Effects of Lactobacillus probiotic on growth performance and coccidiosis in broiler chickens challenged with Eimeria tenella
Avian coccidiosisis is an intestinal disease caused by several species of Eimeria (family Eimeriidae) of the phylum Apicomplexa. Probiotics had been evaluated as an alternative to anticoccidial drugs, and although the resistance of birds to coccidiosis was enhanced, but the protective effe...
| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/84817/1/IB%202019%2025%20-%20ir.pdf |
| _version_ | 1796981496276320256 |
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| author | Shamsudin, Wan Nur Fadhilah |
| author_facet | Shamsudin, Wan Nur Fadhilah |
| author_sort | Shamsudin, Wan Nur Fadhilah |
| collection | UPM |
| description | Avian coccidiosisis is an intestinal disease caused by several species of Eimeria (family
Eimeriidae) of the phylum Apicomplexa. Probiotics had been evaluated as an alternative to
anticoccidial drugs, and although the resistance of birds to coccidiosis was enhanced, but the
protective effects were not always associated with an improved growth performance.
Therefore, it was necessary to find probiotic species with anti-coccidiosis activity and at the
same time improved broilers growth performance when challenged with E. tenella. Five out of 46
lactic acid bacteria (LAB) isolates from chicken intestine demonstrated good tolerance to acidic pH
3, 0.3% bile salts and of these, three isolates showed strong adhesion to Caco-2 cells. These
three isolates also showed antagonistic activity against pathogenic E. coli (078:K80), Salmonella
enterica serovar Typhimurium,
C. perfringens (Type A), Salmonella enterica serovar Enteritidis, E. faecium (FM3), E. faecalis
(E227). All three isolates had high inhibition zone (> 6mm) against E. coli 078:K80 and
Salmonella enterica serovar Typhimurium and were susceptible to antibiotics gentamicin,
kanamycin, streptomycin, tetracycline, erythromycin, clindamycin, chloramphenicol and
ampicillin. These isolates were identified by using 16S rRNA sequencing technique and identified
as two L. salivarius and one L. reuteri with accession number MH375403, MH375402 and MH375404,
respectively. The LAB isolates were evaluated for their efficacy as anticoccidiosis
alternatives in broilers challenged with E tenella. Four dietary treatments: untreated,
unchallenged broilers fed basal diet (BD); treated with anti-coccidial drug (EimeriaX 12%) and
challenged (ANTI); untreated and challenged (COCCID); and treated with probiotic (Lactobacilli
mixture at 5x10⁸ CFU/kg) and challenged (PRO); were used in a feeding trial of 42 days with 40
chicks (Cobb 500) per treatment divided into 5 replicates. All groups, except BD, were challenged
with 2x10⁴/mL of E. tenella oocysts on day 14 of broilers age. The results showed that broilers fed
PRO diet had comparable body weight and feed conversion ratio (FCR) compared to BD and ANTI groups.
Broilers of COCCID group had significantly lower (P<0.05) body weight and higher FCR. The lesion
score was only detected in both infected chickens of PRO and COCCID groups at day 21 and was not
detected at day 42. Oocyst count was significantly higher (P<0.05) in the COCCID group, compared to
PRO and ANTI groups. The population of Lactobacillus spp. and Bifidobacterium spp., as well as villus
and crypt depth were significantly higher (P<0.05) in the PRO group, while the populations of E.
