Metabolites identification of oil palm roots infected with Ganoderma boninense using GC-MS-based metabolomics
An approach to metabolomics profiling of non-infected and Ganoderma boninense (G. boninsense) infected oil palm roots crude extracts that utilize gas chromatography-mass spectrometry (GC–MS) and multivariate statistics of principal component analysis (PCA) have been tested. This combination has prov...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86730/1/Metabolites%20identification%20of%20oil%20palm%20roots%20infected%20.pdf |
| _version_ | 1796981747812925440 |
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| author | Isha, Azizul Yusof, Nor Azah Shaari, Khozirah Osman, Rosiah Abdullah, Siti Nor Akmar Wong, Mui Yun |
| author_facet | Isha, Azizul Yusof, Nor Azah Shaari, Khozirah Osman, Rosiah Abdullah, Siti Nor Akmar Wong, Mui Yun |
| author_sort | Isha, Azizul |
| collection | UPM |
| description | An approach to metabolomics profiling of non-infected and Ganoderma boninense (G. boninsense) infected oil palm roots crude extracts that utilize gas chromatography-mass spectrometry (GC–MS) and multivariate statistics of principal component analysis (PCA) have been tested. This combination has provided a rapid approach in investigating the changes in the metabolite variations of non-infected and infected oil palm roots at 14 and 30 days post-infection. The extracts were prepared by using 80% (v/v) of methanol. In identifying the metabolites responsible for each differentiation, PCA model was generated in loading bi-plot. Dimethyl benzene-1,4-dicarboxylate, methyl 3-(3,5-ditert-butyl-4-hydroxyphenyl)propanoate, ergost-5-en-3-ol, (3β), stigmast-5-en-3-ol, (3β), stigmasterol, methyl hexadecanoate, methyl (9Z,12Z)-octadeca-9,12-dienoate, methyl octadecanoate, 2-(hydroxymethyl)-2-nitropropane-1,3-diol, methyl (Z)-octadec-6-enoate and (E)-icos-5-ene were found more abundant in G. boninense infected roots than in non-infected roots. Steroidal compounds and fatty acid derivatives which has been determined in the non-infected and G. boninense infected roots regulate a variety of responses to the G. boninense. The abundant of these metabolites in G. boninense infected roots are due to the crucial roles in pathogen defence. |
| first_indexed | 2024-03-06T10:42:10Z |
| format | Article |
| id | upm.eprints-86730 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:42:10Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-867302021-11-09T08:58:43Z http://psasir.upm.edu.my/id/eprint/86730/ Metabolites identification of oil palm roots infected with Ganoderma boninense using GC-MS-based metabolomics Isha, Azizul Yusof, Nor Azah Shaari, Khozirah Osman, Rosiah Abdullah, Siti Nor Akmar Wong, Mui Yun An approach to metabolomics profiling of non-infected and Ganoderma boninense (G. boninsense) infected oil palm roots crude extracts that utilize gas chromatography-mass spectrometry (GC–MS) and multivariate statistics of principal component analysis (PCA) have been tested. This combination has provided a rapid approach in investigating the changes in the metabolite variations of non-infected and infected oil palm roots at 14 and 30 days post-infection. The extracts were prepared by using 80% (v/v) of methanol. In identifying the metabolites responsible for each differentiation, PCA model was generated in loading bi-plot. Dimethyl benzene-1,4-dicarboxylate, methyl 3-(3,5-ditert-butyl-4-hydroxyphenyl)propanoate, ergost-5-en-3-ol, (3β), stigmast-5-en-3-ol, (3β), stigmasterol, methyl hexadecanoate, methyl (9Z,12Z)-octadeca-9,12-dienoate, methyl octadecanoate, 2-(hydroxymethyl)-2-nitropropane-1,3-diol, methyl (Z)-octadec-6-enoate and (E)-icos-5-ene were found more abundant in G. boninense infected roots than in non-infected roots. Steroidal compounds and fatty acid derivatives which has been determined in the non-infected and G. boninense infected roots regulate a variety of responses to the G. boninense. The abundant of these metabolites in G. boninense infected roots are due to the crucial roles in pathogen defence. Elsevier 2020-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86730/1/Metabolites%20identification%20of%20oil%20palm%20roots%20infected%20.pdf Isha, Azizul and Yusof, Nor Azah and Shaari, Khozirah and Osman, Rosiah and Abdullah, Siti Nor Akmar and Wong, Mui Yun (2020) Metabolites identification of oil palm roots infected with Ganoderma boninense using GC-MS-based metabolomics. Arabian Journal of Chemistry, 13 (7). 6191 - 6200. ISSN 1878-5352; ESSN: 1878-5379 https://www.sciencedirect.com/science/article/pii/S1878535220301805 10.1016/j.arabjc.2020.05.026 |
| spellingShingle | Isha, Azizul Yusof, Nor Azah Shaari, Khozirah Osman, Rosiah Abdullah, Siti Nor Akmar Wong, Mui Yun Metabolites identification of oil palm roots infected with Ganoderma boninense using GC-MS-based metabolomics |
| title | Metabolites identification of oil palm roots infected with Ganoderma boninense using GC-MS-based metabolomics |
| title_full | Metabolites identification of oil palm roots infected with Ganoderma boninense using GC-MS-based metabolomics |
| title_fullStr | Metabolites identification of oil palm roots infected with Ganoderma boninense using GC-MS-based metabolomics |
| title_full_unstemmed | Metabolites identification of oil palm roots infected with Ganoderma boninense using GC-MS-based metabolomics |
| title_short | Metabolites identification of oil palm roots infected with Ganoderma boninense using GC-MS-based metabolomics |
| title_sort | metabolites identification of oil palm roots infected with ganoderma boninense using gc ms based metabolomics |
| url | http://psasir.upm.edu.my/id/eprint/86730/1/Metabolites%20identification%20of%20oil%20palm%20roots%20infected%20.pdf |
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