Marker‐assisted introgression of broad‐spectrum blast resistance genes into the cultivated MR219 rice variety
BACKGROUND: The rice cultivar MR219 is famous for its better yield and long and fine grain quality; however, it is susceptible to blast disease. The main objective of this study was to introgress blast resistance genes into MR219 through marker‐assisted selection (MAS). The rice cultivar MR219 was u...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Society of Chemical Industry
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/8198/1/Marker%E2%80%90assisted%20introgression%20of%20broad%E2%80%90spectrum%20blast%20resistance%20genes%20into%20the%20cultivated%20MR219%20rice%20variety.pdf |
| _version_ | 1825944351484674048 |
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| author | Miah, Gous Yusop, Mohd Rafii Ismail, Mohd Razi Puteh, Adam Harun, Abdul Rahim Latif, Mohammad Abdul |
| author_facet | Miah, Gous Yusop, Mohd Rafii Ismail, Mohd Razi Puteh, Adam Harun, Abdul Rahim Latif, Mohammad Abdul |
| author_sort | Miah, Gous |
| collection | UPM |
| description | BACKGROUND: The rice cultivar MR219 is famous for its better yield and long and fine grain quality; however, it is susceptible to blast disease. The main objective of this study was to introgress blast resistance genes into MR219 through marker‐assisted selection (MAS). The rice cultivar MR219 was used as the recurrent parent, and Pongsu Seribu 1 was used as the donor.
RESULTS: Marker‐assisted foreground selection was performed using RM6836 and RM8225 to identify plants possessing blast resistance genes. Seventy microsatellite markers were used to estimate recurrent parent genome (RPG) recovery. Our analysis led to the development of 13 improved blast resistant lines with Piz, Pi2 and Pi9 broad‐spectrum blast resistance genes and an MR219 genetic background. The RPG recovery of the selected improved lines was up to 97.70% with an average value of 95.98%. Selected improved lines showed a resistance response against the most virulent blast pathogen pathotype, P7.2. The selected improved lines did not express any negative effect on agronomic traits in comparison with MR219.
CONCLUSION: The research findings of this study will be a conducive approach for the application of different molecular techniques that may result in accelerating the development of new disease‐resistant rice varieties, which in turn will match rising demand and food security worldwide. |
| first_indexed | 2024-03-06T07:14:31Z |
| format | Article |
| id | upm.eprints-8198 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:14:31Z |
| publishDate | 2017 |
| publisher | Society of Chemical Industry |
| record_format | dspace |
| spelling | upm.eprints-81982018-06-11T08:33:13Z http://psasir.upm.edu.my/id/eprint/8198/ Marker‐assisted introgression of broad‐spectrum blast resistance genes into the cultivated MR219 rice variety Miah, Gous Yusop, Mohd Rafii Ismail, Mohd Razi Puteh, Adam Harun, Abdul Rahim Latif, Mohammad Abdul BACKGROUND: The rice cultivar MR219 is famous for its better yield and long and fine grain quality; however, it is susceptible to blast disease. The main objective of this study was to introgress blast resistance genes into MR219 through marker‐assisted selection (MAS). The rice cultivar MR219 was used as the recurrent parent, and Pongsu Seribu 1 was used as the donor. RESULTS: Marker‐assisted foreground selection was performed using RM6836 and RM8225 to identify plants possessing blast resistance genes. Seventy microsatellite markers were used to estimate recurrent parent genome (RPG) recovery. Our analysis led to the development of 13 improved blast resistant lines with Piz, Pi2 and Pi9 broad‐spectrum blast resistance genes and an MR219 genetic background. The RPG recovery of the selected improved lines was up to 97.70% with an average value of 95.98%. Selected improved lines showed a resistance response against the most virulent blast pathogen pathotype, P7.2. The selected improved lines did not express any negative effect on agronomic traits in comparison with MR219. CONCLUSION: The research findings of this study will be a conducive approach for the application of different molecular techniques that may result in accelerating the development of new disease‐resistant rice varieties, which in turn will match rising demand and food security worldwide. Society of Chemical Industry 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/8198/1/Marker%E2%80%90assisted%20introgression%20of%20broad%E2%80%90spectrum%20blast%20resistance%20genes%20into%20the%20cultivated%20MR219%20rice%20variety.pdf Miah, Gous and Yusop, Mohd Rafii and Ismail, Mohd Razi and Puteh, Adam and Harun, Abdul Rahim and Latif, Mohammad Abdul (2017) Marker‐assisted introgression of broad‐spectrum blast resistance genes into the cultivated MR219 rice variety. Journal of the Science of Food and Agriculture, 97 (9). pp. 2810-2818. ISSN 0022-5142; ESSN: 1097-0010 https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.8109 10.1002/jsfa.8109 |
| spellingShingle | Miah, Gous Yusop, Mohd Rafii Ismail, Mohd Razi Puteh, Adam Harun, Abdul Rahim Latif, Mohammad Abdul Marker‐assisted introgression of broad‐spectrum blast resistance genes into the cultivated MR219 rice variety |
| title | Marker‐assisted introgression of broad‐spectrum blast resistance genes into the cultivated MR219 rice variety |
| title_full | Marker‐assisted introgression of broad‐spectrum blast resistance genes into the cultivated MR219 rice variety |
| title_fullStr | Marker‐assisted introgression of broad‐spectrum blast resistance genes into the cultivated MR219 rice variety |
| title_full_unstemmed | Marker‐assisted introgression of broad‐spectrum blast resistance genes into the cultivated MR219 rice variety |
| title_short | Marker‐assisted introgression of broad‐spectrum blast resistance genes into the cultivated MR219 rice variety |
| title_sort | marker assisted introgression of broad spectrum blast resistance genes into the cultivated mr219 rice variety |
| url | http://psasir.upm.edu.my/id/eprint/8198/1/Marker%E2%80%90assisted%20introgression%20of%20broad%E2%80%90spectrum%20blast%20resistance%20genes%20into%20the%20cultivated%20MR219%20rice%20variety.pdf |
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