Differential cell counts in 5 commercial ornamental fishes

The aim of this study was to determine the percentage of differential types of leukocytes in commercial ornamental fish (Green Tiger Barb - Puntigrus tetrazona, Rosy Tetra - Hyphessobrycon rosaceus, Rosy Barb - Pethia conchonius, Red Eye Tetra - Moenkhausia sanctaefilomenae and Red Zebra Danio - Dan...

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Main Author: Ahmad Tarmizi, Nur Farhanum
Format: Project Paper Report
Language:English
Published: 2018
Online Access:http://psasir.upm.edu.my/id/eprint/85783/1/FP%202018%2023%20IR.pdf
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author Ahmad Tarmizi, Nur Farhanum
author_facet Ahmad Tarmizi, Nur Farhanum
author_sort Ahmad Tarmizi, Nur Farhanum
collection UPM
description The aim of this study was to determine the percentage of differential types of leukocytes in commercial ornamental fish (Green Tiger Barb - Puntigrus tetrazona, Rosy Tetra - Hyphessobrycon rosaceus, Rosy Barb - Pethia conchonius, Red Eye Tetra - Moenkhausia sanctaefilomenae and Red Zebra Danio - Danio rerio) and G:L (granulocyte to lymphocyte) ratio as stress index of these species. This study examined 20 samples (four specimens for each species) from the controlled conditions in good health condition. The leukocytes stained by May-Grunwald Giemsa solution and were classified to four types; lymphocytes, granulocytes, monocytes and thrombocytes. Lymphocytes were the most abundant leukocytes found in the peripheral blood of all tested species, in which the lowest percentage lymphocytes was recorded in Rosy Tetra (81.74±3.27) while the highest value was Red Eye Tetra (94.57±1.32). The G:L ratio of the tested species were lower than 0.5, where Rosy Tetra recorded the highest G:L ratio among the tested (0.120±0.021), meanwhile Red Eye Tetra was the lowest (0.007±0.003). The current result provides the reference values and background for the future experiments. Any increment from the G:L ratio of the respective species reported in this study can be an indication of stress.
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spelling upm.eprints-857832021-06-21T06:22:17Z http://psasir.upm.edu.my/id/eprint/85783/ Differential cell counts in 5 commercial ornamental fishes Ahmad Tarmizi, Nur Farhanum The aim of this study was to determine the percentage of differential types of leukocytes in commercial ornamental fish (Green Tiger Barb - Puntigrus tetrazona, Rosy Tetra - Hyphessobrycon rosaceus, Rosy Barb - Pethia conchonius, Red Eye Tetra - Moenkhausia sanctaefilomenae and Red Zebra Danio - Danio rerio) and G:L (granulocyte to lymphocyte) ratio as stress index of these species. This study examined 20 samples (four specimens for each species) from the controlled conditions in good health condition. The leukocytes stained by May-Grunwald Giemsa solution and were classified to four types; lymphocytes, granulocytes, monocytes and thrombocytes. Lymphocytes were the most abundant leukocytes found in the peripheral blood of all tested species, in which the lowest percentage lymphocytes was recorded in Rosy Tetra (81.74±3.27) while the highest value was Red Eye Tetra (94.57±1.32). The G:L ratio of the tested species were lower than 0.5, where Rosy Tetra recorded the highest G:L ratio among the tested (0.120±0.021), meanwhile Red Eye Tetra was the lowest (0.007±0.003). The current result provides the reference values and background for the future experiments. Any increment from the G:L ratio of the respective species reported in this study can be an indication of stress. 2018 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/85783/1/FP%202018%2023%20IR.pdf Ahmad Tarmizi, Nur Farhanum (2018) Differential cell counts in 5 commercial ornamental fishes. [Project Paper Report]
spellingShingle Ahmad Tarmizi, Nur Farhanum
Differential cell counts in 5 commercial ornamental fishes
title Differential cell counts in 5 commercial ornamental fishes
title_full Differential cell counts in 5 commercial ornamental fishes
title_fullStr Differential cell counts in 5 commercial ornamental fishes
title_full_unstemmed Differential cell counts in 5 commercial ornamental fishes
title_short Differential cell counts in 5 commercial ornamental fishes
title_sort differential cell counts in 5 commercial ornamental fishes
url http://psasir.upm.edu.my/id/eprint/85783/1/FP%202018%2023%20IR.pdf
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