Molecular sex identification of Malaysian white-nest swiftlet (Aerodramus fuciphagus Thunberg, 1812)
The difficulty in differentiating the sex of monomorphic bird species has made molecular sexing an important tool in addressing this problem. This method uses noninvasively collected materials such as feathers and may be advantageous for sexing endangered as well as commercialized bird species. In t...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley Open Access
2020
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/86822/1/Molecular%20sex%20identification%20of%20Malaysian%20white.pdf |
| _version_ | 1796981763474456576 |
|---|---|
| author | Osman, Muhammad Amin Sugnaseelan, Sumita Panandam, Jothi Malar Ab Ghani, Nurul Izza |
| author_facet | Osman, Muhammad Amin Sugnaseelan, Sumita Panandam, Jothi Malar Ab Ghani, Nurul Izza |
| author_sort | Osman, Muhammad Amin |
| collection | UPM |
| description | The difficulty in differentiating the sex of monomorphic bird species has made molecular sexing an important tool in addressing this problem. This method uses noninvasively collected materials such as feathers and may be advantageous for sexing endangered as well as commercialized bird species. In this study, seven primer sets for sexing birds were screened in Aerodramus fuciphagus using a total of 13 feather samples that were randomly selected from the state of Perak, Malaysia. From the screening analysis, only one primer set (P8/WZ/W) successfully differentiated the sex of A. fuciphagus. PCR amplification produced a single 255-bp DNA fragment for males which was derived from CHD-Z (CHD gene region in the sex chromosome Z), while for the females it produced two fragments (144 and 255 bp). The 144-bp fragment was from CHD-W (CHD gene region in the sex chromosome W). Results from sequencing showed no variations in the base sequences of the CHD-W and CHD-Z amplified fragments within the same sexes, except for one male sample (A23) where at position 166, a base substitution occurred (G → A). Phylogenetic analysis of CHD-W showed that four (Apodiformes; Gruiformes; Passeriformes; and Pelecaniformes) out of the five orders investigated had formed four clear clusters within their orders, including the studied order: Apodiformes. Whereas in CHD-Z, four (Accipitriformes; Columbiformes; Galliformes; and Passeriformes) out of five orders investigated formed four clear clusters within their orders, excluding the studied order. In addition, A. fuciphagus and Apus apus (both Apodiformes) showed less divergence in CHD-W than CHD-Z (0% c.f. 9%). The result suggests that in A. fuciphagus, CHD gene evolution occurred at a higher rate in males (CHD-Z) compared to females (CHD-W). This finding may be useful for further studies on sex ratio and breeding management of A. fuciphagus. |
| first_indexed | 2024-03-06T10:42:24Z |
| format | Article |
| id | upm.eprints-86822 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:42:24Z |
| publishDate | 2020 |
| publisher | Wiley Open Access |
| record_format | dspace |
| spelling | upm.eprints-868222021-11-17T10:45:37Z http://psasir.upm.edu.my/id/eprint/86822/ Molecular sex identification of Malaysian white-nest swiftlet (Aerodramus fuciphagus Thunberg, 1812) Osman, Muhammad Amin Sugnaseelan, Sumita Panandam, Jothi Malar Ab Ghani, Nurul Izza The difficulty in differentiating the sex of monomorphic bird species has made molecular sexing an important tool in addressing this problem. This method uses noninvasively collected materials such as feathers and may be advantageous for sexing endangered as well as commercialized bird species. In this study, seven primer sets for sexing birds were screened in Aerodramus fuciphagus using a total of 13 feather samples that were randomly selected from the state of Perak, Malaysia. From the screening analysis, only one primer set (P8/WZ/W) successfully differentiated the sex of A. fuciphagus. PCR amplification produced a single 255-bp DNA fragment for males which was derived from CHD-Z (CHD gene region in the sex chromosome Z), while for the females it produced two fragments (144 and 255 bp). The 144-bp fragment was from CHD-W (CHD gene region in the sex chromosome W). Results from sequencing showed no variations in the base sequences of the CHD-W and CHD-Z amplified fragments within the same sexes, except for one male sample (A23) where at position 166, a base substitution occurred (G → A). Phylogenetic analysis of CHD-W showed that four (Apodiformes; Gruiformes; Passeriformes; and Pelecaniformes) out of the five orders investigated had formed four clear clusters within their orders, including the studied order: Apodiformes. Whereas in CHD-Z, four (Accipitriformes; Columbiformes; Galliformes; and Passeriformes) out of five orders investigated formed four clear clusters within their orders, excluding the studied order. In addition, A. fuciphagus and Apus apus (both Apodiformes) showed less divergence in CHD-W than CHD-Z (0% c.f. 9%). The result suggests that in A. fuciphagus, CHD gene evolution occurred at a higher rate in males (CHD-Z) compared to females (CHD-W). This finding may be useful for further studies on sex ratio and breeding management of A. fuciphagus. Wiley Open Access 2020-10 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86822/1/Molecular%20sex%20identification%20of%20Malaysian%20white.pdf Osman, Muhammad Amin and Sugnaseelan, Sumita and Panandam, Jothi Malar and Ab Ghani, Nurul Izza (2020) Molecular sex identification of Malaysian white-nest swiftlet (Aerodramus fuciphagus Thunberg, 1812). Ecology and Evolution, 10 (19). 10440 - 10448. ISSN 2045-7758 https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.6699 10.1002/ece3.6699 |
| spellingShingle | Osman, Muhammad Amin Sugnaseelan, Sumita Panandam, Jothi Malar Ab Ghani, Nurul Izza Molecular sex identification of Malaysian white-nest swiftlet (Aerodramus fuciphagus Thunberg, 1812) |
| title | Molecular sex identification of Malaysian white-nest swiftlet (Aerodramus fuciphagus Thunberg, 1812) |
| title_full | Molecular sex identification of Malaysian white-nest swiftlet (Aerodramus fuciphagus Thunberg, 1812) |
| title_fullStr | Molecular sex identification of Malaysian white-nest swiftlet (Aerodramus fuciphagus Thunberg, 1812) |
| title_full_unstemmed | Molecular sex identification of Malaysian white-nest swiftlet (Aerodramus fuciphagus Thunberg, 1812) |
| title_short | Molecular sex identification of Malaysian white-nest swiftlet (Aerodramus fuciphagus Thunberg, 1812) |
| title_sort | molecular sex identification of malaysian white nest swiftlet aerodramus fuciphagus thunberg 1812 |
| url | http://psasir.upm.edu.my/id/eprint/86822/1/Molecular%20sex%20identification%20of%20Malaysian%20white.pdf |
| work_keys_str_mv | AT osmanmuhammadamin molecularsexidentificationofmalaysianwhitenestswiftletaerodramusfuciphagusthunberg1812 AT sugnaseelansumita molecularsexidentificationofmalaysianwhitenestswiftletaerodramusfuciphagusthunberg1812 AT panandamjothimalar molecularsexidentificationofmalaysianwhitenestswiftletaerodramusfuciphagusthunberg1812 AT abghaninurulizza molecularsexidentificationofmalaysianwhitenestswiftletaerodramusfuciphagusthunberg1812 |