Sensitive detection of dengue virus type 2 E-proteins signals using self-assembled monolayers/reduced graphene oxide-PAMAM dendrimer thin film-SPR optical sensor
In this work, sensitive detection of dengue virus type 2 E-proteins (DENV-2 E-proteins) was performed in the range of 0.08 pM to 0.5 pM. The successful DENV detection at very low concentration is a matter of concern for targeting the early detection after the onset of dengue symptoms. Here, we devel...
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| Format: | Article |
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Nature Publishing Group
2020
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| author | Sheh Omar, Nur Alia Fen, Yap Wing Abdullah, Jaafar Mustapha Kamil, Yasmin Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Sadrolhosseini, Amir Reza Mahdi, Mohd Adzir |
| author_facet | Sheh Omar, Nur Alia Fen, Yap Wing Abdullah, Jaafar Mustapha Kamil, Yasmin Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Sadrolhosseini, Amir Reza Mahdi, Mohd Adzir |
| author_sort | Sheh Omar, Nur Alia |
| collection | UPM |
| description | In this work, sensitive detection of dengue virus type 2 E-proteins (DENV-2 E-proteins) was performed in the range of 0.08 pM to 0.5 pM. The successful DENV detection at very low concentration is a matter of concern for targeting the early detection after the onset of dengue symptoms. Here, we developed a SPR sensor based on self-assembled monolayer/reduced graphene oxide-polyamidoamine dendrimer (SAM/NH2rGO/PAMAM) thin film to detect DENV-2 E-proteins. Surface characterizations involving X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) confirms the incorporation of NH2rGO-PAMAM nanoparticles in the prepared sensor films. The specificity, sensitivity, binding affinity, and selectivity of the SPR sensor were then evaluated. Results indicated that the variation of the sensing layer due to different spin speed, time incubation, and concentration provided a better interaction between the analyte and sensing layer. The linear dependence of the SPR sensor showed good linearity (R2 = 0.92) with the lowest detection of 0.08 pM DENV-2 E-proteins. By using the Langmuir model, the equilibrium association constant was obtained at very high value of 6.6844 TM−1 (R2 = 0.99). High selectivity of the SPR sensor towards DENV-2 E-proteins was achieved in the presence of other competitors. |
| first_indexed | 2024-03-06T10:41:15Z |
| format | Article |
| id | upm.eprints-85795 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:41:15Z |
| publishDate | 2020 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | upm.eprints-857952023-09-06T08:40:43Z http://psasir.upm.edu.my/id/eprint/85795/ Sensitive detection of dengue virus type 2 E-proteins signals using self-assembled monolayers/reduced graphene oxide-PAMAM dendrimer thin film-SPR optical sensor Sheh Omar, Nur Alia Fen, Yap Wing Abdullah, Jaafar Mustapha Kamil, Yasmin Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Sadrolhosseini, Amir Reza Mahdi, Mohd Adzir In this work, sensitive detection of dengue virus type 2 E-proteins (DENV-2 E-proteins) was performed in the range of 0.08 pM to 0.5 pM. The successful DENV detection at very low concentration is a matter of concern for targeting the early detection after the onset of dengue symptoms. Here, we developed a SPR sensor based on self-assembled monolayer/reduced graphene oxide-polyamidoamine dendrimer (SAM/NH2rGO/PAMAM) thin film to detect DENV-2 E-proteins. Surface characterizations involving X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) confirms the incorporation of NH2rGO-PAMAM nanoparticles in the prepared sensor films. The specificity, sensitivity, binding affinity, and selectivity of the SPR sensor were then evaluated. Results indicated that the variation of the sensing layer due to different spin speed, time incubation, and concentration provided a better interaction between the analyte and sensing layer. The linear dependence of the SPR sensor showed good linearity (R2 = 0.92) with the lowest detection of 0.08 pM DENV-2 E-proteins. By using the Langmuir model, the equilibrium association constant was obtained at very high value of 6.6844 TM−1 (R2 = 0.99). High selectivity of the SPR sensor towards DENV-2 E-proteins was achieved in the presence of other competitors. Nature Publishing Group 2020 Article PeerReviewed Sheh Omar, Nur Alia and Fen, Yap Wing and Abdullah, Jaafar and Mustapha Kamil, Yasmin and Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim and Sadrolhosseini, Amir Reza and Mahdi, Mohd Adzir (2020) Sensitive detection of dengue virus type 2 E-proteins signals using self-assembled monolayers/reduced graphene oxide-PAMAM dendrimer thin film-SPR optical sensor. Scientific Reports, 10. art. no. 2374. pp. 1-15. ISSN 2045-2322 https://www.nature.com/articles/s41598-020-59388-3 10.1038/s41598-020-59388-3 |
| spellingShingle | Sheh Omar, Nur Alia Fen, Yap Wing Abdullah, Jaafar Mustapha Kamil, Yasmin Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Sadrolhosseini, Amir Reza Mahdi, Mohd Adzir Sensitive detection of dengue virus type 2 E-proteins signals using self-assembled monolayers/reduced graphene oxide-PAMAM dendrimer thin film-SPR optical sensor |
| title | Sensitive detection of dengue virus type 2 E-proteins signals using self-assembled monolayers/reduced graphene oxide-PAMAM dendrimer thin film-SPR optical sensor |
| title_full | Sensitive detection of dengue virus type 2 E-proteins signals using self-assembled monolayers/reduced graphene oxide-PAMAM dendrimer thin film-SPR optical sensor |
| title_fullStr | Sensitive detection of dengue virus type 2 E-proteins signals using self-assembled monolayers/reduced graphene oxide-PAMAM dendrimer thin film-SPR optical sensor |
| title_full_unstemmed | Sensitive detection of dengue virus type 2 E-proteins signals using self-assembled monolayers/reduced graphene oxide-PAMAM dendrimer thin film-SPR optical sensor |
| title_short | Sensitive detection of dengue virus type 2 E-proteins signals using self-assembled monolayers/reduced graphene oxide-PAMAM dendrimer thin film-SPR optical sensor |
| title_sort | sensitive detection of dengue virus type 2 e proteins signals using self assembled monolayers reduced graphene oxide pamam dendrimer thin film spr optical sensor |
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