The assessment of cholinesterase from gills of Anabus testudineus as detection of metal ions
Cholinesterase from the gill of Anabus testudineus contains mostly AChE. It was partially purified by ammonium sulphate precipitation and DEAE cellulose using ion exchange chromatography with 4.890U of specific activity, 11.7 of purification fold and 3.0% yield. Optimum pH f...
| Main Author: | |
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/85074/1/FBSB%202015%2059%20-%20IR.pdf |
| _version_ | 1796981546412933120 |
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| author | Wong, Weini |
| author_facet | Wong, Weini |
| author_sort | Wong, Weini |
| collection | UPM |
| description | Cholinesterase from the gill of Anabus testudineus contains mostly AChE. It was
partially purified by ammonium sulphate precipitation and DEAE cellulose using ion exchange
chromatography with 4.890U of specific activity, 11.7 of purification fold and 3.0%
yield. Optimum pH for AChE in gill of A. testudineus is 8 using Tris-HCl buffer at
temperature 25°C. The optimum acetylthiocholine iodide concentration is 2.5 mM with Vmₐₓ
2.533 and Km 0.8802 in which the catalytic efficiency of 2.88. For metal ion inhibition, 10
metal ions were tested and AChE in gill of Anabus testudineus showed a critically low
enzyme activity towards mercury in which the activity is inhibited by 99.05%. However,
cobalt and silver had no inhibitory effect on AChE. From IC₅₀, only 0.0123 ppm of Hg
was required to reduce the enzyme activity by half. |
| first_indexed | 2024-03-06T10:39:11Z |
| format | Project Paper Report |
| id | upm.eprints-85074 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:39:11Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | upm.eprints-850742021-07-13T04:46:18Z http://psasir.upm.edu.my/id/eprint/85074/ The assessment of cholinesterase from gills of Anabus testudineus as detection of metal ions Wong, Weini Cholinesterase from the gill of Anabus testudineus contains mostly AChE. It was partially purified by ammonium sulphate precipitation and DEAE cellulose using ion exchange chromatography with 4.890U of specific activity, 11.7 of purification fold and 3.0% yield. Optimum pH for AChE in gill of A. testudineus is 8 using Tris-HCl buffer at temperature 25°C. The optimum acetylthiocholine iodide concentration is 2.5 mM with Vmₐₓ 2.533 and Km 0.8802 in which the catalytic efficiency of 2.88. For metal ion inhibition, 10 metal ions were tested and AChE in gill of Anabus testudineus showed a critically low enzyme activity towards mercury in which the activity is inhibited by 99.05%. However, cobalt and silver had no inhibitory effect on AChE. From IC₅₀, only 0.0123 ppm of Hg was required to reduce the enzyme activity by half. 2015-06 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/85074/1/FBSB%202015%2059%20-%20IR.pdf Wong, Weini (2015) The assessment of cholinesterase from gills of Anabus testudineus as detection of metal ions. [Project Paper Report] |
| spellingShingle | Wong, Weini The assessment of cholinesterase from gills of Anabus testudineus as detection of metal ions |
| title | The assessment of cholinesterase from gills of Anabus testudineus as detection of metal ions |
| title_full | The assessment of cholinesterase from gills of Anabus testudineus as detection of metal ions |
| title_fullStr | The assessment of cholinesterase from gills of Anabus testudineus as detection of metal ions |
| title_full_unstemmed | The assessment of cholinesterase from gills of Anabus testudineus as detection of metal ions |
| title_short | The assessment of cholinesterase from gills of Anabus testudineus as detection of metal ions |
| title_sort | assessment of cholinesterase from gills of anabus testudineus as detection of metal ions |
| url | http://psasir.upm.edu.my/id/eprint/85074/1/FBSB%202015%2059%20-%20IR.pdf |
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