Phytochemical analysis of Elateriospermum tapos and its inhibitory effects on alpha-amylase, alpha-glucosidase and pancreatic lipase
Elateriospermum tapos contains high unsaturated fat and phytochemicals with many health benefits. This paper focuses on activities and inhibitory effects of E. tapos on digestive enzymes. Cold water, hot water and 70% ethanol extracts of the seed and shell of the fruit of E. tapos were used in thi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Forest Research Institute Malaysia
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81736/1/Phytochemical%20analysis%20of%20Elateriospermum%20tapos.pdf |
| _version_ | 1825951482964344832 |
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| author | Ja'afar, Nor Liyana K Thiagarajan, Siroshini MB, Nurul Syahirah Chang, Wei Lin Shafie, Nurul Husna Arapoc, Daryl Jesus Abdul Kadir, Khairul Kamilah Bahari, Hasnah |
| author_facet | Ja'afar, Nor Liyana K Thiagarajan, Siroshini MB, Nurul Syahirah Chang, Wei Lin Shafie, Nurul Husna Arapoc, Daryl Jesus Abdul Kadir, Khairul Kamilah Bahari, Hasnah |
| author_sort | Ja'afar, Nor Liyana |
| collection | UPM |
| description | Elateriospermum tapos contains high unsaturated fat and phytochemicals with many health benefits. This
paper focuses on activities and inhibitory effects of E. tapos on digestive enzymes. Cold water, hot water and
70% ethanol extracts of the seed and shell of the fruit of E. tapos were used in this study. The extracts were
screened for antioxidant activity, total phenolic content, total flavonoid content, and inhibitory effects on
α-amylase, α-glucosidase and pancreatic lipase. Hot water extraction of shell of the E. tapos fruit had the
highest total phenolic content (1298.60 ± 4. 24 µg GAE 100 g-1), total flavonoid content (16685.58 ± 487.77 µg
CE 100 g-1) and antioxidant activity by 2, 2-diphenyl-2-picrylhydrazyl and β-carotene methods (84.16 and
122.17% respectively). The seed cold extract showed maximum α-amylase inhibition with IC50 (half maximal
inhibitory concentration) of 0.03 mg mL-1. The lowest IC50 (0.02 mg mL-1) for α-glucosidase inhibition
was from seed ethanol extracts while shell cold extract had the lowest IC50 for pancreatic lipase inhibition
(37.80 mg mL-1). Results confirmed E. tapos as potential antioxidant and inhibitor of digestive enzymes for
lipid (pancreatic lipase) and carbohydrate (α-amylase and α-glucosidase) which are beneficial to combat
obesity and diabetes. |
| first_indexed | 2024-03-06T10:30:39Z |
| format | Article |
| id | upm.eprints-81736 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:30:39Z |
| publishDate | 2018 |
| publisher | Forest Research Institute Malaysia |
| record_format | dspace |
| spelling | upm.eprints-817362022-12-13T08:11:31Z http://psasir.upm.edu.my/id/eprint/81736/ Phytochemical analysis of Elateriospermum tapos and its inhibitory effects on alpha-amylase, alpha-glucosidase and pancreatic lipase Ja'afar, Nor Liyana K Thiagarajan, Siroshini MB, Nurul Syahirah Chang, Wei Lin Shafie, Nurul Husna Arapoc, Daryl Jesus Abdul Kadir, Khairul Kamilah Bahari, Hasnah Elateriospermum tapos contains high unsaturated fat and phytochemicals with many health benefits. This paper focuses on activities and inhibitory effects of E. tapos on digestive enzymes. Cold water, hot water and 70% ethanol extracts of the seed and shell of the fruit of E. tapos were used in this study. The extracts were screened for antioxidant activity, total phenolic content, total flavonoid content, and inhibitory effects on α-amylase, α-glucosidase and pancreatic lipase. Hot water extraction of shell of the E. tapos fruit had the highest total phenolic content (1298.60 ± 4. 24 µg GAE 100 g-1), total flavonoid content (16685.58 ± 487.77 µg CE 100 g-1) and antioxidant activity by 2, 2-diphenyl-2-picrylhydrazyl and β-carotene methods (84.16 and 122.17% respectively). The seed cold extract showed maximum α-amylase inhibition with IC50 (half maximal inhibitory concentration) of 0.03 mg mL-1. The lowest IC50 (0.02 mg mL-1) for α-glucosidase inhibition was from seed ethanol extracts while shell cold extract had the lowest IC50 for pancreatic lipase inhibition (37.80 mg mL-1). Results confirmed E. tapos as potential antioxidant and inhibitor of digestive enzymes for lipid (pancreatic lipase) and carbohydrate (α-amylase and α-glucosidase) which are beneficial to combat obesity and diabetes. Forest Research Institute Malaysia 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81736/1/Phytochemical%20analysis%20of%20Elateriospermum%20tapos.pdf Ja'afar, Nor Liyana and K Thiagarajan, Siroshini and MB, Nurul Syahirah and Chang, Wei Lin and Shafie, Nurul Husna and Arapoc, Daryl Jesus and Abdul Kadir, Khairul Kamilah and Bahari, Hasnah (2018) Phytochemical analysis of Elateriospermum tapos and its inhibitory effects on alpha-amylase, alpha-glucosidase and pancreatic lipase. Journal of Tropical Forest Science, 31 (2). pp. 240-248. ISSN 0128-1283 https://www.proquest.com/docview/2224303054?parentSessionId=xPt5OpW0k7NS%2B%2B1qzmWO7TafJ%2FmxD75aMXDWq%2BXj3io%3D 10.26525/jtfs2019.31.2.240248 |
| spellingShingle | Ja'afar, Nor Liyana K Thiagarajan, Siroshini MB, Nurul Syahirah Chang, Wei Lin Shafie, Nurul Husna Arapoc, Daryl Jesus Abdul Kadir, Khairul Kamilah Bahari, Hasnah Phytochemical analysis of Elateriospermum tapos and its inhibitory effects on alpha-amylase, alpha-glucosidase and pancreatic lipase |
| title | Phytochemical analysis of Elateriospermum tapos and its inhibitory effects on alpha-amylase, alpha-glucosidase and pancreatic lipase |
| title_full | Phytochemical analysis of Elateriospermum tapos and its inhibitory effects on alpha-amylase, alpha-glucosidase and pancreatic lipase |
| title_fullStr | Phytochemical analysis of Elateriospermum tapos and its inhibitory effects on alpha-amylase, alpha-glucosidase and pancreatic lipase |
| title_full_unstemmed | Phytochemical analysis of Elateriospermum tapos and its inhibitory effects on alpha-amylase, alpha-glucosidase and pancreatic lipase |
| title_short | Phytochemical analysis of Elateriospermum tapos and its inhibitory effects on alpha-amylase, alpha-glucosidase and pancreatic lipase |
| title_sort | phytochemical analysis of elateriospermum tapos and its inhibitory effects on alpha amylase alpha glucosidase and pancreatic lipase |
| url | http://psasir.upm.edu.my/id/eprint/81736/1/Phytochemical%20analysis%20of%20Elateriospermum%20tapos.pdf |
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