Morphological, phylogenetic and pathogenicity characterisation of Fusarium species associated with wilt disease of pumpkin (Cucurbita pepo Linnaeus)
Fusarium is a well-known soil-borne fungus where most species belonged in this genus is prominently phytopathogenic. Nevertheless, this pathogenic species has affected the production of pumpkin worldwide. This study underlines the morphological, phylogeny and pathogenicity characteristics of Fusar...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Life Sciences Society
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86833/1/Morphological%20phylogenetic%20and%20pathogenicity.pdf |
| _version_ | 1796981765447876608 |
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| author | Aris, Asma Ab Easa Hasan, Zainap Shohaimi, Shamarina Saidi, Noor Baity Mohd Zainudin, Nur Ain Izzati |
| author_facet | Aris, Asma Ab Easa Hasan, Zainap Shohaimi, Shamarina Saidi, Noor Baity Mohd Zainudin, Nur Ain Izzati |
| author_sort | Aris, Asma |
| collection | UPM |
| description | Fusarium is a well-known soil-borne fungus where most species belonged in this genus is prominently phytopathogenic. Nevertheless, this pathogenic species has affected the production of pumpkin worldwide. This study underlines the morphological, phylogeny and pathogenicity characteristics of Fusarium for a better disease-control strategy. Twenty-six Fusarium isolates were collected from wilt infected pumpkin in various locations of Peninsular Malaysia. From the combinations of morphological and molecular identifications, four species were identified as F. oxysporum (2 isolates), F. solani (4 isolates), F. proliferatum (7 isolates) and F. incarnatum (13 isolates). Microscopic and macroscopic observation visualized distinct characteristics of the identified Fusarium species. Sequence analyses of tef1α and β-tub genes inferred by maximum likelihood tree resulted in distinct section-specific characteristics. Meanwhile, pathogenicity test of Fusarium isolates presented by the seed inoculation produced various degrees of severities. Fusarium solani C2526P recorded the highest severity of 93.8% after 30 days of post inoculation (dpi). Symptoms have been identified as early as 10 dpi producing stunted growth of the plants. On the other hand, Fusarium oxysporum D2532P recorded 85.3% disease severity. Pathogenic Fusarium caused stunted growth, chlorosis, wilting and necrosis especially at the root of pumpkin plants. This study provides valuable information and methods to manage wilt infected pumpkin in the future. |
| first_indexed | 2024-03-06T10:42:26Z |
| format | Article |
| id | upm.eprints-86833 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:42:26Z |
| publishDate | 2020 |
| publisher | Life Sciences Society |
| record_format | dspace |
| spelling | upm.eprints-868332021-11-20T11:52:24Z http://psasir.upm.edu.my/id/eprint/86833/ Morphological, phylogenetic and pathogenicity characterisation of Fusarium species associated with wilt disease of pumpkin (Cucurbita pepo Linnaeus) Aris, Asma Ab Easa Hasan, Zainap Shohaimi, Shamarina Saidi, Noor Baity Mohd Zainudin, Nur Ain Izzati Fusarium is a well-known soil-borne fungus where most species belonged in this genus is prominently phytopathogenic. Nevertheless, this pathogenic species has affected the production of pumpkin worldwide. This study underlines the morphological, phylogeny and pathogenicity characteristics of Fusarium for a better disease-control strategy. Twenty-six Fusarium isolates were collected from wilt infected pumpkin in various locations of Peninsular Malaysia. From the combinations of morphological and molecular identifications, four species were identified as F. oxysporum (2 isolates), F. solani (4 isolates), F. proliferatum (7 isolates) and F. incarnatum (13 isolates). Microscopic and macroscopic observation visualized distinct characteristics of the identified Fusarium species. Sequence analyses of tef1α and β-tub genes inferred by maximum likelihood tree resulted in distinct section-specific characteristics. Meanwhile, pathogenicity test of Fusarium isolates presented by the seed inoculation produced various degrees of severities. Fusarium solani C2526P recorded the highest severity of 93.8% after 30 days of post inoculation (dpi). Symptoms have been identified as early as 10 dpi producing stunted growth of the plants. On the other hand, Fusarium oxysporum D2532P recorded 85.3% disease severity. Pathogenic Fusarium caused stunted growth, chlorosis, wilting and necrosis especially at the root of pumpkin plants. This study provides valuable information and methods to manage wilt infected pumpkin in the future. Life Sciences Society 2020-02-17 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86833/1/Morphological%20phylogenetic%20and%20pathogenicity.pdf Aris, Asma and Ab Easa Hasan, Zainap and Shohaimi, Shamarina and Saidi, Noor Baity and Mohd Zainudin, Nur Ain Izzati (2020) Morphological, phylogenetic and pathogenicity characterisation of Fusarium species associated with wilt disease of pumpkin (Cucurbita pepo Linnaeus). Asian Journal of Agriculture and Biology, 8 (1). 75 - 84. ISSN 2307-8553 https://www.asianjab.com/morphological-phylogenetic-and-pathogenicity-characterisation-of-fusarium-species-associated-with-wilt-disease-of-pumpkin-cucurbita-pepo-linnaeus/ 10.35495/ajab.2019.07.319 |
| spellingShingle | Aris, Asma Ab Easa Hasan, Zainap Shohaimi, Shamarina Saidi, Noor Baity Mohd Zainudin, Nur Ain Izzati Morphological, phylogenetic and pathogenicity characterisation of Fusarium species associated with wilt disease of pumpkin (Cucurbita pepo Linnaeus) |
| title | Morphological, phylogenetic and pathogenicity characterisation of Fusarium species associated with wilt disease of pumpkin (Cucurbita pepo Linnaeus) |
| title_full | Morphological, phylogenetic and pathogenicity characterisation of Fusarium species associated with wilt disease of pumpkin (Cucurbita pepo Linnaeus) |
| title_fullStr | Morphological, phylogenetic and pathogenicity characterisation of Fusarium species associated with wilt disease of pumpkin (Cucurbita pepo Linnaeus) |
| title_full_unstemmed | Morphological, phylogenetic and pathogenicity characterisation of Fusarium species associated with wilt disease of pumpkin (Cucurbita pepo Linnaeus) |
| title_short | Morphological, phylogenetic and pathogenicity characterisation of Fusarium species associated with wilt disease of pumpkin (Cucurbita pepo Linnaeus) |
| title_sort | morphological phylogenetic and pathogenicity characterisation of fusarium species associated with wilt disease of pumpkin cucurbita pepo linnaeus |
| url | http://psasir.upm.edu.my/id/eprint/86833/1/Morphological%20phylogenetic%20and%20pathogenicity.pdf |
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