Molecular epidemiology of Sporothrix schenkii isolates in Malaysia
Sporothrix schenkii is a dimorphic fungus that causes infections in both humans and animals. We report on 25 S. schenkii isolates collected in 2017 from humans and cats clinically diagnosed with sporotrichosis, in Malaysia. These isolates were phenotypically identified as S. schenkii sensu lato and...
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| Format: | Article |
| Language: | English |
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Oxford University Press
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81412/1/Molecular%20epidemiology%20of%20Sporothrix%20schenkii%20isolates%20in%20Malaysia.pdf |
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| author | Kamal Azam, Nurul Khalisah Selvarajah, Gayathri Thevi Santhanam, Jacinta Abd Razak, Mohd Fuat Ginsapu, Stephanie Jane James, Jasper Elvin Suetrong, Satinee |
| author_facet | Kamal Azam, Nurul Khalisah Selvarajah, Gayathri Thevi Santhanam, Jacinta Abd Razak, Mohd Fuat Ginsapu, Stephanie Jane James, Jasper Elvin Suetrong, Satinee |
| author_sort | Kamal Azam, Nurul Khalisah |
| collection | UPM |
| description | Sporothrix schenkii is a dimorphic fungus that causes infections in both humans and animals. We report on 25 S. schenkii isolates collected in 2017 from humans and cats clinically diagnosed with sporotrichosis, in Malaysia. These isolates were phenotypically identified as S. schenkii sensu lato and further defined as S. schenckii sensu stricto based on partial calmodulin gene sequence. Isolates from both humans and cats were genotypically identical but displayed phenotypic variation. Phylogenetic analyses based on partial calmodulin sequence showed that the Malaysian isolates clustered with global S. schenkii sensu stricto strains, in particular, of the AFLP type E. This analysis also revealed that partial calmodulin sequence alone was sufficient for classifying global S. schenckii sensu stricto strains into their respective AFLP types, from A to E. The genetically conserved S. schenkii sensu stricto species isolated from humans and cats is suggestive of a clonal strain present in Malaysia. To the best of our knowledge, this is the first report on molecular identification of Sporothrix schenkii strains from human infections in Malaysia. Further studies are required in order to elucidate the clonal nature of Malaysian S. schenkii isolates. Our findings indicate the presence of a predominant S. schenkii genotype in the environment, causing infections in both cats and humans in Malaysia. |
| first_indexed | 2024-03-06T10:30:00Z |
| format | Article |
| id | upm.eprints-81412 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:30:00Z |
| publishDate | 2019 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | upm.eprints-814122021-02-13T19:15:40Z http://psasir.upm.edu.my/id/eprint/81412/ Molecular epidemiology of Sporothrix schenkii isolates in Malaysia Kamal Azam, Nurul Khalisah Selvarajah, Gayathri Thevi Santhanam, Jacinta Abd Razak, Mohd Fuat Ginsapu, Stephanie Jane James, Jasper Elvin Suetrong, Satinee Sporothrix schenkii is a dimorphic fungus that causes infections in both humans and animals. We report on 25 S. schenkii isolates collected in 2017 from humans and cats clinically diagnosed with sporotrichosis, in Malaysia. These isolates were phenotypically identified as S. schenkii sensu lato and further defined as S. schenckii sensu stricto based on partial calmodulin gene sequence. Isolates from both humans and cats were genotypically identical but displayed phenotypic variation. Phylogenetic analyses based on partial calmodulin sequence showed that the Malaysian isolates clustered with global S. schenkii sensu stricto strains, in particular, of the AFLP type E. This analysis also revealed that partial calmodulin sequence alone was sufficient for classifying global S. schenckii sensu stricto strains into their respective AFLP types, from A to E. The genetically conserved S. schenkii sensu stricto species isolated from humans and cats is suggestive of a clonal strain present in Malaysia. To the best of our knowledge, this is the first report on molecular identification of Sporothrix schenkii strains from human infections in Malaysia. Further studies are required in order to elucidate the clonal nature of Malaysian S. schenkii isolates. Our findings indicate the presence of a predominant S. schenkii genotype in the environment, causing infections in both cats and humans in Malaysia. Oxford University Press 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81412/1/Molecular%20epidemiology%20of%20Sporothrix%20schenkii%20isolates%20in%20Malaysia.pdf Kamal Azam, Nurul Khalisah and Selvarajah, Gayathri Thevi and Santhanam, Jacinta and Abd Razak, Mohd Fuat and Ginsapu, Stephanie Jane and James, Jasper Elvin and Suetrong, Satinee (2019) Molecular epidemiology of Sporothrix schenkii isolates in Malaysia. Medical Mycology, 58 (5). pp. 617-625. ISSN 1369-3786; ESSN: 1460-2709 https://academic.oup.com/mmy/article-abstract/58/5/617/5603202?redirectedFrom=fulltext 10.1093/mmy/myz106 |
| spellingShingle | Kamal Azam, Nurul Khalisah Selvarajah, Gayathri Thevi Santhanam, Jacinta Abd Razak, Mohd Fuat Ginsapu, Stephanie Jane James, Jasper Elvin Suetrong, Satinee Molecular epidemiology of Sporothrix schenkii isolates in Malaysia |
| title | Molecular epidemiology of Sporothrix schenkii isolates in Malaysia |
| title_full | Molecular epidemiology of Sporothrix schenkii isolates in Malaysia |
| title_fullStr | Molecular epidemiology of Sporothrix schenkii isolates in Malaysia |
| title_full_unstemmed | Molecular epidemiology of Sporothrix schenkii isolates in Malaysia |
| title_short | Molecular epidemiology of Sporothrix schenkii isolates in Malaysia |
| title_sort | molecular epidemiology of sporothrix schenkii isolates in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/81412/1/Molecular%20epidemiology%20of%20Sporothrix%20schenkii%20isolates%20in%20Malaysia.pdf |
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