Sensitive surface plasmon resonance performance of cadmium sulfide quantum dots-amine functionalized graphene oxide based thin film towards dengue virus E-protein
An optical sensor for the dengue virus (DENV) E-protein based on cadmium sulfide quantum dots composited with amine functionalized graphene oxide (CdS-NH2GO) thin film was successfully developed. A specific monoclonal antibodies (IgM) were covalently attached to CdS-NH2GO via EDC/NHS coupling to sen...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/82088/1/Sensitive%20surface%20plasmon%20resonance%20.pdf |
| _version_ | 1796981001467985920 |
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| author | Sheh Omar, Nur Alia Yap, Wing Fen Abdullah, Jaafar Mat Zaid, Mohd Hazani Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Mahdi, Mohd Adzir |
| author_facet | Sheh Omar, Nur Alia Yap, Wing Fen Abdullah, Jaafar Mat Zaid, Mohd Hazani Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Mahdi, Mohd Adzir |
| author_sort | Sheh Omar, Nur Alia |
| collection | UPM |
| description | An optical sensor for the dengue virus (DENV) E-protein based on cadmium sulfide quantum dots composited with amine functionalized graphene oxide (CdS-NH2GO) thin film was successfully developed. A specific monoclonal antibodies (IgM) were covalently attached to CdS-NH2GO via EDC/NHS coupling to sense targeted E-proteins. The SPR sensor exhibited an excellent detection limit (0.001 nM/1 pM) with sensitivity of 5.49° nM−1 for the detection of DENV E-protein. The binding affinity, as well as the performance of the Au/CdS-NH2GO/EDC-NHS/IgM film, was successfully obtained at 486.54 nM−1 in detecting DENV E-proteins. These results indicated that the Au/CdS-NH2GO/EDC-NHS/IgM film shows high potential sensitive and stronger binding towards DENV E-protein. |
| first_indexed | 2024-03-06T10:31:19Z |
| format | Article |
| id | upm.eprints-82088 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:31:19Z |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-820882021-09-08T21:41:58Z http://psasir.upm.edu.my/id/eprint/82088/ Sensitive surface plasmon resonance performance of cadmium sulfide quantum dots-amine functionalized graphene oxide based thin film towards dengue virus E-protein Sheh Omar, Nur Alia Yap, Wing Fen Abdullah, Jaafar Mat Zaid, Mohd Hazani Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Mahdi, Mohd Adzir An optical sensor for the dengue virus (DENV) E-protein based on cadmium sulfide quantum dots composited with amine functionalized graphene oxide (CdS-NH2GO) thin film was successfully developed. A specific monoclonal antibodies (IgM) were covalently attached to CdS-NH2GO via EDC/NHS coupling to sense targeted E-proteins. The SPR sensor exhibited an excellent detection limit (0.001 nM/1 pM) with sensitivity of 5.49° nM−1 for the detection of DENV E-protein. The binding affinity, as well as the performance of the Au/CdS-NH2GO/EDC-NHS/IgM film, was successfully obtained at 486.54 nM−1 in detecting DENV E-proteins. These results indicated that the Au/CdS-NH2GO/EDC-NHS/IgM film shows high potential sensitive and stronger binding towards DENV E-protein. Elsevier 2019-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82088/1/Sensitive%20surface%20plasmon%20resonance%20.pdf Sheh Omar, Nur Alia and Yap, Wing Fen and Abdullah, Jaafar and Mat Zaid, Mohd Hazani and Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim and Mahdi, Mohd Adzir (2019) Sensitive surface plasmon resonance performance of cadmium sulfide quantum dots-amine functionalized graphene oxide based thin film towards dengue virus E-protein. Optics & Laser Technology, 114. pp. 204-208. ISSN 0030-3992; ESSN: 1879-2545 https://www.sciencedirect.com/science/article/abs/pii/S0030399218306819 10.1016/j.optlastec.2019.01.038 |
| spellingShingle | Sheh Omar, Nur Alia Yap, Wing Fen Abdullah, Jaafar Mat Zaid, Mohd Hazani Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Mahdi, Mohd Adzir Sensitive surface plasmon resonance performance of cadmium sulfide quantum dots-amine functionalized graphene oxide based thin film towards dengue virus E-protein |
| title | Sensitive surface plasmon resonance performance of cadmium sulfide quantum dots-amine functionalized graphene oxide based thin film towards dengue virus E-protein |
| title_full | Sensitive surface plasmon resonance performance of cadmium sulfide quantum dots-amine functionalized graphene oxide based thin film towards dengue virus E-protein |
| title_fullStr | Sensitive surface plasmon resonance performance of cadmium sulfide quantum dots-amine functionalized graphene oxide based thin film towards dengue virus E-protein |
| title_full_unstemmed | Sensitive surface plasmon resonance performance of cadmium sulfide quantum dots-amine functionalized graphene oxide based thin film towards dengue virus E-protein |
| title_short | Sensitive surface plasmon resonance performance of cadmium sulfide quantum dots-amine functionalized graphene oxide based thin film towards dengue virus E-protein |
| title_sort | sensitive surface plasmon resonance performance of cadmium sulfide quantum dots amine functionalized graphene oxide based thin film towards dengue virus e protein |
| url | http://psasir.upm.edu.my/id/eprint/82088/1/Sensitive%20surface%20plasmon%20resonance%20.pdf |
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