Recovery of protein from dairy milk waste product using alcohol-salt liquid biphasic flotation
Expired dairy products are often disposed of due to the potential health hazard they pose to living organisms. Lack of methods to recover valuable components from them are also a reason for manufactures to dispose of the expired dairy products. Milk encompasses several different components with thei...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81946/1/Recovery%20of%20protein%20from%20dairy%20milk%20waste%20product%20using%20alcohol-salt%20liquid%20biphasic%20flotation%20.pdf |
| _version_ | 1825951506949472256 |
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| author | Pei, En Tham Yan, Jer Ng Sankaran, Revathy Kuan, Shiong Khoo Kit, Wayne Chew Yee, Jiun Yap Malahubban, Masnindah Zakry, Fitri Abdul Aziz Pau, Loke Show |
| author_facet | Pei, En Tham Yan, Jer Ng Sankaran, Revathy Kuan, Shiong Khoo Kit, Wayne Chew Yee, Jiun Yap Malahubban, Masnindah Zakry, Fitri Abdul Aziz Pau, Loke Show |
| author_sort | Pei, En Tham |
| collection | UPM |
| description | Expired dairy products are often disposed of due to the potential health hazard they pose to living organisms. Lack of methods to recover valuable components from them are also a reason for manufactures to dispose of the expired dairy products. Milk encompasses several different components with their own functional properties that can be applied in production of food and non-food technical products. This study aims to investigate the novel approach of using liquid biphasic flotation (LBF) method for protein extraction from expired milk products and obtaining the optimal operating conditions for protein extraction. The optimized conditions were found at 80% concentration ethanol as top phase, 150 g/L dipotassium hydrogen phosphate along with 10% (w/v) milk as bottom phase, and a flotation time of 7.5 min. The protein recovery yield and separation efficiency after optimization were 94.97% and 86.289%, respectively. The experiment has been scaled up by 40 times to ensure it can be commercialized, and the protein recovery yield and separation efficiency were found to be 78.92% and 85.62%, respectively. This novel approach gives a chance for expired milk products to be changed from waste to raw materials which is beneficial for the environment and the economy. |
| first_indexed | 2024-03-06T10:31:01Z |
| format | Article |
| id | upm.eprints-81946 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:31:01Z |
| publishDate | 2019 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-819462021-09-29T01:34:03Z http://psasir.upm.edu.my/id/eprint/81946/ Recovery of protein from dairy milk waste product using alcohol-salt liquid biphasic flotation Pei, En Tham Yan, Jer Ng Sankaran, Revathy Kuan, Shiong Khoo Kit, Wayne Chew Yee, Jiun Yap Malahubban, Masnindah Zakry, Fitri Abdul Aziz Pau, Loke Show Expired dairy products are often disposed of due to the potential health hazard they pose to living organisms. Lack of methods to recover valuable components from them are also a reason for manufactures to dispose of the expired dairy products. Milk encompasses several different components with their own functional properties that can be applied in production of food and non-food technical products. This study aims to investigate the novel approach of using liquid biphasic flotation (LBF) method for protein extraction from expired milk products and obtaining the optimal operating conditions for protein extraction. The optimized conditions were found at 80% concentration ethanol as top phase, 150 g/L dipotassium hydrogen phosphate along with 10% (w/v) milk as bottom phase, and a flotation time of 7.5 min. The protein recovery yield and separation efficiency after optimization were 94.97% and 86.289%, respectively. The experiment has been scaled up by 40 times to ensure it can be commercialized, and the protein recovery yield and separation efficiency were found to be 78.92% and 85.62%, respectively. This novel approach gives a chance for expired milk products to be changed from waste to raw materials which is beneficial for the environment and the economy. Multidisciplinary Digital Publishing Institute 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81946/1/Recovery%20of%20protein%20from%20dairy%20milk%20waste%20product%20using%20alcohol-salt%20liquid%20biphasic%20flotation%20.pdf Pei, En Tham and Yan, Jer Ng and Sankaran, Revathy and Kuan, Shiong Khoo and Kit, Wayne Chew and Yee, Jiun Yap and Malahubban, Masnindah and Zakry, Fitri Abdul Aziz and Pau, Loke Show (2019) Recovery of protein from dairy milk waste product using alcohol-salt liquid biphasic flotation. Processes, 7 (12). art. no. 875. pp. 1-18. ISSN 2227-9717 https://www.mdpi.com/2227-9717/7/12/875 10.3390/pr7120875 |
| spellingShingle | Pei, En Tham Yan, Jer Ng Sankaran, Revathy Kuan, Shiong Khoo Kit, Wayne Chew Yee, Jiun Yap Malahubban, Masnindah Zakry, Fitri Abdul Aziz Pau, Loke Show Recovery of protein from dairy milk waste product using alcohol-salt liquid biphasic flotation |
| title | Recovery of protein from dairy milk waste product using alcohol-salt liquid biphasic flotation |
| title_full | Recovery of protein from dairy milk waste product using alcohol-salt liquid biphasic flotation |
| title_fullStr | Recovery of protein from dairy milk waste product using alcohol-salt liquid biphasic flotation |
| title_full_unstemmed | Recovery of protein from dairy milk waste product using alcohol-salt liquid biphasic flotation |
| title_short | Recovery of protein from dairy milk waste product using alcohol-salt liquid biphasic flotation |
| title_sort | recovery of protein from dairy milk waste product using alcohol salt liquid biphasic flotation |
| url | http://psasir.upm.edu.my/id/eprint/81946/1/Recovery%20of%20protein%20from%20dairy%20milk%20waste%20product%20using%20alcohol-salt%20liquid%20biphasic%20flotation%20.pdf |
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