The investigation of media components for optimal metabolite production of Aspergillus terreus ATCC 20542
Purpose: This study aimed to assess the effect of nitrogen, salt and pre-culture conditions on the production of lovastatin in A. terreus ATCC 20542. Methods: Different combinations of nitrogen sources, salts and pre-culture combinations were applied in the fermentation media and lovastatin yield wa...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/82535/1/The%20investigation%20of%20media%20components%20for%20optimal%20metabolite%20production%20of%20Aspergillus%20terreus%20ATCC%2020542.pdf |
| _version_ | 1825951570604326912 |
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| author | Abd Rahim, Muhamad Hafiz Lim, Elicia Jitming Hasan, Hanan Abbas, Ali |
| author_facet | Abd Rahim, Muhamad Hafiz Lim, Elicia Jitming Hasan, Hanan Abbas, Ali |
| author_sort | Abd Rahim, Muhamad Hafiz |
| collection | UPM |
| description | Purpose: This study aimed to assess the effect of nitrogen, salt and pre-culture conditions on the production of lovastatin in A. terreus ATCC 20542. Methods: Different combinations of nitrogen sources, salts and pre-culture combinations were applied in the fermentation media and lovastatin yield was analysed chromatographically. Result: The exclusion of MnSO₄ ·5H₂O, CuSO₄·5H₂O and FeCl₃·6H₂O were shown to significantly improve lovastatin production (282%), while KH2PO₄, MgSO₄·7H₂O, and NaCl and ZnSO4·7H₂O were indispensable for good lovastatin production. Simple nitrogen source (ammonia) was unfavourable for morphology, growth and lovastatin production. In contrast, yeast extract (complex nitrogen source) produced the highest lovastatin yield (25.52 mg/L), while powdered soybean favoured the production of co-metabolites ((+)-geodin and sulochrin). Intermediate lactose: yeast extract (5:4) ratio produced the optimal lovastatin yield (12.33 mg/L) during pre-culture, while high (5:2) or low (5:6) lactose to yeast extract ratio produced significantly lower lovastatin yield (7.98 mg/L and 9.12 mg/L, respectively). High spore concentration, up to 107 spores/L was shown to be beneficial for lovastatin, but not for co-metabolite production, while higher spore age was shown to be beneficial for all of its metabolites. Conclusion: The findings from these investigations could be used for future cultivation of A. terreus in the production of desired metabolites. |
| first_indexed | 2024-03-06T10:31:59Z |
| format | Article |
| id | upm.eprints-82535 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:31:59Z |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-825352021-08-08T00:13:22Z http://psasir.upm.edu.my/id/eprint/82535/ The investigation of media components for optimal metabolite production of Aspergillus terreus ATCC 20542 Abd Rahim, Muhamad Hafiz Lim, Elicia Jitming Hasan, Hanan Abbas, Ali Purpose: This study aimed to assess the effect of nitrogen, salt and pre-culture conditions on the production of lovastatin in A. terreus ATCC 20542. Methods: Different combinations of nitrogen sources, salts and pre-culture combinations were applied in the fermentation media and lovastatin yield was analysed chromatographically. Result: The exclusion of MnSO₄ ·5H₂O, CuSO₄·5H₂O and FeCl₃·6H₂O were shown to significantly improve lovastatin production (282%), while KH2PO₄, MgSO₄·7H₂O, and NaCl and ZnSO4·7H₂O were indispensable for good lovastatin production. Simple nitrogen source (ammonia) was unfavourable for morphology, growth and lovastatin production. In contrast, yeast extract (complex nitrogen source) produced the highest lovastatin yield (25.52 mg/L), while powdered soybean favoured the production of co-metabolites ((+)-geodin and sulochrin). Intermediate lactose: yeast extract (5:4) ratio produced the optimal lovastatin yield (12.33 mg/L) during pre-culture, while high (5:2) or low (5:6) lactose to yeast extract ratio produced significantly lower lovastatin yield (7.98 mg/L and 9.12 mg/L, respectively). High spore concentration, up to 107 spores/L was shown to be beneficial for lovastatin, but not for co-metabolite production, while higher spore age was shown to be beneficial for all of its metabolites. Conclusion: The findings from these investigations could be used for future cultivation of A. terreus in the production of desired metabolites. Elsevier 2019-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82535/1/The%20investigation%20of%20media%20components%20for%20optimal%20metabolite%20production%20of%20Aspergillus%20terreus%20ATCC%2020542.pdf Abd Rahim, Muhamad Hafiz and Lim, Elicia Jitming and Hasan, Hanan and Abbas, Ali (2019) The investigation of media components for optimal metabolite production of Aspergillus terreus ATCC 20542. Journal of Microbiological Methods, 164. pp. 1-6. ISSN 0167-7012; ESSN: 1872-8359 https://www.sciencedirect.com/science/article/abs/pii/S016770121930510X 10.1016/j.mimet.2019.105672 |
| spellingShingle | Abd Rahim, Muhamad Hafiz Lim, Elicia Jitming Hasan, Hanan Abbas, Ali The investigation of media components for optimal metabolite production of Aspergillus terreus ATCC 20542 |
| title | The investigation of media components for optimal metabolite production of Aspergillus terreus ATCC 20542 |
| title_full | The investigation of media components for optimal metabolite production of Aspergillus terreus ATCC 20542 |
| title_fullStr | The investigation of media components for optimal metabolite production of Aspergillus terreus ATCC 20542 |
| title_full_unstemmed | The investigation of media components for optimal metabolite production of Aspergillus terreus ATCC 20542 |
| title_short | The investigation of media components for optimal metabolite production of Aspergillus terreus ATCC 20542 |
| title_sort | investigation of media components for optimal metabolite production of aspergillus terreus atcc 20542 |
| url | http://psasir.upm.edu.my/id/eprint/82535/1/The%20investigation%20of%20media%20components%20for%20optimal%20metabolite%20production%20of%20Aspergillus%20terreus%20ATCC%2020542.pdf |
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