Sensitive and selective detection of DNA fragments associated with Ganoderma boninense by DNA-nanoparticle conjugate hybridisation
A colourimetric assay for the detection of DNA fragments associated with the oil palm pathogen Ganoderma boninense and other fungi DNA is reported. The assay is based on the aggregation of DNA-nanoparticle conjugates in the presence of complementary DNA from the target organism. Here, various design...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Springer New York LLC
2020
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| _version_ | 1796981685288435712 |
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| author | Ekta Rani Mohshim, Siti Akhtar Yusof, Nor Hidayat Ahmad, Muhammad Zamharir Goodacre, Royston Alang Ahmad, Shahrul Ainliah Wong, Lu Shin |
| author_facet | Ekta Rani Mohshim, Siti Akhtar Yusof, Nor Hidayat Ahmad, Muhammad Zamharir Goodacre, Royston Alang Ahmad, Shahrul Ainliah Wong, Lu Shin |
| author_sort | Ekta Rani |
| collection | UPM |
| description | A colourimetric assay for the detection of DNA fragments associated with the oil palm pathogen Ganoderma boninense and other fungi DNA is reported. The assay is based on the aggregation of DNA-nanoparticle conjugates in the presence of complementary DNA from the target organism. Here, various designs of DNA-nanoparticle conjugates were evaluated, and it was found that the best design gave a visually observable colour change with as little as 2 pmol of double-stranded DNA analyte even in the presence of a large excess of a mixture of non-complementary DNA. Overall, this label-free system is rapid, sensitive, selective, simple in design, and easy to carry out. It does not require specialist equipment or specialist training for the interpretation of the results, and therefore has the potential to be deployed for agricultural diagnostics in the field. |
| first_indexed | 2024-03-06T10:41:15Z |
| format | Article |
| id | upm.eprints-85794 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:41:15Z |
| publishDate | 2020 |
| publisher | Springer New York LLC |
| record_format | dspace |
| spelling | upm.eprints-857942023-09-05T08:51:17Z http://psasir.upm.edu.my/id/eprint/85794/ Sensitive and selective detection of DNA fragments associated with Ganoderma boninense by DNA-nanoparticle conjugate hybridisation Ekta Rani Mohshim, Siti Akhtar Yusof, Nor Hidayat Ahmad, Muhammad Zamharir Goodacre, Royston Alang Ahmad, Shahrul Ainliah Wong, Lu Shin A colourimetric assay for the detection of DNA fragments associated with the oil palm pathogen Ganoderma boninense and other fungi DNA is reported. The assay is based on the aggregation of DNA-nanoparticle conjugates in the presence of complementary DNA from the target organism. Here, various designs of DNA-nanoparticle conjugates were evaluated, and it was found that the best design gave a visually observable colour change with as little as 2 pmol of double-stranded DNA analyte even in the presence of a large excess of a mixture of non-complementary DNA. Overall, this label-free system is rapid, sensitive, selective, simple in design, and easy to carry out. It does not require specialist equipment or specialist training for the interpretation of the results, and therefore has the potential to be deployed for agricultural diagnostics in the field. Springer New York LLC 2020 Article PeerReviewed Ekta Rani and Mohshim, Siti Akhtar and Yusof, Nor Hidayat and Ahmad, Muhammad Zamharir and Goodacre, Royston and Alang Ahmad, Shahrul Ainliah and Wong, Lu Shin (2020) Sensitive and selective detection of DNA fragments associated with Ganoderma boninense by DNA-nanoparticle conjugate hybridisation. Journal of Materials Science, 55. 14965 - 14979. ISSN 0022-2461; ESSN: 1573-4803 https://link.springer.com/article/10.1007/s10853-020-05058-8 10.1007/s10853-020-05058-8 |
| spellingShingle | Ekta Rani Mohshim, Siti Akhtar Yusof, Nor Hidayat Ahmad, Muhammad Zamharir Goodacre, Royston Alang Ahmad, Shahrul Ainliah Wong, Lu Shin Sensitive and selective detection of DNA fragments associated with Ganoderma boninense by DNA-nanoparticle conjugate hybridisation |
| title | Sensitive and selective detection of DNA fragments associated with Ganoderma boninense by DNA-nanoparticle conjugate hybridisation |
| title_full | Sensitive and selective detection of DNA fragments associated with Ganoderma boninense by DNA-nanoparticle conjugate hybridisation |
| title_fullStr | Sensitive and selective detection of DNA fragments associated with Ganoderma boninense by DNA-nanoparticle conjugate hybridisation |
| title_full_unstemmed | Sensitive and selective detection of DNA fragments associated with Ganoderma boninense by DNA-nanoparticle conjugate hybridisation |
| title_short | Sensitive and selective detection of DNA fragments associated with Ganoderma boninense by DNA-nanoparticle conjugate hybridisation |
| title_sort | sensitive and selective detection of dna fragments associated with ganoderma boninense by dna nanoparticle conjugate hybridisation |
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