coli and C. perfringens were lower. In conclusion, the isolated LAB probiotics could improve
the growth performance and alleviate the effects of coccidiosis infection when challenged
with E. tenella, comparable to broilers treated
with anticoccidial drug. |
| first_indexed | 2024-03-06T10:38:26Z |
| format | Thesis |
| id | upm.eprints-84817 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:38:26Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | upm.eprints-848172021-12-31T03:20:37Z http://psasir.upm.edu.my/id/eprint/84817/ Effects of Lactobacillus probiotic on growth performance and coccidiosis in broiler chickens challenged with Eimeria tenella Shamsudin, Wan Nur Fadhilah Avian coccidiosisis is an intestinal disease caused by several species of Eimeria (family Eimeriidae) of the phylum Apicomplexa. Probiotics had been evaluated as an alternative to anticoccidial drugs, and although the resistance of birds to coccidiosis was enhanced, but the protective effects were not always associated with an improved growth performance. Therefore, it was necessary to find probiotic species with anti-coccidiosis activity and at the same time improved broilers growth performance when challenged with E. tenella. Five out of 46 lactic acid bacteria (LAB) isolates from chicken intestine demonstrated good tolerance to acidic pH 3, 0.3% bile salts and of these, three isolates showed strong adhesion to Caco-2 cells. These three isolates also showed antagonistic activity against pathogenic E. coli (078:K80), Salmonella enterica serovar Typhimurium, C. perfringens (Type A), Salmonella enterica serovar Enteritidis, E. faecium (FM3), E. faecalis (E227). All three isolates had high inhibition zone (> 6mm) against E. coli 078:K80 and Salmonella enterica serovar Typhimurium and were susceptible to antibiotics gentamicin, kanamycin, streptomycin, tetracycline, erythromycin, clindamycin, chloramphenicol and ampicillin. These isolates were identified by using 16S rRNA sequencing technique and identified as two L. salivarius and one L. reuteri with accession number MH375403, MH375402 and MH375404, respectively. The LAB isolates were evaluated for their efficacy as anticoccidiosis alternatives in broilers challenged with E tenella. Four dietary treatments: untreated, unchallenged broilers fed basal diet (BD); treated with anti-coccidial drug (EimeriaX 12%) and challenged (ANTI); untreated and challenged (COCCID); and treated with probiotic (Lactobacilli mixture at 5x10⁸ CFU/kg) and challenged (PRO); were used in a feeding trial of 42 days with 40 chicks (Cobb 500) per treatment divided into 5 replicates. All groups, except BD, were challenged with 2x10⁴/mL of E. tenella oocysts on day 14 of broilers age. The results showed that broilers fed PRO diet had comparable body weight and feed conversion ratio (FCR) compared to BD and ANTI groups. Broilers of COCCID group had significantly lower (P<0.05) body weight and higher FCR. The lesion score was only detected in both infected chickens of PRO and COCCID groups at day 21 and was not detected at day 42. Oocyst count was significantly higher (P<0.05) in the COCCID group, compared to PRO and ANTI groups. The population of Lactobacillus spp. and Bifidobacterium spp., as well as villus and crypt depth were significantly higher (P<0.05) in the PRO group, while the populations of E. coli and C. perfringens were lower. In conclusion, the isolated LAB probiotics could improve the growth performance and alleviate the effects of coccidiosis infection when challenged with E. tenella, comparable to broilers treated with anticoccidial drug. 2019-02 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/84817/1/IB%202019%2025%20-%20ir.pdf Shamsudin, Wan Nur Fadhilah (2019) Effects of Lactobacillus probiotic on growth performance and coccidiosis in broiler chickens challenged with Eimeria tenella. Masters thesis, Universiti Putra Malaysia. Lactobacillus Lactobacillus - Therapentic use Eimeria tenella |
| spellingShingle | Lactobacillus Lactobacillus - Therapentic use Eimeria tenella Shamsudin, Wan Nur Fadhilah Effects of Lactobacillus probiotic on growth performance and coccidiosis in broiler chickens challenged with Eimeria tenella |
| title | Effects of Lactobacillus probiotic on growth performance and coccidiosis in broiler chickens challenged with Eimeria tenella |
| title_full | Effects of Lactobacillus probiotic on growth performance and coccidiosis in broiler chickens challenged with Eimeria tenella |
| title_fullStr | Effects of Lactobacillus probiotic on growth performance and coccidiosis in broiler chickens challenged with Eimeria tenella |
| title_full_unstemmed | Effects of Lactobacillus probiotic on growth performance and coccidiosis in broiler chickens challenged with Eimeria tenella |
| title_short | Effects of Lactobacillus probiotic on growth performance and coccidiosis in broiler chickens challenged with Eimeria tenella |
| title_sort | effects of lactobacillus probiotic on growth performance and coccidiosis in broiler chickens challenged with eimeria tenella |
| topic | Lactobacillus Lactobacillus - Therapentic use Eimeria tenella |
| url | http://psasir.upm.edu.my/id/eprint/84817/1/IB%202019%2025%20-%20ir.pdf |
| work_keys_str_mv | AT shamsudinwannurfadhilah effectsoflactobacillusprobioticongrowthperformanceandcoccidiosisinbroilerchickenschallengedwitheimeriatenella |