the kcat value corresponding to the maximum efficiency of the enzyme on starch cyclisation was a critical parameter in the prediction of the BMR dynamic profiles. Thus, the proposed model in this study can be used for further development of the BMR system, with excellent hydrodynamic and reaction performance. Elsevier 2019-12-15 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81290/1/Mass%20transfer%20with%20reaction%20kinetics%20.pdf Sulaiman, Safwan and Mokhtar, Mohd Noriznan and Mohd Nor, Mohd Zuhair and Md. Yunos, Khairul Faezah and Naim, Mohd Nazli (2019) Mass transfer with reaction kinetics of the biocatalytic membrane reactor using a fouled covalently immobilised enzyme layer (α–CGTase–CNF layer). Biochemical Engineering Journal, 152. art. no. 107374. ISSN 1369-703X https://www.sciencedirect.com/science/article/abs/pii/S1369703X19303134 10.1016/j.bej.2019.107374 |
| spellingShingle | Sulaiman, Safwan Mokhtar, Mohd Noriznan Mohd Nor, Mohd Zuhair Md. Yunos, Khairul Faezah Naim, Mohd Nazli Mass transfer with reaction kinetics of the biocatalytic membrane reactor using a fouled covalently immobilised enzyme layer (α–CGTase–CNF layer) |
| title | Mass transfer with reaction kinetics of the biocatalytic membrane reactor using a fouled covalently immobilised enzyme layer (α–CGTase–CNF layer) |
| title_full | Mass transfer with reaction kinetics of the biocatalytic membrane reactor using a fouled covalently immobilised enzyme layer (α–CGTase–CNF layer) |
| title_fullStr | Mass transfer with reaction kinetics of the biocatalytic membrane reactor using a fouled covalently immobilised enzyme layer (α–CGTase–CNF layer) |
| title_full_unstemmed | Mass transfer with reaction kinetics of the biocatalytic membrane reactor using a fouled covalently immobilised enzyme layer (α–CGTase–CNF layer) |
| title_short | Mass transfer with reaction kinetics of the biocatalytic membrane reactor using a fouled covalently immobilised enzyme layer (α–CGTase–CNF layer) |
| title_sort | mass transfer with reaction kinetics of the biocatalytic membrane reactor using a fouled covalently immobilised enzyme layer α cgtase cnf layer |
| url | http://psasir.upm.edu.my/id/eprint/81290/1/Mass%20transfer%20with%20reaction%20kinetics%20.pdf |
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