Molecular confirmation of candidate Hsp70 gene associated with heat tolerance in BC3F2 advanced backcross lines and their phenotypic resemblance with recurrent chilli Kulai
Domestic production of chilli in Malaysia is insufficient which accounted for 52% self-sufficiency level. Poor performance of local varieties under high temperatures above 42°C is one of the major factors affecting chilli production. This study was conducted with the objective of introgress heat-tol...
| Main Authors: | , , , , , |
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| פורמט: | Article |
| שפה: | English |
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Taylor & Francis
2020
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| גישה מקוונת: | http://psasir.upm.edu.my/id/eprint/86811/1/Molecular%20confirmation%20of%20candidate%20Hsp70.pdf |
| _version_ | 1825952267545608192 |
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| author | Usman, Magaji G. Rafii, Mohd Yusop Yusuff, Oladosu Mohammad Yusoff, Martini Ismail, Mohd R. Ridzuan, Raihana |
| author_facet | Usman, Magaji G. Rafii, Mohd Yusop Yusuff, Oladosu Mohammad Yusoff, Martini Ismail, Mohd R. Ridzuan, Raihana |
| author_sort | Usman, Magaji G. |
| collection | UPM |
| description | Domestic production of chilli in Malaysia is insufficient which accounted for 52% self-sufficiency level. Poor performance of local varieties under high temperatures above 42°C is one of the major factors affecting chilli production. This study was conducted with the objective of introgress heat-tolerant gene (Hsps) from AVPP0702 donor into Kulai using marker-assisted backcrossing. A total of 68 SSR markers, including Hsp linked markers that showed polymorphism between the parents were used to assess the generation of backcrossing; BC1F1, BC2F1, BC3F1 and BC3F2 and the average RPG percentage of the recurrent parent was found to be 81, 90, 95 and 97%, respectively. The pattern of Hsp expression in the backcross generations was similar to the donor parent (upregulated with more than 4-fold increase). Twelve improved heat tolerant chilli lines were developed. Most of the morphological and agronomical traits were recovered in the selected improved heat-tolerant genotypes from Kulai such as plant height, number of days to 50% flowering, number of fruits, fruit length and total fruit yield per plant. Improved high-yielding heat-tolerant chilli lines showed tolerance to high temperature as well as did not express any negative effect on agronomic traits in comparison with Kulai variety. |
| first_indexed | 2024-03-06T10:42:22Z |
| format | Article |
| id | upm.eprints-86811 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:42:22Z |
| publishDate | 2020 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | upm.eprints-868112021-11-18T07:03:21Z http://psasir.upm.edu.my/id/eprint/86811/ Molecular confirmation of candidate Hsp70 gene associated with heat tolerance in BC3F2 advanced backcross lines and their phenotypic resemblance with recurrent chilli Kulai Usman, Magaji G. Rafii, Mohd Yusop Yusuff, Oladosu Mohammad Yusoff, Martini Ismail, Mohd R. Ridzuan, Raihana Domestic production of chilli in Malaysia is insufficient which accounted for 52% self-sufficiency level. Poor performance of local varieties under high temperatures above 42°C is one of the major factors affecting chilli production. This study was conducted with the objective of introgress heat-tolerant gene (Hsps) from AVPP0702 donor into Kulai using marker-assisted backcrossing. A total of 68 SSR markers, including Hsp linked markers that showed polymorphism between the parents were used to assess the generation of backcrossing; BC1F1, BC2F1, BC3F1 and BC3F2 and the average RPG percentage of the recurrent parent was found to be 81, 90, 95 and 97%, respectively. The pattern of Hsp expression in the backcross generations was similar to the donor parent (upregulated with more than 4-fold increase). Twelve improved heat tolerant chilli lines were developed. Most of the morphological and agronomical traits were recovered in the selected improved heat-tolerant genotypes from Kulai such as plant height, number of days to 50% flowering, number of fruits, fruit length and total fruit yield per plant. Improved high-yielding heat-tolerant chilli lines showed tolerance to high temperature as well as did not express any negative effect on agronomic traits in comparison with Kulai variety. Taylor & Francis 2020-11-26 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86811/1/Molecular%20confirmation%20of%20candidate%20Hsp70.pdf Usman, Magaji G. and Rafii, Mohd Yusop and Yusuff, Oladosu and Mohammad Yusoff, Martini and Ismail, Mohd R. and Ridzuan, Raihana (2020) Molecular confirmation of candidate Hsp70 gene associated with heat tolerance in BC3F2 advanced backcross lines and their phenotypic resemblance with recurrent chilli Kulai. Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, 70 (3). 252 - 264. ISSN 0906-4710; ESSN: 1651-1913 https://www.tandfonline.com/doi/abs/10.1080/09064710.2019.1692899 10.1080/09064710.2019.1692899 |
| spellingShingle | Usman, Magaji G. Rafii, Mohd Yusop Yusuff, Oladosu Mohammad Yusoff, Martini Ismail, Mohd R. Ridzuan, Raihana Molecular confirmation of candidate Hsp70 gene associated with heat tolerance in BC3F2 advanced backcross lines and their phenotypic resemblance with recurrent chilli Kulai |
| title | Molecular confirmation of candidate Hsp70 gene associated with heat tolerance in BC3F2 advanced backcross lines and their phenotypic resemblance with recurrent chilli Kulai |
| title_full | Molecular confirmation of candidate Hsp70 gene associated with heat tolerance in BC3F2 advanced backcross lines and their phenotypic resemblance with recurrent chilli Kulai |
| title_fullStr | Molecular confirmation of candidate Hsp70 gene associated with heat tolerance in BC3F2 advanced backcross lines and their phenotypic resemblance with recurrent chilli Kulai |
| title_full_unstemmed | Molecular confirmation of candidate Hsp70 gene associated with heat tolerance in BC3F2 advanced backcross lines and their phenotypic resemblance with recurrent chilli Kulai |
| title_short | Molecular confirmation of candidate Hsp70 gene associated with heat tolerance in BC3F2 advanced backcross lines and their phenotypic resemblance with recurrent chilli Kulai |
| title_sort | molecular confirmation of candidate hsp70 gene associated with heat tolerance in bc3f2 advanced backcross lines and their phenotypic resemblance with recurrent chilli kulai |
| url | http://psasir.upm.edu.my/id/eprint/86811/1/Molecular%20confirmation%20of%20candidate%20Hsp70.pdf |
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