Mechanism of action of cocoa on bone metabolism in calcium- and estrogen- deficient rat model of osteoporosis: evidence for site and dose-related responses and involvement of IGF-I
The present study evaluated the impact of cocoa on skeleton of ovariectomized rats. Animals were randomized into four groups: sham (basal diet: AIN-93M Ca and P-deficient), and three ovariectomized groups: OVX (basal diet), C6 and C12 (6% and 12% cocoa powder, respectively). Urinary calcium loss was...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86703/1/Mechanism%20of%20action%20of%20cocoa%20on%20bone%20metabolism%20in%20calcium-%20and%20estrogen-%20deficient%20rat%20model%20of%20osteoporosis.pdf |
| _version_ | 1796981742833238016 |
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| author | Sarmadi, Bahareh Ismail, Amin Yusof, Loqman Mod Yunoh, Mohd Faridz |
| author_facet | Sarmadi, Bahareh Ismail, Amin Yusof, Loqman Mod Yunoh, Mohd Faridz |
| author_sort | Sarmadi, Bahareh |
| collection | UPM |
| description | The present study evaluated the impact of cocoa on skeleton of ovariectomized rats. Animals were randomized into four groups: sham (basal diet: AIN-93M Ca and P-deficient), and three ovariectomized groups: OVX (basal diet), C6 and C12 (6% and 12% cocoa powder, respectively). Urinary calcium loss was decreased in C6 but not C12, while serum insulin-like growth factor (IGF)-I increased in both cocoa groups. Improvement in bone quality and strength was observed in femur mid-shaft in C6 but not C12. Bone density, quality and strength of lumbar vertebrae were improved in both cocoa groups. Cocoa improved systemic oxidative stress and inflammatory biomarkers. In bone tissue of C12, higher levels of H2O2 and lower levels of glutathione peroxidase (GPx) were observed. Cocoa down-regulated osteoblast- and osteoclast-related genes while up-regulated GPx and superoxide dismutase (SOD). Cocoa decreased bone turnover in ovariectomized rats. Its effects depended on the dose applied and the bone site. |
| first_indexed | 2024-03-06T10:42:06Z |
| format | Article |
| id | upm.eprints-86703 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:42:06Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-867032021-11-09T07:34:36Z http://psasir.upm.edu.my/id/eprint/86703/ Mechanism of action of cocoa on bone metabolism in calcium- and estrogen- deficient rat model of osteoporosis: evidence for site and dose-related responses and involvement of IGF-I Sarmadi, Bahareh Ismail, Amin Yusof, Loqman Mod Yunoh, Mohd Faridz The present study evaluated the impact of cocoa on skeleton of ovariectomized rats. Animals were randomized into four groups: sham (basal diet: AIN-93M Ca and P-deficient), and three ovariectomized groups: OVX (basal diet), C6 and C12 (6% and 12% cocoa powder, respectively). Urinary calcium loss was decreased in C6 but not C12, while serum insulin-like growth factor (IGF)-I increased in both cocoa groups. Improvement in bone quality and strength was observed in femur mid-shaft in C6 but not C12. Bone density, quality and strength of lumbar vertebrae were improved in both cocoa groups. Cocoa improved systemic oxidative stress and inflammatory biomarkers. In bone tissue of C12, higher levels of H2O2 and lower levels of glutathione peroxidase (GPx) were observed. Cocoa down-regulated osteoblast- and osteoclast-related genes while up-regulated GPx and superoxide dismutase (SOD). Cocoa decreased bone turnover in ovariectomized rats. Its effects depended on the dose applied and the bone site. Elsevier 2020-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86703/1/Mechanism%20of%20action%20of%20cocoa%20on%20bone%20metabolism%20in%20calcium-%20and%20estrogen-%20deficient%20rat%20model%20of%20osteoporosis.pdf Sarmadi, Bahareh and Ismail, Amin and Yusof, Loqman and Mod Yunoh, Mohd Faridz (2020) Mechanism of action of cocoa on bone metabolism in calcium- and estrogen- deficient rat model of osteoporosis: evidence for site and dose-related responses and involvement of IGF-I. Journal of Functional Foods, 66. art. no. 103793. pp. 1-12. ISSN 1756-4646; ESSN: 2214-9414 https://www.sciencedirect.com/science/article/pii/S1756464620300177 10.1016/j.jff.2020.103793 |
| spellingShingle | Sarmadi, Bahareh Ismail, Amin Yusof, Loqman Mod Yunoh, Mohd Faridz Mechanism of action of cocoa on bone metabolism in calcium- and estrogen- deficient rat model of osteoporosis: evidence for site and dose-related responses and involvement of IGF-I |
| title | Mechanism of action of cocoa on bone metabolism in calcium- and estrogen- deficient rat model of osteoporosis: evidence for site and dose-related responses and involvement of IGF-I |
| title_full | Mechanism of action of cocoa on bone metabolism in calcium- and estrogen- deficient rat model of osteoporosis: evidence for site and dose-related responses and involvement of IGF-I |
| title_fullStr | Mechanism of action of cocoa on bone metabolism in calcium- and estrogen- deficient rat model of osteoporosis: evidence for site and dose-related responses and involvement of IGF-I |
| title_full_unstemmed | Mechanism of action of cocoa on bone metabolism in calcium- and estrogen- deficient rat model of osteoporosis: evidence for site and dose-related responses and involvement of IGF-I |
| title_short | Mechanism of action of cocoa on bone metabolism in calcium- and estrogen- deficient rat model of osteoporosis: evidence for site and dose-related responses and involvement of IGF-I |
| title_sort | mechanism of action of cocoa on bone metabolism in calcium and estrogen deficient rat model of osteoporosis evidence for site and dose related responses and involvement of igf i |
| url | http://psasir.upm.edu.my/id/eprint/86703/1/Mechanism%20of%20action%20of%20cocoa%20on%20bone%20metabolism%20in%20calcium-%20and%20estrogen-%20deficient%20rat%20model%20of%20osteoporosis.pdf |
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