Long-term monitoring of donor xenogeneic testis tissue grafts and cell implants in recipient mice using ultrasound biomicroscopy
Testis tissue xenografting and testis cell aggregate implantation from various donor species into recipient mice are novel models for the study and manipulation of testis formation and function in target species. Thus far, the analysis of such studies has been limited to surgical or post-mortem retr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86595/1/Long-term%20monitoring%20of%20donor.pdf |
| _version_ | 1796981723498545152 |
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| author | Fayaz, Mohammad Amin Awang Junaidi, Awang Hazmi Singh, Jaswant Honaramooz, Ali |
| author_facet | Fayaz, Mohammad Amin Awang Junaidi, Awang Hazmi Singh, Jaswant Honaramooz, Ali |
| author_sort | Fayaz, Mohammad Amin |
| collection | UPM |
| description | Testis tissue xenografting and testis cell aggregate implantation from various donor species into recipient mice are novel models for the study and manipulation of testis formation and function in target species. Thus far, the analysis of such studies has been limited to surgical or post-mortem retrieval of samples. Here we used ultrasound biomicroscopy (UBM) to monitor the development of neonatal porcine testis grafts and implants in host mice for 24 wk, and to correlate UBM and (immuno)histologic changes. This led to long-term visualization of gradual changes in volume, dimension and structure of grafts and implants; detection of a 4 wk developmental gap between grafts and implants; and revelation of differences in implant development depending on the craniocaudal site of implantation on the back of host mice. Our data support the reliability and precision of UBM for longitudinal study of transplants, which eliminates the need for frequent surgical sampling. |
| first_indexed | 2024-03-06T10:41:49Z |
| format | Article |
| id | upm.eprints-86595 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:41:49Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-865952021-09-26T23:08:50Z http://psasir.upm.edu.my/id/eprint/86595/ Long-term monitoring of donor xenogeneic testis tissue grafts and cell implants in recipient mice using ultrasound biomicroscopy Fayaz, Mohammad Amin Awang Junaidi, Awang Hazmi Singh, Jaswant Honaramooz, Ali Testis tissue xenografting and testis cell aggregate implantation from various donor species into recipient mice are novel models for the study and manipulation of testis formation and function in target species. Thus far, the analysis of such studies has been limited to surgical or post-mortem retrieval of samples. Here we used ultrasound biomicroscopy (UBM) to monitor the development of neonatal porcine testis grafts and implants in host mice for 24 wk, and to correlate UBM and (immuno)histologic changes. This led to long-term visualization of gradual changes in volume, dimension and structure of grafts and implants; detection of a 4 wk developmental gap between grafts and implants; and revelation of differences in implant development depending on the craniocaudal site of implantation on the back of host mice. Our data support the reliability and precision of UBM for longitudinal study of transplants, which eliminates the need for frequent surgical sampling. Elsevier 2020-11-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86595/1/Long-term%20monitoring%20of%20donor.pdf Fayaz, Mohammad Amin and Awang Junaidi, Awang Hazmi and Singh, Jaswant and Honaramooz, Ali (2020) Long-term monitoring of donor xenogeneic testis tissue grafts and cell implants in recipient mice using ultrasound biomicroscopy. Ultrasound in Medicine and Biology, 46 (11). 3088 - 3103. ISSN 0301-5629; ESSN: 1879-291X https://www.umbjournal.org/article/S0301-5629(20)30306-9/fulltext 10.1016/j.ultrasmedbio.2020.07.010 |
| spellingShingle | Fayaz, Mohammad Amin Awang Junaidi, Awang Hazmi Singh, Jaswant Honaramooz, Ali Long-term monitoring of donor xenogeneic testis tissue grafts and cell implants in recipient mice using ultrasound biomicroscopy |
| title | Long-term monitoring of donor xenogeneic testis tissue grafts and cell implants in recipient mice using ultrasound biomicroscopy |
| title_full | Long-term monitoring of donor xenogeneic testis tissue grafts and cell implants in recipient mice using ultrasound biomicroscopy |
| title_fullStr | Long-term monitoring of donor xenogeneic testis tissue grafts and cell implants in recipient mice using ultrasound biomicroscopy |
| title_full_unstemmed | Long-term monitoring of donor xenogeneic testis tissue grafts and cell implants in recipient mice using ultrasound biomicroscopy |
| title_short | Long-term monitoring of donor xenogeneic testis tissue grafts and cell implants in recipient mice using ultrasound biomicroscopy |
| title_sort | long term monitoring of donor xenogeneic testis tissue grafts and cell implants in recipient mice using ultrasound biomicroscopy |
| url | http://psasir.upm.edu.my/id/eprint/86595/1/Long-term%20monitoring%20of%20donor.pdf |
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