Mode of action of Jatropha curcas phorbol esters in bovine kidney cells
Jatropha meal is a potential biofeed for animal production however, the presence of phorbol esters (PEs) in the meal limits its utilization. The PEs caused severe toxic manifestations in animals. The information on the mechanisms of toxicity at the cellular level is rather limited. Therefore, this r...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81376/1/Mode%20of%20action%20of%20Jatropha%20curcas%20phorbol%20esters%20in%20bovine%20kidney%20cells.pdf |
| _version_ | 1796980904219901952 |
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| author | Oskoueian, Ehsan Abdullah, Norhani Noura, Reza Ebrahimi, Mahdi Ahmad, Syahida Shakeri, Majid |
| author_facet | Oskoueian, Ehsan Abdullah, Norhani Noura, Reza Ebrahimi, Mahdi Ahmad, Syahida Shakeri, Majid |
| author_sort | Oskoueian, Ehsan |
| collection | UPM |
| description | Jatropha meal is a potential biofeed for animal production however, the presence of phorbol esters (PEs) in the meal limits its utilization. The PEs caused severe toxic manifestations in animals. The information on the mechanisms of toxicity at the cellular level is rather limited. Therefore, this research was conducted to investigate the mode of action of PEs by evaluating the biochemical, biological and molecular responses of cells exposed to PEs. Phorbol esters from Jatropha meal were isolated and identified as Jatropha factor (JF) JFC1, JFC2, JFC3 and a mixture of JFs (C4, C5 and C6). These PEs exhibited cytotoxic activity towards bovine kidney cells and induced significant morphological changes. The PEs severely altered the redox status of the cells which resulted in the occurrence of oxidative stress. Moreover, the PEs up-regulated the expressions of PKC-βII, proto-oncogenes, pro-inflammatory cytokines and elevated the caspase-3 activity in the cells. All PEs were toxic, but JFC1 and JFC2 appeared to be more toxic to the bovine kidney cells as compared to the JFC3 and the mixture of JFs. The results indicated that the cytotoxic activity of Jatropha meal PEs was elicited through induction of oxidative stress and inflammation of the cells. |
| first_indexed | 2024-03-06T10:29:54Z |
| format | Article |
| id | upm.eprints-81376 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:29:54Z |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-813762021-02-23T17:54:22Z http://psasir.upm.edu.my/id/eprint/81376/ Mode of action of Jatropha curcas phorbol esters in bovine kidney cells Oskoueian, Ehsan Abdullah, Norhani Noura, Reza Ebrahimi, Mahdi Ahmad, Syahida Shakeri, Majid Jatropha meal is a potential biofeed for animal production however, the presence of phorbol esters (PEs) in the meal limits its utilization. The PEs caused severe toxic manifestations in animals. The information on the mechanisms of toxicity at the cellular level is rather limited. Therefore, this research was conducted to investigate the mode of action of PEs by evaluating the biochemical, biological and molecular responses of cells exposed to PEs. Phorbol esters from Jatropha meal were isolated and identified as Jatropha factor (JF) JFC1, JFC2, JFC3 and a mixture of JFs (C4, C5 and C6). These PEs exhibited cytotoxic activity towards bovine kidney cells and induced significant morphological changes. The PEs severely altered the redox status of the cells which resulted in the occurrence of oxidative stress. Moreover, the PEs up-regulated the expressions of PKC-βII, proto-oncogenes, pro-inflammatory cytokines and elevated the caspase-3 activity in the cells. All PEs were toxic, but JFC1 and JFC2 appeared to be more toxic to the bovine kidney cells as compared to the JFC3 and the mixture of JFs. The results indicated that the cytotoxic activity of Jatropha meal PEs was elicited through induction of oxidative stress and inflammation of the cells. Elsevier 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81376/1/Mode%20of%20action%20of%20Jatropha%20curcas%20phorbol%20esters%20in%20bovine%20kidney%20cells.pdf Oskoueian, Ehsan and Abdullah, Norhani and Noura, Reza and Ebrahimi, Mahdi and Ahmad, Syahida and Shakeri, Majid (2019) Mode of action of Jatropha curcas phorbol esters in bovine kidney cells. Biocatalysis and Agricultural Biotechnology, 17. pp. 514-524. ISSN 1878-8181 https://www.sciencedirect.com/science/article/abs/pii/S187881811830608X 10.1016/j.bcab.2019.01.010 |
| spellingShingle | Oskoueian, Ehsan Abdullah, Norhani Noura, Reza Ebrahimi, Mahdi Ahmad, Syahida Shakeri, Majid Mode of action of Jatropha curcas phorbol esters in bovine kidney cells |
| title | Mode of action of Jatropha curcas phorbol esters in bovine kidney cells |
| title_full | Mode of action of Jatropha curcas phorbol esters in bovine kidney cells |
| title_fullStr | Mode of action of Jatropha curcas phorbol esters in bovine kidney cells |
| title_full_unstemmed | Mode of action of Jatropha curcas phorbol esters in bovine kidney cells |
| title_short | Mode of action of Jatropha curcas phorbol esters in bovine kidney cells |
| title_sort | mode of action of jatropha curcas phorbol esters in bovine kidney cells |
| url | http://psasir.upm.edu.my/id/eprint/81376/1/Mode%20of%20action%20of%20Jatropha%20curcas%20phorbol%20esters%20in%20bovine%20kidney%20cells.pdf |
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