Mucosal responses of brown-marbled grouper Epinephelus fuscoguttatus (Forsskl, 1775) following intraperitoneal infection with Vibrio harveyi
Groupers are popular aquaculture species in South-East Asia, but their cultivation is affected by infectious disease outbreaks. Mucosa-associated lymphoid tissues provide a first-line defence against pathogens; however, few studies are available relating to cellular or proteomic responses of mucosal...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86844/1/Mucosal%20responses%20of%20brown.pdf |
| _version_ | 1825952273921998848 |
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| author | Cheng, Catherine Yun Chieng Mohd Daud, Hassan Md. Yusoff, Fatimah Kim, Dawn Thompson Abdullah, Maha |
| author_facet | Cheng, Catherine Yun Chieng Mohd Daud, Hassan Md. Yusoff, Fatimah Kim, Dawn Thompson Abdullah, Maha |
| author_sort | Cheng, Catherine Yun Chieng |
| collection | UPM |
| description | Groupers are popular aquaculture species in South-East Asia, but their cultivation is affected by infectious disease outbreaks. Mucosa-associated lymphoid tissues provide a first-line defence against pathogens; however, few studies are available relating to cellular or proteomic responses of mucosal immunity in grouper. Skin, gill and intestine were sampled from brown-marbled grouper Epinephelus fuscoguttatus (Forsskål, 1775) at 4 and 96 hr post-infection (hpi) and 7 days post-infection (dpi) following intraperitoneal infection with Vibrio harveyi, and stained with haematoxylin/eosin and Alcian Blue/periodic acid–Schiff. Skin mucus was analysed by 2D-gel electrophoresis, and proteins modulated by the bacterial infection identified. In the infected fish, significant increases in sacciform cells in skin and increased levels of nucleoside diphosphate kinase in mucus were detected at 4 hpi. At 96 hpi, goblet cells containing acidic mucins significantly increased in the intestine, while those containing mixed mucins increased in skin and gills of infected fish. Proteasome subunit alpha type-I and extracellular Cu/Zn superoxide dismutase levels also increased in mucus. Rodlet and mast cells did not appear to respond to the infection. Mucosal tissues of grouper appeared actively involved in response to Vibrio infection. This information may help future research on improving grouper health, production and vaccine development. |
| first_indexed | 2024-03-06T10:42:28Z |
| format | Article |
| id | upm.eprints-86844 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:42:28Z |
| publishDate | 2020 |
| publisher | Wiley |
| record_format | dspace |
| spelling | upm.eprints-868442021-11-22T02:12:45Z http://psasir.upm.edu.my/id/eprint/86844/ Mucosal responses of brown-marbled grouper Epinephelus fuscoguttatus (Forsskl, 1775) following intraperitoneal infection with Vibrio harveyi Cheng, Catherine Yun Chieng Mohd Daud, Hassan Md. Yusoff, Fatimah Kim, Dawn Thompson Abdullah, Maha Groupers are popular aquaculture species in South-East Asia, but their cultivation is affected by infectious disease outbreaks. Mucosa-associated lymphoid tissues provide a first-line defence against pathogens; however, few studies are available relating to cellular or proteomic responses of mucosal immunity in grouper. Skin, gill and intestine were sampled from brown-marbled grouper Epinephelus fuscoguttatus (Forsskål, 1775) at 4 and 96 hr post-infection (hpi) and 7 days post-infection (dpi) following intraperitoneal infection with Vibrio harveyi, and stained with haematoxylin/eosin and Alcian Blue/periodic acid–Schiff. Skin mucus was analysed by 2D-gel electrophoresis, and proteins modulated by the bacterial infection identified. In the infected fish, significant increases in sacciform cells in skin and increased levels of nucleoside diphosphate kinase in mucus were detected at 4 hpi. At 96 hpi, goblet cells containing acidic mucins significantly increased in the intestine, while those containing mixed mucins increased in skin and gills of infected fish. Proteasome subunit alpha type-I and extracellular Cu/Zn superoxide dismutase levels also increased in mucus. Rodlet and mast cells did not appear to respond to the infection. Mucosal tissues of grouper appeared actively involved in response to Vibrio infection. This information may help future research on improving grouper health, production and vaccine development. Wiley 2020-10-23 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86844/1/Mucosal%20responses%20of%20brown.pdf Cheng, Catherine Yun Chieng and Mohd Daud, Hassan and Md. Yusoff, Fatimah and Kim, Dawn Thompson and Abdullah, Maha (2020) Mucosal responses of brown-marbled grouper Epinephelus fuscoguttatus (Forsskl, 1775) following intraperitoneal infection with Vibrio harveyi. Journal of Fish Diseases, 43 (10). 1249 - 1258. ISSN 0140-7775; ESSN: 1365-2761 https://onlinelibrary.wiley.com/doi/abs/10.1111/jfd.13222 10.1111/jfd.13222 |
| spellingShingle | Cheng, Catherine Yun Chieng Mohd Daud, Hassan Md. Yusoff, Fatimah Kim, Dawn Thompson Abdullah, Maha Mucosal responses of brown-marbled grouper Epinephelus fuscoguttatus (Forsskl, 1775) following intraperitoneal infection with Vibrio harveyi |
| title | Mucosal responses of brown-marbled grouper Epinephelus fuscoguttatus (Forsskl, 1775) following intraperitoneal infection with Vibrio harveyi |
| title_full | Mucosal responses of brown-marbled grouper Epinephelus fuscoguttatus (Forsskl, 1775) following intraperitoneal infection with Vibrio harveyi |
| title_fullStr | Mucosal responses of brown-marbled grouper Epinephelus fuscoguttatus (Forsskl, 1775) following intraperitoneal infection with Vibrio harveyi |
| title_full_unstemmed | Mucosal responses of brown-marbled grouper Epinephelus fuscoguttatus (Forsskl, 1775) following intraperitoneal infection with Vibrio harveyi |
| title_short | Mucosal responses of brown-marbled grouper Epinephelus fuscoguttatus (Forsskl, 1775) following intraperitoneal infection with Vibrio harveyi |
| title_sort | mucosal responses of brown marbled grouper epinephelus fuscoguttatus forsskl 1775 following intraperitoneal infection with vibrio harveyi |
| url | http://psasir.upm.edu.my/id/eprint/86844/1/Mucosal%20responses%20of%20brown.pdf |
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