Optimization of culture conditions of soymilk for equol production by Bifidobacterium breve 15700 and Bifidobacterium longum BB536
This study analyzed the effect of pH (X1), temperature (X2) and inulin amount (X3) on transformation of isoflavones (daidzin and daidzein) to equol in soymilk fermented with Bifidobacterium spp. All responses significantly (p < 0.05) fitted into quadratic models with coefficients of determination...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81565/1/Optimization%20of%20culture%20conditions%20of%20soymilk%20for%20equol%20production%20by%20Bifidobacterium%20breve%2015700%20and%20Bifidobacterium%20longum%20BB536.pdf |
| _version_ | 1825951461593317376 |
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| author | Mustafa, Salma Elghali Mustafa, Shuhaimi Abas, Faridah Abd Manap, Mohd Yazid Ismail, Amin Amid, Mehrnoush Elzen, Salma |
| author_facet | Mustafa, Salma Elghali Mustafa, Shuhaimi Abas, Faridah Abd Manap, Mohd Yazid Ismail, Amin Amid, Mehrnoush Elzen, Salma |
| author_sort | Mustafa, Salma Elghali |
| collection | UPM |
| description | This study analyzed the effect of pH (X1), temperature (X2) and inulin amount (X3) on transformation of isoflavones (daidzin and daidzein) to equol in soymilk fermented with Bifidobacterium spp. All responses significantly (p < 0.05) fitted into quadratic models with coefficients of determination (R2) close to 1 (0.935–0.989). At 24 h of fermentation, amounts of daidzin and daidzein were influenced by all factors. While at 48 h, all factors affected daidzin and only temperature affected daidzein. Equol production was influenced by pH and temperature in 24 h and by all factors in 48 h fermentation. The optimum conditions for equol production were pH 8, 30 °C and 0.5% inulin. Model validation demonstrated there was no significant (p > 0.05) difference between the experimental and predicted values, suggested the suitability of established models in explaining the daidzin and daidzein transformation to equol as a function of pH, temperature and inulin. |
| first_indexed | 2024-03-06T10:30:22Z |
| format | Article |
| id | upm.eprints-81565 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:30:22Z |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-815652021-01-28T18:09:43Z http://psasir.upm.edu.my/id/eprint/81565/ Optimization of culture conditions of soymilk for equol production by Bifidobacterium breve 15700 and Bifidobacterium longum BB536 Mustafa, Salma Elghali Mustafa, Shuhaimi Abas, Faridah Abd Manap, Mohd Yazid Ismail, Amin Amid, Mehrnoush Elzen, Salma This study analyzed the effect of pH (X1), temperature (X2) and inulin amount (X3) on transformation of isoflavones (daidzin and daidzein) to equol in soymilk fermented with Bifidobacterium spp. All responses significantly (p < 0.05) fitted into quadratic models with coefficients of determination (R2) close to 1 (0.935–0.989). At 24 h of fermentation, amounts of daidzin and daidzein were influenced by all factors. While at 48 h, all factors affected daidzin and only temperature affected daidzein. Equol production was influenced by pH and temperature in 24 h and by all factors in 48 h fermentation. The optimum conditions for equol production were pH 8, 30 °C and 0.5% inulin. Model validation demonstrated there was no significant (p > 0.05) difference between the experimental and predicted values, suggested the suitability of established models in explaining the daidzin and daidzein transformation to equol as a function of pH, temperature and inulin. Elsevier 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81565/1/Optimization%20of%20culture%20conditions%20of%20soymilk%20for%20equol%20production%20by%20Bifidobacterium%20breve%2015700%20and%20Bifidobacterium%20longum%20BB536.pdf Mustafa, Salma Elghali and Mustafa, Shuhaimi and Abas, Faridah and Abd Manap, Mohd Yazid and Ismail, Amin and Amid, Mehrnoush and Elzen, Salma (2019) Optimization of culture conditions of soymilk for equol production by Bifidobacterium breve 15700 and Bifidobacterium longum BB536. Food Chemistry, 278. pp. 767-772. ISSN 0308-8146 https://www.sciencedirect.com/science/article/abs/pii/S0308814618320521 10.1016/j.foodchem.2018.11.107 |
| spellingShingle | Mustafa, Salma Elghali Mustafa, Shuhaimi Abas, Faridah Abd Manap, Mohd Yazid Ismail, Amin Amid, Mehrnoush Elzen, Salma Optimization of culture conditions of soymilk for equol production by Bifidobacterium breve 15700 and Bifidobacterium longum BB536 |
| title | Optimization of culture conditions of soymilk for equol production by Bifidobacterium breve 15700 and Bifidobacterium longum BB536 |
| title_full | Optimization of culture conditions of soymilk for equol production by Bifidobacterium breve 15700 and Bifidobacterium longum BB536 |
| title_fullStr | Optimization of culture conditions of soymilk for equol production by Bifidobacterium breve 15700 and Bifidobacterium longum BB536 |
| title_full_unstemmed | Optimization of culture conditions of soymilk for equol production by Bifidobacterium breve 15700 and Bifidobacterium longum BB536 |
| title_short | Optimization of culture conditions of soymilk for equol production by Bifidobacterium breve 15700 and Bifidobacterium longum BB536 |
| title_sort | optimization of culture conditions of soymilk for equol production by bifidobacterium breve 15700 and bifidobacterium longum bb536 |
| url | http://psasir.upm.edu.my/id/eprint/81565/1/Optimization%20of%20culture%20conditions%20of%20soymilk%20for%20equol%20production%20by%20Bifidobacterium%20breve%2015700%20and%20Bifidobacterium%20longum%20BB536.pdf |
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