Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows
Mastitis is the inflammation of the mammary gland due to microbial infiltration causing a reduced mammary function. This study aims at developing a vaccine using Malaysian local isolate of Staphylococcus aureus and evaluating serum amyloid A, Interleukin-10, IgM and IgG responses periodically. Four...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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Springer Nature
2019
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| _version_ | 1825951477713076224 |
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| author | Hambali, Idris Umar Abdullah, Faez Firdaus Jesse Bhutto, K. R. Mohd Azmi, M. L. Wahid, A. H. Zakaria, Z. Odhah, M. N. Arsalan, M. Muhammad, N. A. Jefri, M. N. |
| author_facet | Hambali, Idris Umar Abdullah, Faez Firdaus Jesse Bhutto, K. R. Mohd Azmi, M. L. Wahid, A. H. Zakaria, Z. Odhah, M. N. Arsalan, M. Muhammad, N. A. Jefri, M. N. |
| author_sort | Hambali, Idris Umar |
| collection | UPM |
| description | Mastitis is the inflammation of the mammary gland due to microbial infiltration causing a reduced mammary function. This study aims at developing a vaccine using Malaysian local isolate of Staphylococcus aureus and evaluating serum amyloid A, Interleukin-10, IgM and IgG responses periodically. Four bacterin concentrations (10⁶, 10⁷, 10⁸ and 10⁹ cfu/ml of the local isolate of S. aureus) were adjuvanted with aluminium potassium sulphate. Thirty cows grouped into 4 treatment groups (G-) were vaccinated (2 ml) intramuscularly, with a fifth G-A as control. The mean concentration (MC) of serum amyloid A (SAA) was significantly different (sig-d) (p ˂ 0.05) in G-D at 0 h post vaccination (PV), 3 h PV, 24 h PV, weeks 1, 2, 3 and 4 PV (6-, 15-, 5-, 12-, 11-, 4- and 11-fold increased (FI) respectively). The MC of serum amyloid A was also sig-d in G-E at 0 h PV, weeks 1, 2 and 4 PV (3, 8, 5 and 8 FI respectively). The MC of IL-10 was sig-d in G-D and C at 3 h PV and week 2 PV (5 and 2 FI respectively). The IgM MC was sig-d in G-B and C at 3 h PV (5 and 6 FI respectively), at 24 h PV (5 and 9 FI respectively), at week 3 PV(2 and 2 FI respectively) and week 4 PV (3 and 4 FI respectively). The MC of IgG was sig-d in G-E at 0 h, 3 h and week 3 PV(5, 6 and 2 FI respectively) and in G-D at weeks 1–4 (3, 3, 3 and 5 FI respectively). In conclusion, elevated levels of SAA, IgG and IL-10 in G-D(10⁸) informed our choice of best dosage which can be used to evoke immunity in cows. |
| first_indexed | 2024-09-25T03:35:17Z |
| format | Article |
| id | upm.eprints-81678 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:35:17Z |
| publishDate | 2019 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | upm.eprints-816782024-05-16T07:45:32Z http://psasir.upm.edu.my/id/eprint/81678/ Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows Hambali, Idris Umar Abdullah, Faez Firdaus Jesse Bhutto, K. R. Mohd Azmi, M. L. Wahid, A. H. Zakaria, Z. Odhah, M. N. Arsalan, M. Muhammad, N. A. Jefri, M. N. Mastitis is the inflammation of the mammary gland due to microbial infiltration causing a reduced mammary function. This study aims at developing a vaccine using Malaysian local isolate of Staphylococcus aureus and evaluating serum amyloid A, Interleukin-10, IgM and IgG responses periodically. Four bacterin concentrations (10⁶, 10⁷, 10⁸ and 10⁹ cfu/ml of the local isolate of S. aureus) were adjuvanted with aluminium potassium sulphate. Thirty cows grouped into 4 treatment groups (G-) were vaccinated (2 ml) intramuscularly, with a fifth G-A as control. The mean concentration (MC) of serum amyloid A (SAA) was significantly different (sig-d) (p ˂ 0.05) in G-D at 0 h post vaccination (PV), 3 h PV, 24 h PV, weeks 1, 2, 3 and 4 PV (6-, 15-, 5-, 12-, 11-, 4- and 11-fold increased (FI) respectively). The MC of serum amyloid A was also sig-d in G-E at 0 h PV, weeks 1, 2 and 4 PV (3, 8, 5 and 8 FI respectively). The MC of IL-10 was sig-d in G-D and C at 3 h PV and week 2 PV (5 and 2 FI respectively). The IgM MC was sig-d in G-B and C at 3 h PV (5 and 6 FI respectively), at 24 h PV (5 and 9 FI respectively), at week 3 PV(2 and 2 FI respectively) and week 4 PV (3 and 4 FI respectively). The MC of IgG was sig-d in G-E at 0 h, 3 h and week 3 PV(5, 6 and 2 FI respectively) and in G-D at weeks 1–4 (3, 3, 3 and 5 FI respectively). In conclusion, elevated levels of SAA, IgG and IL-10 in G-D(10⁸) informed our choice of best dosage which can be used to evoke immunity in cows. Springer Nature 2019 Article PeerReviewed Hambali, Idris Umar and Abdullah, Faez Firdaus Jesse and Bhutto, K. R. and Mohd Azmi, M. L. and Wahid, A. H. and Zakaria, Z. and Odhah, M. N. and Arsalan, M. and Muhammad, N. A. and Jefri, M. N. (2019) Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows. Tropical Animal Health and Production, 51. pp. 781-789. ISSN 0049-4747; ESSN: 1573-7438 https://link.springer.com/article/10.1007/s11250-018-1755-8?error=cookies_not_supported&code=266b469e-d3f7-46a1-97ab-9fed7d7f11d9 10.1007/s11250-018-1755-8 |
| spellingShingle | Hambali, Idris Umar Abdullah, Faez Firdaus Jesse Bhutto, K. R. Mohd Azmi, M. L. Wahid, A. H. Zakaria, Z. Odhah, M. N. Arsalan, M. Muhammad, N. A. Jefri, M. N. Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows |
| title | Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows |
| title_full | Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows |
| title_fullStr | Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows |
| title_full_unstemmed | Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows |
| title_short | Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows |
| title_sort | periodic vicissitudes of different concentrations of a developed prototype killed s aureus mastitis vaccine on immune modulators mediators and immunoglobulins in cows |
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