Screening of three phenol-degrading bacteria for bioremoval of copper
Aim: Potential microbial isolates for bioremoval of copper were collected from phenolic and heavy metal-contaminated soils and screened in copper-containing medium for determining the maximum tolerance level for copper.? Methodology: Bioremoval of copper was assessed using sodium diethyl dithiocarba...
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| Format: | Article |
| Language: | English |
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Triveni Enterprises
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/82071/1/Screening%20of%20three%20phenol-degrading%20bacteria%20.pdf |
| _version_ | 1796980998561333248 |
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| author | Abdul Wahab Shaarani, Shakirah Abd.Shukor, Mohd Yunus Basirun, Ain Aqilah Ahmad, Siti Aqlima |
| author_facet | Abdul Wahab Shaarani, Shakirah Abd.Shukor, Mohd Yunus Basirun, Ain Aqilah Ahmad, Siti Aqlima |
| author_sort | Abdul Wahab Shaarani, Shakirah |
| collection | UPM |
| description | Aim: Potential microbial isolates for bioremoval of copper were collected from phenolic and heavy metal-contaminated soils and screened in copper-containing medium for determining the maximum tolerance level for copper.? Methodology: Bioremoval of copper was assessed using sodium diethyl dithiocarbamate assay. Physical and cultural conditions influencing copper bioremoval such as initial concentration, biomass dosage (inoculum volume), temperature and pH were optimised via one-factor-at-a-time. Results: The highest maximum tolerance level was displayed by Serratia sp. AQ5-03 at 600 mg l-1, while for Alcaligenes sp. AQ5-02 and Pseudomonas sp. AQ5-04 it was 500 mg l-1. The highest bioremoval for Alcaligenes sp. AQ5-02, Serratia sp. AQ5-03 and Pseudomonas sp. AQ5-04 was achieved at 20, 50 and 75 mg l-1, respectively. The optimum biomass dosage (inoculum volume) for both Serratia sp. AQ5-03 and Pseudomonas sp. AQ5-04 were 15%, whereas it was 10% for Alcaligenes sp. AQ5-02. The results also revealed that maximum bioremoval was achieved at pH 6 at an optimum temperature of 20?C for both Alcaligenes sp. AQ5-02 and Pseudomonas sp. AQ5-04, while Serratia sp. AQ5-03 showed optimum removal at pH 7 and at 30?C temperature. The maximum bioremoval efficiency for Alcaligenes sp. AQ5-02, Serratia sp. AQ5-03 and Pseudomonas sp were found to be 62, 57 and 70%, respectively. Interpretation: The results indicate that Alcaligenes sp. AQ5-02, Serratia sp. AQ5-03, Pseudomonas sp. AQ5-04 can be utilised as bioremoval agent since it possessed the highest bioremoval efficiency for copper remediation. |
| first_indexed | 2024-03-06T10:31:17Z |
| format | Article |
| id | upm.eprints-82071 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:31:17Z |
| publishDate | 2019 |
| publisher | Triveni Enterprises |
| record_format | dspace |
| spelling | upm.eprints-820712021-09-08T21:13:32Z http://psasir.upm.edu.my/id/eprint/82071/ Screening of three phenol-degrading bacteria for bioremoval of copper Abdul Wahab Shaarani, Shakirah Abd.Shukor, Mohd Yunus Basirun, Ain Aqilah Ahmad, Siti Aqlima Aim: Potential microbial isolates for bioremoval of copper were collected from phenolic and heavy metal-contaminated soils and screened in copper-containing medium for determining the maximum tolerance level for copper.? Methodology: Bioremoval of copper was assessed using sodium diethyl dithiocarbamate assay. Physical and cultural conditions influencing copper bioremoval such as initial concentration, biomass dosage (inoculum volume), temperature and pH were optimised via one-factor-at-a-time. Results: The highest maximum tolerance level was displayed by Serratia sp. AQ5-03 at 600 mg l-1, while for Alcaligenes sp. AQ5-02 and Pseudomonas sp. AQ5-04 it was 500 mg l-1. The highest bioremoval for Alcaligenes sp. AQ5-02, Serratia sp. AQ5-03 and Pseudomonas sp. AQ5-04 was achieved at 20, 50 and 75 mg l-1, respectively. The optimum biomass dosage (inoculum volume) for both Serratia sp. AQ5-03 and Pseudomonas sp. AQ5-04 were 15%, whereas it was 10% for Alcaligenes sp. AQ5-02. The results also revealed that maximum bioremoval was achieved at pH 6 at an optimum temperature of 20?C for both Alcaligenes sp. AQ5-02 and Pseudomonas sp. AQ5-04, while Serratia sp. AQ5-03 showed optimum removal at pH 7 and at 30?C temperature. The maximum bioremoval efficiency for Alcaligenes sp. AQ5-02, Serratia sp. AQ5-03 and Pseudomonas sp were found to be 62, 57 and 70%, respectively. Interpretation: The results indicate that Alcaligenes sp. AQ5-02, Serratia sp. AQ5-03, Pseudomonas sp. AQ5-04 can be utilised as bioremoval agent since it possessed the highest bioremoval efficiency for copper remediation. Triveni Enterprises 2019-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82071/1/Screening%20of%20three%20phenol-degrading%20bacteria%20.pdf Abdul Wahab Shaarani, Shakirah and Abd.Shukor, Mohd Yunus and Basirun, Ain Aqilah and Ahmad, Siti Aqlima (2019) Screening of three phenol-degrading bacteria for bioremoval of copper. Journal of Environmental Biology, 40 (5). pp. 1045-1051. ISSN 0254-8704; ESSN: 2394-0378 https://www.cabdirect.org/cabdirect/abstract/20219939301 10.22438/jeb/40/5/MRN-803 |
| spellingShingle | Abdul Wahab Shaarani, Shakirah Abd.Shukor, Mohd Yunus Basirun, Ain Aqilah Ahmad, Siti Aqlima Screening of three phenol-degrading bacteria for bioremoval of copper |
| title | Screening of three phenol-degrading bacteria for bioremoval of copper |
| title_full | Screening of three phenol-degrading bacteria for bioremoval of copper |
| title_fullStr | Screening of three phenol-degrading bacteria for bioremoval of copper |
| title_full_unstemmed | Screening of three phenol-degrading bacteria for bioremoval of copper |
| title_short | Screening of three phenol-degrading bacteria for bioremoval of copper |
| title_sort | screening of three phenol degrading bacteria for bioremoval of copper |
| url | http://psasir.upm.edu.my/id/eprint/82071/1/Screening%20of%20three%20phenol-degrading%20bacteria%20.pdf |
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