The role of oxidative stress in Brachiaria decumbens toxicity in sheep
In an attempt to elucidate potential time-dependent oxidative stress mechanisms associated with Brachiaria decumbens toxicity in sheep, selected blood malondialdehyde (MD), as peroxidation tissue function biomarker and tissue morphology, were assessed. Six young adult Wiltshire cross bred ram were a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/8286/1/24%20Pg%20151-157.pdf |
| _version_ | 1796967496394211328 |
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| author | Assumaidaee, Ajwad Awad Mohammed Saad, Mohd Zamri Sabri, Jasni Mohamed Mustapha, Noordin |
| author_facet | Assumaidaee, Ajwad Awad Mohammed Saad, Mohd Zamri Sabri, Jasni Mohamed Mustapha, Noordin |
| author_sort | Assumaidaee, Ajwad Awad Mohammed |
| collection | UPM |
| description | In an attempt to elucidate potential time-dependent oxidative stress mechanisms associated with Brachiaria decumbens toxicity in sheep, selected blood malondialdehyde (MD), as peroxidation tissue function biomarker and tissue morphology, were assessed. Six young adult Wiltshire cross bred ram were acclimatized for 3 weeks, where ivermectin injection and liver and kidney function tests were done. Blood samples were collected during the pre-treatment, namely on 3rd, 5th, 7th, 9th, and 11th days of continuous feeding of Brachiaria. Meanwhile, clinical signs were monitored and sheep were euthanised on the 7th, 9th, and 11th day, where a post-mortem was performed and relevant tissues were sampled. Results revealed that plasma thiobarbituric acid reactive substances (TBARS), serum (bilirubin total and conjugated, AST, GGT, urea, and creatinine) were significantly (p<0.05) increased, whereas total antioxidant potential was decreased (p<0.05) in a time dependant manner. Continuous B. decumbens feeding induced a time dependent appearance of jaundice, photosensitization, and subcutaneous oedema. Unique intracytoplasmic accumulation of Schmorl's positive greenish lipofuscin granules were observed primarily within the centrilobular hepatocytes and Kupffer cells. This may serve as a histochemical oxidative biomarker in the liver, kidney, brain, and skin. Taken together, this study shows that oxidative stress plays a major role in B. decumbens toxicity. |
| first_indexed | 2024-03-06T07:14:46Z |
| format | Article |
| id | upm.eprints-8286 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:14:46Z |
| publishDate | 2010 |
| publisher | Universiti Putra Malaysia Press |
| record_format | dspace |
| spelling | upm.eprints-82862016-11-21T08:02:01Z http://psasir.upm.edu.my/id/eprint/8286/ The role of oxidative stress in Brachiaria decumbens toxicity in sheep Assumaidaee, Ajwad Awad Mohammed Saad, Mohd Zamri Sabri, Jasni Mohamed Mustapha, Noordin In an attempt to elucidate potential time-dependent oxidative stress mechanisms associated with Brachiaria decumbens toxicity in sheep, selected blood malondialdehyde (MD), as peroxidation tissue function biomarker and tissue morphology, were assessed. Six young adult Wiltshire cross bred ram were acclimatized for 3 weeks, where ivermectin injection and liver and kidney function tests were done. Blood samples were collected during the pre-treatment, namely on 3rd, 5th, 7th, 9th, and 11th days of continuous feeding of Brachiaria. Meanwhile, clinical signs were monitored and sheep were euthanised on the 7th, 9th, and 11th day, where a post-mortem was performed and relevant tissues were sampled. Results revealed that plasma thiobarbituric acid reactive substances (TBARS), serum (bilirubin total and conjugated, AST, GGT, urea, and creatinine) were significantly (p<0.05) increased, whereas total antioxidant potential was decreased (p<0.05) in a time dependant manner. Continuous B. decumbens feeding induced a time dependent appearance of jaundice, photosensitization, and subcutaneous oedema. Unique intracytoplasmic accumulation of Schmorl's positive greenish lipofuscin granules were observed primarily within the centrilobular hepatocytes and Kupffer cells. This may serve as a histochemical oxidative biomarker in the liver, kidney, brain, and skin. Taken together, this study shows that oxidative stress plays a major role in B. decumbens toxicity. Universiti Putra Malaysia Press 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/8286/1/24%20Pg%20151-157.pdf Assumaidaee, Ajwad Awad Mohammed and Saad, Mohd Zamri and Sabri, Jasni and Mohamed Mustapha, Noordin (2010) The role of oxidative stress in Brachiaria decumbens toxicity in sheep. Pertanika Journal of Tropical Agricultural Science, 33 (1). pp. 151-157. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2033%20(1)%20Feb.%202010/24%20Pg%20151-157.pdf |
| spellingShingle | Assumaidaee, Ajwad Awad Mohammed Saad, Mohd Zamri Sabri, Jasni Mohamed Mustapha, Noordin The role of oxidative stress in Brachiaria decumbens toxicity in sheep |
| title | The role of oxidative stress in Brachiaria decumbens toxicity in sheep |
| title_full | The role of oxidative stress in Brachiaria decumbens toxicity in sheep |
| title_fullStr | The role of oxidative stress in Brachiaria decumbens toxicity in sheep |
| title_full_unstemmed | The role of oxidative stress in Brachiaria decumbens toxicity in sheep |
| title_short | The role of oxidative stress in Brachiaria decumbens toxicity in sheep |
| title_sort | role of oxidative stress in brachiaria decumbens toxicity in sheep |
| url | http://psasir.upm.edu.my/id/eprint/8286/1/24%20Pg%20151-157.pdf |
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