Molecular characterization and phylogenetic analysis of Pantoea stewartii subspecies stewartii causing bronzing disease of jackfruit in Malaysia based on cps and hrp gene sequences
Bronzing disease of jackfruit was first detected in plantation areas in Selangor and Pahang, Malaysia. The symptoms included reddish discoloration of the affected fruit pulp and rags, which could reduce the fruit quality and discourage purchasers. In this study, species-specific PCR amplification wi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86809/1/Molecular%20characterization%20and%20phylogenetic%20analysis.pdf |
| _version_ | 1825952267129323520 |
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| author | Ibrahim, Rohaya Ismail Suhaimy, Noor Wahida Tan, Shu Qing Ismail, Siti Izera Abidin, Nuraizat Hakiman, Mansor Karam, Daljit Singh Ahmad Hamdani, Muhammad Saiful Yusof, Mohd Termizi Zulperi, Dzarifah |
| author_facet | Ibrahim, Rohaya Ismail Suhaimy, Noor Wahida Tan, Shu Qing Ismail, Siti Izera Abidin, Nuraizat Hakiman, Mansor Karam, Daljit Singh Ahmad Hamdani, Muhammad Saiful Yusof, Mohd Termizi Zulperi, Dzarifah |
| author_sort | Ibrahim, Rohaya |
| collection | UPM |
| description | Bronzing disease of jackfruit was first detected in plantation areas in Selangor and Pahang, Malaysia. The symptoms included reddish discoloration of the affected fruit pulp and rags, which could reduce the fruit quality and discourage purchasers. In this study, species-specific PCR amplification with primers encoded for cps and hrp virulence genes showed all bacterial strains isolated from infected jackfruits with bronzing symptoms, displaying a 1.1 kb and 0.9 kb amplicon. Phylogenetic analyses of the cps and hrp gene sequences clustered all strains to Pantoea stewartii subspecies stewartii (P. stewartii subsp. stewartii) reference strains (GenBank accession Nos. AF077292, AF282857, KY965964 and KY965965), with 62 to 80% similarity. Results revealed the presence of these virulence genes in P. stewartii subsp. stewartii strains is required for pathogenicity of the bacterium in jackfruit. |
| first_indexed | 2024-03-06T10:42:22Z |
| format | Article |
| id | upm.eprints-86809 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:42:22Z |
| publishDate | 2020 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-868092021-11-17T11:06:19Z http://psasir.upm.edu.my/id/eprint/86809/ Molecular characterization and phylogenetic analysis of Pantoea stewartii subspecies stewartii causing bronzing disease of jackfruit in Malaysia based on cps and hrp gene sequences Ibrahim, Rohaya Ismail Suhaimy, Noor Wahida Tan, Shu Qing Ismail, Siti Izera Abidin, Nuraizat Hakiman, Mansor Karam, Daljit Singh Ahmad Hamdani, Muhammad Saiful Yusof, Mohd Termizi Zulperi, Dzarifah Bronzing disease of jackfruit was first detected in plantation areas in Selangor and Pahang, Malaysia. The symptoms included reddish discoloration of the affected fruit pulp and rags, which could reduce the fruit quality and discourage purchasers. In this study, species-specific PCR amplification with primers encoded for cps and hrp virulence genes showed all bacterial strains isolated from infected jackfruits with bronzing symptoms, displaying a 1.1 kb and 0.9 kb amplicon. Phylogenetic analyses of the cps and hrp gene sequences clustered all strains to Pantoea stewartii subspecies stewartii (P. stewartii subsp. stewartii) reference strains (GenBank accession Nos. AF077292, AF282857, KY965964 and KY965965), with 62 to 80% similarity. Results revealed the presence of these virulence genes in P. stewartii subsp. stewartii strains is required for pathogenicity of the bacterium in jackfruit. Springer 2020-08-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86809/1/Molecular%20characterization%20and%20phylogenetic%20analysis.pdf Ibrahim, Rohaya and Ismail Suhaimy, Noor Wahida and Tan, Shu Qing and Ismail, Siti Izera and Abidin, Nuraizat and Hakiman, Mansor and Karam, Daljit Singh and Ahmad Hamdani, Muhammad Saiful and Yusof, Mohd Termizi and Zulperi, Dzarifah (2020) Molecular characterization and phylogenetic analysis of Pantoea stewartii subspecies stewartii causing bronzing disease of jackfruit in Malaysia based on cps and hrp gene sequences. Journal of Plant Pathology, 102. 193 - 199. ISSN 1125-4653; ESSN: 2239-7264 https://link.springer.com/article/10.1007/s42161-019-00383-7 10.1007/s42161-019-00383-7 |
| spellingShingle | Ibrahim, Rohaya Ismail Suhaimy, Noor Wahida Tan, Shu Qing Ismail, Siti Izera Abidin, Nuraizat Hakiman, Mansor Karam, Daljit Singh Ahmad Hamdani, Muhammad Saiful Yusof, Mohd Termizi Zulperi, Dzarifah Molecular characterization and phylogenetic analysis of Pantoea stewartii subspecies stewartii causing bronzing disease of jackfruit in Malaysia based on cps and hrp gene sequences |
| title | Molecular characterization and phylogenetic analysis of Pantoea stewartii subspecies stewartii causing bronzing disease of jackfruit in Malaysia based on cps and hrp gene sequences |
| title_full | Molecular characterization and phylogenetic analysis of Pantoea stewartii subspecies stewartii causing bronzing disease of jackfruit in Malaysia based on cps and hrp gene sequences |
| title_fullStr | Molecular characterization and phylogenetic analysis of Pantoea stewartii subspecies stewartii causing bronzing disease of jackfruit in Malaysia based on cps and hrp gene sequences |
| title_full_unstemmed | Molecular characterization and phylogenetic analysis of Pantoea stewartii subspecies stewartii causing bronzing disease of jackfruit in Malaysia based on cps and hrp gene sequences |
| title_short | Molecular characterization and phylogenetic analysis of Pantoea stewartii subspecies stewartii causing bronzing disease of jackfruit in Malaysia based on cps and hrp gene sequences |
| title_sort | molecular characterization and phylogenetic analysis of pantoea stewartii subspecies stewartii causing bronzing disease of jackfruit in malaysia based on cps and hrp gene sequences |
| url | http://psasir.upm.edu.my/id/eprint/86809/1/Molecular%20characterization%20and%20phylogenetic%20analysis.pdf |
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