Stevia: limiting cholesterol synthesis in Hep-G2 cells
As of today, no literature has been reported on the efficacy of stevia on lipid regulations conducted in vitro. Thus, the current study was focusing on the potential of Stevia rebaudiana bertoni as an anti-hypercholesterolemia substitute in limiting the de novo cholesterol synthesis in Hep-G2 cell...
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| Format: | Article |
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Malaysian Society for Molecular Biology and Biotechnology
2020
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| _version_ | 1825952209566695424 |
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| author | Ilias, Amirul Nazhan Hamzah, Hazilawati Ismail, Intan Safinar Ajat, Mokrish |
| author_facet | Ilias, Amirul Nazhan Hamzah, Hazilawati Ismail, Intan Safinar Ajat, Mokrish |
| author_sort | Ilias, Amirul Nazhan |
| collection | UPM |
| description | As of today, no literature has been reported on the efficacy of stevia on lipid regulations
conducted in vitro. Thus, the current study was focusing on the potential of Stevia rebaudiana bertoni as an
anti-hypercholesterolemia substitute in limiting the de novo cholesterol synthesis in Hep-G2 cell line. The
cytotoxicity and lipid internalization effects of stevia on Hep-G2 cells were assessed quantitatively and
qualitatively in this study. As evaluated by MTT assay, commercialized stevia (0.5-20.0 mg/ml) and
stevioside (1.0-10 µM) inhibited Hep-G2 cells viability in a dose-dependent manner for 24 hours. IC50 was
detected at 8.68 mg/ml (commercialized stevia) and 10.91 µM (stevioside). From the assay, suitable
concentrations were chosen to study the effect of stevia on cholesterol internalization in Hep-G2 cells
supplemented with exogenous lipids. Cholesterol quantification assay revealed that high concentration
commercialized stevia and stevioside promoted significant cholesterol internalized in Hep-G2 cells as
compared to simvastatin. Finally, immunofluorescent microscopy assessment was done to qualitatively
observe the formation of lipid droplets and low-density lipoprotein receptor in relation to total cholesterol
extracted. The microphotographs of immunofluorescent microscopy were in parallel to results obtained
from the cholesterol quantification assay which further revealed the effect of stevia as a potential antihypercholesterolemia agent. |
| first_indexed | 2024-03-06T10:41:31Z |
| format | Article |
| id | upm.eprints-85917 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:41:31Z |
| publishDate | 2020 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| record_format | dspace |
| spelling | upm.eprints-859172023-10-24T08:38:19Z http://psasir.upm.edu.my/id/eprint/85917/ Stevia: limiting cholesterol synthesis in Hep-G2 cells Ilias, Amirul Nazhan Hamzah, Hazilawati Ismail, Intan Safinar Ajat, Mokrish As of today, no literature has been reported on the efficacy of stevia on lipid regulations conducted in vitro. Thus, the current study was focusing on the potential of Stevia rebaudiana bertoni as an anti-hypercholesterolemia substitute in limiting the de novo cholesterol synthesis in Hep-G2 cell line. The cytotoxicity and lipid internalization effects of stevia on Hep-G2 cells were assessed quantitatively and qualitatively in this study. As evaluated by MTT assay, commercialized stevia (0.5-20.0 mg/ml) and stevioside (1.0-10 µM) inhibited Hep-G2 cells viability in a dose-dependent manner for 24 hours. IC50 was detected at 8.68 mg/ml (commercialized stevia) and 10.91 µM (stevioside). From the assay, suitable concentrations were chosen to study the effect of stevia on cholesterol internalization in Hep-G2 cells supplemented with exogenous lipids. Cholesterol quantification assay revealed that high concentration commercialized stevia and stevioside promoted significant cholesterol internalized in Hep-G2 cells as compared to simvastatin. Finally, immunofluorescent microscopy assessment was done to qualitatively observe the formation of lipid droplets and low-density lipoprotein receptor in relation to total cholesterol extracted. The microphotographs of immunofluorescent microscopy were in parallel to results obtained from the cholesterol quantification assay which further revealed the effect of stevia as a potential antihypercholesterolemia agent. Malaysian Society for Molecular Biology and Biotechnology 2020 Article PeerReviewed Ilias, Amirul Nazhan and Hamzah, Hazilawati and Ismail, Intan Safinar and Ajat, Mokrish (2020) Stevia: limiting cholesterol synthesis in Hep-G2 cells. Asia-Pacific Journal of Molecular Biology and Biotechnology, 28 (1). 111 - 119. ISSN 0128-7451; ESSN: 2672-7277 http://www.msmbb.my/index.php/archive-issues/18-apjmbb/451-archive-issue-28 10.35118/apjmbb.2020.028.1.11 |
| spellingShingle | Ilias, Amirul Nazhan Hamzah, Hazilawati Ismail, Intan Safinar Ajat, Mokrish Stevia: limiting cholesterol synthesis in Hep-G2 cells |
| title | Stevia: limiting cholesterol synthesis in Hep-G2 cells |
| title_full | Stevia: limiting cholesterol synthesis in Hep-G2 cells |
| title_fullStr | Stevia: limiting cholesterol synthesis in Hep-G2 cells |
| title_full_unstemmed | Stevia: limiting cholesterol synthesis in Hep-G2 cells |
| title_short | Stevia: limiting cholesterol synthesis in Hep-G2 cells |
| title_sort | stevia limiting cholesterol synthesis in hep g2 cells |
| work_keys_str_mv | AT iliasamirulnazhan stevialimitingcholesterolsynthesisinhepg2cells AT hamzahhazilawati stevialimitingcholesterolsynthesisinhepg2cells AT ismailintansafinar stevialimitingcholesterolsynthesisinhepg2cells AT ajatmokrish stevialimitingcholesterolsynthesisinhepg2cells |