Simultaneous pretreatment and saccharification of oil palm empty fruit bunch using laccase-cellulase cocktail
Oil palm empty fruit bunch (OPEFB) can be categorized as one of the toughest lignocellulosic biomass materials to be degraded naturally due to its complexity in structure. A new biological pretreatment approach of utilizing laccase-cellulase cocktail to simultaneously pretreat and saccharify OPEFB f...
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| Format: | Article |
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Elsevier BV
2020
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| _version_ | 1825952195553525760 |
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| author | Masran, Ruqayyah Kamal Bahrin, Ezyana Ibrahim, Mohamad Faizal Phang, Lai Yee Abd Aziz, Suraini |
| author_facet | Masran, Ruqayyah Kamal Bahrin, Ezyana Ibrahim, Mohamad Faizal Phang, Lai Yee Abd Aziz, Suraini |
| author_sort | Masran, Ruqayyah |
| collection | UPM |
| description | Oil palm empty fruit bunch (OPEFB) can be categorized as one of the toughest lignocellulosic biomass materials to be degraded naturally due to its complexity in structure. A new biological pretreatment approach of utilizing laccase-cellulase cocktail to simultaneously pretreat and saccharify OPEFB for reducing sugars production was presented in this study. Combination of laccase and cellulase has shown to exhibit minimum inhibition at 5% and 8%, respectively. This simultaneous pretreatment and saccharification of OPEFB using laccase-cellulase cocktail comprised of laccase (25–105 U/g) and Acremonium cellulase (10–30 FPU/g) was carried out at 50 °C for 72 h. As a result, the laccase-cellulase cocktail at a ratio of 45 U/g:25 FPU/g successfully produced 10.90 g/L of reducing sugars and 32.62% of hydrolysis yield, without the need of substrate washing step after pretreatment, in which it shortens the time from a total of 144 h–72 h and reduces the utilization of extra vessels. |
| first_indexed | 2024-03-06T10:41:20Z |
| format | Article |
| id | upm.eprints-85824 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:41:20Z |
| publishDate | 2020 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | upm.eprints-858242023-10-02T00:39:58Z http://psasir.upm.edu.my/id/eprint/85824/ Simultaneous pretreatment and saccharification of oil palm empty fruit bunch using laccase-cellulase cocktail Masran, Ruqayyah Kamal Bahrin, Ezyana Ibrahim, Mohamad Faizal Phang, Lai Yee Abd Aziz, Suraini Oil palm empty fruit bunch (OPEFB) can be categorized as one of the toughest lignocellulosic biomass materials to be degraded naturally due to its complexity in structure. A new biological pretreatment approach of utilizing laccase-cellulase cocktail to simultaneously pretreat and saccharify OPEFB for reducing sugars production was presented in this study. Combination of laccase and cellulase has shown to exhibit minimum inhibition at 5% and 8%, respectively. This simultaneous pretreatment and saccharification of OPEFB using laccase-cellulase cocktail comprised of laccase (25–105 U/g) and Acremonium cellulase (10–30 FPU/g) was carried out at 50 °C for 72 h. As a result, the laccase-cellulase cocktail at a ratio of 45 U/g:25 FPU/g successfully produced 10.90 g/L of reducing sugars and 32.62% of hydrolysis yield, without the need of substrate washing step after pretreatment, in which it shortens the time from a total of 144 h–72 h and reduces the utilization of extra vessels. Elsevier BV 2020 Article PeerReviewed Masran, Ruqayyah and Kamal Bahrin, Ezyana and Ibrahim, Mohamad Faizal and Phang, Lai Yee and Abd Aziz, Suraini (2020) Simultaneous pretreatment and saccharification of oil palm empty fruit bunch using laccase-cellulase cocktail. Biocatalysis and Agricultural Biotechnology, 29 (1). pp. 1-6. ISSN 1878-8181 https://www.sciencedirect.com/science/article/pii/S1878818120308768 10.1016/j.bcab.2020.101824 |
| spellingShingle | Masran, Ruqayyah Kamal Bahrin, Ezyana Ibrahim, Mohamad Faizal Phang, Lai Yee Abd Aziz, Suraini Simultaneous pretreatment and saccharification of oil palm empty fruit bunch using laccase-cellulase cocktail |
| title | Simultaneous pretreatment and saccharification of oil palm empty fruit bunch using laccase-cellulase cocktail |
| title_full | Simultaneous pretreatment and saccharification of oil palm empty fruit bunch using laccase-cellulase cocktail |
| title_fullStr | Simultaneous pretreatment and saccharification of oil palm empty fruit bunch using laccase-cellulase cocktail |
| title_full_unstemmed | Simultaneous pretreatment and saccharification of oil palm empty fruit bunch using laccase-cellulase cocktail |
| title_short | Simultaneous pretreatment and saccharification of oil palm empty fruit bunch using laccase-cellulase cocktail |
| title_sort | simultaneous pretreatment and saccharification of oil palm empty fruit bunch using laccase cellulase cocktail |
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