Mass spectrometry-based metabolomics combined with quantitative analysis of the microalgal diatom (Chaetoceros calcitrans)
Although many metabolomics studies of higher land plant species have been conducted, similar studies of lower nonland plant species, which include microalgae, are still developing. The present study represents an attempt to characterize the metabolic profile of a microalgal diatom Chaetoceros calcit...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86651/1/Mass%20spectrometry-based%20.pdf |
| _version_ | 1796981733480988672 |
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| author | Azizan, Awanis M., Maulidiani R., Rudiyanto Shaari, Khozirah Ismail, Intan Safinar Nagao, Norio Abas, Faridah |
| author_facet | Azizan, Awanis M., Maulidiani R., Rudiyanto Shaari, Khozirah Ismail, Intan Safinar Nagao, Norio Abas, Faridah |
| author_sort | Azizan, Awanis |
| collection | UPM |
| description | Although many metabolomics studies of higher land plant species have been conducted, similar studies of lower nonland plant species, which include microalgae, are still developing. The present study represents an attempt to characterize the metabolic profile of a microalgal diatom Chaetoceros calcitrans, by applying high-resolution mass spectrometry detection, via Q-ExactiveTM Plus Orbitrap mass spectrometry. The results showed that 54 metabolites of various classes were tentatively identified. Experimentally, the chloroform and acetone extracts were clearly distinguished from other solvent extracts in chemometric regression analysis using PLS, showing the differences in the C. calcitrans metabolome between the groups. In addition, specific metabolites were evaluated, which supported the finding of antioxidant and anti-inflammatory activities. This study also provides data on the quantitative analysis of four carotenoids based on the identification results. Therefore, these findings could serve as a reliable tool for identifying and quantifying the metabolome that could reflect the metabolic activities of C. calcitrans. |
| first_indexed | 2024-03-06T10:41:58Z |
| format | Article |
| id | upm.eprints-86651 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:41:58Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-866512021-10-17T21:54:45Z http://psasir.upm.edu.my/id/eprint/86651/ Mass spectrometry-based metabolomics combined with quantitative analysis of the microalgal diatom (Chaetoceros calcitrans) Azizan, Awanis M., Maulidiani R., Rudiyanto Shaari, Khozirah Ismail, Intan Safinar Nagao, Norio Abas, Faridah Although many metabolomics studies of higher land plant species have been conducted, similar studies of lower nonland plant species, which include microalgae, are still developing. The present study represents an attempt to characterize the metabolic profile of a microalgal diatom Chaetoceros calcitrans, by applying high-resolution mass spectrometry detection, via Q-ExactiveTM Plus Orbitrap mass spectrometry. The results showed that 54 metabolites of various classes were tentatively identified. Experimentally, the chloroform and acetone extracts were clearly distinguished from other solvent extracts in chemometric regression analysis using PLS, showing the differences in the C. calcitrans metabolome between the groups. In addition, specific metabolites were evaluated, which supported the finding of antioxidant and anti-inflammatory activities. This study also provides data on the quantitative analysis of four carotenoids based on the identification results. Therefore, these findings could serve as a reliable tool for identifying and quantifying the metabolome that could reflect the metabolic activities of C. calcitrans. Multidisciplinary Digital Publishing Institute 2020-07-30 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86651/1/Mass%20spectrometry-based%20.pdf Azizan, Awanis and M., Maulidiani and R., Rudiyanto and Shaari, Khozirah and Ismail, Intan Safinar and Nagao, Norio and Abas, Faridah (2020) Mass spectrometry-based metabolomics combined with quantitative analysis of the microalgal diatom (Chaetoceros calcitrans). Marine Drugs, 18 (8). pp. 1-22. ISSN 1660-3397 https://www.mdpi.com/1660-3397/18/8/403 10.3390/md18080403 |
| spellingShingle | Azizan, Awanis M., Maulidiani R., Rudiyanto Shaari, Khozirah Ismail, Intan Safinar Nagao, Norio Abas, Faridah Mass spectrometry-based metabolomics combined with quantitative analysis of the microalgal diatom (Chaetoceros calcitrans) |
| title | Mass spectrometry-based metabolomics combined with quantitative analysis of the microalgal diatom (Chaetoceros calcitrans) |
| title_full | Mass spectrometry-based metabolomics combined with quantitative analysis of the microalgal diatom (Chaetoceros calcitrans) |
| title_fullStr | Mass spectrometry-based metabolomics combined with quantitative analysis of the microalgal diatom (Chaetoceros calcitrans) |
| title_full_unstemmed | Mass spectrometry-based metabolomics combined with quantitative analysis of the microalgal diatom (Chaetoceros calcitrans) |
| title_short | Mass spectrometry-based metabolomics combined with quantitative analysis of the microalgal diatom (Chaetoceros calcitrans) |
| title_sort | mass spectrometry based metabolomics combined with quantitative analysis of the microalgal diatom chaetoceros calcitrans |
| url | http://psasir.upm.edu.my/id/eprint/86651/1/Mass%20spectrometry-based%20.pdf |
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