L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase
Enzymatic resolution of DL-amino acids using aminoacylase immobilised on DEAE-sephadex has been employed in the industry for the production of the optically active, L-amino acid There are,however, soeliitations to this technique. The aims of this study were to establish an efficient immobilisatio...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1993
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| Online Access: | http://psasir.upm.edu.my/id/eprint/8358/1/FSMB_1993_2_A.pdf |
| _version_ | 1825944381196075008 |
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| author | Siaw, Yew Seong |
| author_facet | Siaw, Yew Seong |
| author_sort | Siaw, Yew Seong |
| collection | UPM |
| description | Enzymatic resolution of DL-amino acids using aminoacylase
immobilised on DEAE-sephadex has been employed in the industry
for the production of the optically active, L-amino acid
There are,however, soeliitations to this technique. The aims of this study were to establish an efficient immobilisation method for aminoacylase and to report the
preparation, properties, and applicability of the resulting
immobilised aminoacylase. Aminoacylase was immobilised in
calcium alginate beads(ACAG). The ACAG was further stabilised
by coating with polyethyleneimine (PEI), a mixture of
polyethyleneimine and glutaraldehyde(PEI-GLU), poly-L-lysine
(PLL), or by cross - linking with carbodiimide. Each type of the
immobilised aminoacylase was then used to perform the optical
resolution of N-acetyl-DL-phenylalanine. |
| first_indexed | 2024-03-06T07:14:59Z |
| format | Thesis |
| id | upm.eprints-8358 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:14:59Z |
| publishDate | 1993 |
| record_format | dspace |
| spelling | upm.eprints-83582012-11-02T02:12:32Z http://psasir.upm.edu.my/id/eprint/8358/ L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase Siaw, Yew Seong Enzymatic resolution of DL-amino acids using aminoacylase immobilised on DEAE-sephadex has been employed in the industry for the production of the optically active, L-amino acid There are,however, soeliitations to this technique. The aims of this study were to establish an efficient immobilisation method for aminoacylase and to report the preparation, properties, and applicability of the resulting immobilised aminoacylase. Aminoacylase was immobilised in calcium alginate beads(ACAG). The ACAG was further stabilised by coating with polyethyleneimine (PEI), a mixture of polyethyleneimine and glutaraldehyde(PEI-GLU), poly-L-lysine (PLL), or by cross - linking with carbodiimide. Each type of the immobilised aminoacylase was then used to perform the optical resolution of N-acetyl-DL-phenylalanine. 1993 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/8358/1/FSMB_1993_2_A.pdf Siaw, Yew Seong (1993) L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase. Masters thesis, Universiti Pertanian Malaysia. Immobilized enzymes Calcium - Stability English |
| spellingShingle | Immobilized enzymes Calcium - Stability Siaw, Yew Seong L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase |
| title | L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase |
| title_full | L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase |
| title_fullStr | L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase |
| title_full_unstemmed | L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase |
| title_short | L-Phenylalanine Production by Stabilised Calcium Alginate Immobilised Aminoacylase |
| title_sort | l phenylalanine production by stabilised calcium alginate immobilised aminoacylase |
| topic | Immobilized enzymes Calcium - Stability |
| url | http://psasir.upm.edu.my/id/eprint/8358/1/FSMB_1993_2_A.pdf |
| work_keys_str_mv | AT siawyewseong lphenylalanineproductionbystabilisedcalciumalginateimmobilisedaminoacylase |