Negligible effect of chicken cytokine IL-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase N1 on host cellular immune responses
In comparison to the extensive characterization of haemagglutinin antibodies of avian influenza virus (AIV), the role of neuraminidase (NA) as an immunogen is less well understood. This study describes the construction and cellular responses of recombinant fowlpox viruses (rFWPV) strain FP9, co-expr...
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| Format: | Article |
| Language: | English |
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Microbiology Society
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86887/1/Negligible%20effect.pdf |
| _version_ | 1825952281415122944 |
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| author | Nor Majid, Nadzreeq Omar, Abdul Rahman Abdul Razak, Mariatulqabtiah |
| author_facet | Nor Majid, Nadzreeq Omar, Abdul Rahman Abdul Razak, Mariatulqabtiah |
| author_sort | Nor Majid, Nadzreeq |
| collection | UPM |
| description | In comparison to the extensive characterization of haemagglutinin antibodies of avian influenza virus (AIV), the role of neuraminidase (NA) as an immunogen is less well understood. This study describes the construction and cellular responses of recombinant fowlpox viruses (rFWPV) strain FP9, co-expressing NA N1 gene of AIV A/Chicken/Malaysia/5858/2004, and chicken IL-12 gene. Our data shows that the N1 and IL-12 proteins were successfully expressed from the recombinants with 48 kD and 70 kD molecular weights, respectively. Upon inoculation into specific-pathogen-free (SPF) chickens at 105 p.f.u. ml−1, levels of CD3+/CD4+ and CD3+/CD8+ populations were higher in the wild-type fowlpox virus FP9 strain, compared to those of rFWPV-N1 and rFWPV-N1-IL-12 at weeks 2 and 5 time points. Furthermore, rFWPV-N1-IL-12 showed a suppressive effect on chicken body weight within 4 weeks after inoculation. We suggest that co-expression of N1 with or without IL-12 offers undesirable quality as a potential AIV vaccine candidate. |
| first_indexed | 2024-03-06T10:42:35Z |
| format | Article |
| id | upm.eprints-86887 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:42:35Z |
| publishDate | 2020 |
| publisher | Microbiology Society |
| record_format | dspace |
| spelling | upm.eprints-868872021-12-30T07:32:25Z http://psasir.upm.edu.my/id/eprint/86887/ Negligible effect of chicken cytokine IL-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase N1 on host cellular immune responses Nor Majid, Nadzreeq Omar, Abdul Rahman Abdul Razak, Mariatulqabtiah In comparison to the extensive characterization of haemagglutinin antibodies of avian influenza virus (AIV), the role of neuraminidase (NA) as an immunogen is less well understood. This study describes the construction and cellular responses of recombinant fowlpox viruses (rFWPV) strain FP9, co-expressing NA N1 gene of AIV A/Chicken/Malaysia/5858/2004, and chicken IL-12 gene. Our data shows that the N1 and IL-12 proteins were successfully expressed from the recombinants with 48 kD and 70 kD molecular weights, respectively. Upon inoculation into specific-pathogen-free (SPF) chickens at 105 p.f.u. ml−1, levels of CD3+/CD4+ and CD3+/CD8+ populations were higher in the wild-type fowlpox virus FP9 strain, compared to those of rFWPV-N1 and rFWPV-N1-IL-12 at weeks 2 and 5 time points. Furthermore, rFWPV-N1-IL-12 showed a suppressive effect on chicken body weight within 4 weeks after inoculation. We suggest that co-expression of N1 with or without IL-12 offers undesirable quality as a potential AIV vaccine candidate. Microbiology Society 2020-05-18 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86887/1/Negligible%20effect.pdf Nor Majid, Nadzreeq and Omar, Abdul Rahman and Abdul Razak, Mariatulqabtiah (2020) Negligible effect of chicken cytokine IL-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase N1 on host cellular immune responses. Journal of General Virology, 101 (7). pp. 1-6. ISSN 0022-1317; ESSN: 1465-2099 https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001428 10.1099/jgv.0.001428 |
| spellingShingle | Nor Majid, Nadzreeq Omar, Abdul Rahman Abdul Razak, Mariatulqabtiah Negligible effect of chicken cytokine IL-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase N1 on host cellular immune responses |
| title | Negligible effect of chicken cytokine IL-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase N1 on host cellular immune responses |
| title_full | Negligible effect of chicken cytokine IL-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase N1 on host cellular immune responses |
| title_fullStr | Negligible effect of chicken cytokine IL-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase N1 on host cellular immune responses |
| title_full_unstemmed | Negligible effect of chicken cytokine IL-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase N1 on host cellular immune responses |
| title_short | Negligible effect of chicken cytokine IL-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase N1 on host cellular immune responses |
| title_sort | negligible effect of chicken cytokine il 12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase n1 on host cellular immune responses |
| url | http://psasir.upm.edu.my/id/eprint/86887/1/Negligible%20effect.pdf |
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