Thermostability and Protein Studies of Newcastle Disease Virus
The heat stability of four strains of Newcastle disease virus (NDV) belonging to three different pathotypes were studied. The strains were the lentogenic V4 and its heat stable variant V4-UPM, the mesogenic S strain and the velogenic strain AF2240. Analyses of their haemagglutination and neuram...
| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
1995
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| Online Access: | http://psasir.upm.edu.my/id/eprint/8598/1/FSAS_1995_4_A.pdf |
| _version_ | 1796967561977397248 |
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| author | Hassan, Zuridah |
| author_facet | Hassan, Zuridah |
| author_sort | Hassan, Zuridah |
| collection | UPM |
| description | The heat stability of four strains of Newcastle
disease virus (NDV) belonging to three different
pathotypes were studied. The strains were the
lentogenic V4 and its heat stable variant V4-UPM, the
mesogenic S strain and the velogenic strain AF2240.
Analyses of their haemagglutination and neuraminidase
activities (which are the functions of the HN protein)
and the hemolytic activities at various temperatures
showed that strains AF2240, V4 and V4 -UPM were heat
stable compared to strain S.
There were no differences observed in the
mobilities of the various NDV proteins on sodium dodecyl sulphate-polyacrylamide gel electrophoretic
(SDS-PAGE) studies. However, analysis of the various
peptides with Staphylococcus aureus protease showed
that the digested HN proteins of strain V4-UPM was
different from the strains V4, AF2240 and S. The
peptide analysis was repeated using Pseudomonas fragi
Endoproteinase Asp-N and Lysobacter enzymogenes Lys-C
and found to be similar except in strain V4-UPM.
These proteins were further analysed by the two dimensional
polyacrylamide gel electrophoresis (2DPAGE).
The gels were then Western blotted and protein
spots were identified using HN,NP and Fi Mabs and then
analysed by the UVP GDS Gel Documentation System
(United Kingdom). It was observed that in the heat
stable V4 strain the isoelectric point (pI) of the HN
protein was in the acidic region, strains V4-UPM and
AF2240 in the neutral/weak basic regions and in the
thermolabile strain s, the HN protein was shifted to
the basic end of the isoelectric focussing run. The pI
changes in the NP protein was seen in strain S only.
The F protein was at the basic region for all strain
except strain V4-UPM. In strain S it was seen that the
HN, NP and F proteins were in the basic region and
this basic pI could be responsible for the different
biological characteristics seen in the thermostable
strains compared to the thermosensitive strain. |
| first_indexed | 2024-03-06T07:15:41Z |
| format | Thesis |
| id | upm.eprints-8598 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:15:41Z |
| publishDate | 1995 |
| record_format | dspace |
| spelling | upm.eprints-85982010-12-02T09:42:00Z http://psasir.upm.edu.my/id/eprint/8598/ Thermostability and Protein Studies of Newcastle Disease Virus Hassan, Zuridah The heat stability of four strains of Newcastle disease virus (NDV) belonging to three different pathotypes were studied. The strains were the lentogenic V4 and its heat stable variant V4-UPM, the mesogenic S strain and the velogenic strain AF2240. Analyses of their haemagglutination and neuraminidase activities (which are the functions of the HN protein) and the hemolytic activities at various temperatures showed that strains AF2240, V4 and V4 -UPM were heat stable compared to strain S. There were no differences observed in the mobilities of the various NDV proteins on sodium dodecyl sulphate-polyacrylamide gel electrophoretic (SDS-PAGE) studies. However, analysis of the various peptides with Staphylococcus aureus protease showed that the digested HN proteins of strain V4-UPM was different from the strains V4, AF2240 and S. The peptide analysis was repeated using Pseudomonas fragi Endoproteinase Asp-N and Lysobacter enzymogenes Lys-C and found to be similar except in strain V4-UPM. These proteins were further analysed by the two dimensional polyacrylamide gel electrophoresis (2DPAGE). The gels were then Western blotted and protein spots were identified using HN,NP and Fi Mabs and then analysed by the UVP GDS Gel Documentation System (United Kingdom). It was observed that in the heat stable V4 strain the isoelectric point (pI) of the HN protein was in the acidic region, strains V4-UPM and AF2240 in the neutral/weak basic regions and in the thermolabile strain s, the HN protein was shifted to the basic end of the isoelectric focussing run. The pI changes in the NP protein was seen in strain S only. The F protein was at the basic region for all strain except strain V4-UPM. In strain S it was seen that the HN, NP and F proteins were in the basic region and this basic pI could be responsible for the different biological characteristics seen in the thermostable strains compared to the thermosensitive strain. 1995 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/8598/1/FSAS_1995_4_A.pdf Hassan, Zuridah (1995) Thermostability and Protein Studies of Newcastle Disease Virus. Masters thesis, Universiti Pertanian Malaysia. English |
| spellingShingle | Hassan, Zuridah Thermostability and Protein Studies of Newcastle Disease Virus |
| title | Thermostability and Protein Studies of Newcastle Disease Virus |
| title_full | Thermostability and Protein Studies of Newcastle Disease Virus |
| title_fullStr | Thermostability and Protein Studies of Newcastle Disease Virus |
| title_full_unstemmed | Thermostability and Protein Studies of Newcastle Disease Virus |
| title_short | Thermostability and Protein Studies of Newcastle Disease Virus |
| title_sort | thermostability and protein studies of newcastle disease virus |
| url | http://psasir.upm.edu.my/id/eprint/8598/1/FSAS_1995_4_A.pdf |
| work_keys_str_mv | AT hassanzuridah thermostabilityandproteinstudiesofnewcastlediseasevirus |