Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica
Background: The potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms an...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86999/1/Optimisation%20of%20biodegradation.pdf |
| _version_ | 1825952300931219456 |
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| author | Ibrahim, Salihu Mohd Zahri, Khadijah Nabilah Peter, Convey Abdul Khalil, Khalilah Claudio, Gomez Fuentes Zulkharnain, Azham Alias, Siti Aisyah Gerardo, González Rocha Ahmad, Siti Aqlima |
| author_facet | Ibrahim, Salihu Mohd Zahri, Khadijah Nabilah Peter, Convey Abdul Khalil, Khalilah Claudio, Gomez Fuentes Zulkharnain, Azham Alias, Siti Aisyah Gerardo, González Rocha Ahmad, Siti Aqlima |
| author_sort | Ibrahim, Salihu |
| collection | UPM |
| description | Background: The potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms and communities. The removal of oils such as canola oil from the environment and wastewater using biological approaches is desirable as the thermal process of oil degradation is expensive and ineffective. Results: Rhodococcus sp. AQ5-07 was found to have high canola oil-degrading ability. Physico-cultural conditions influencing its activity were studied using one-factor-at-a-time (OFAT) and statistical optimisation approaches. Considerable degradation (78.60%) of 3% oil was achieved by this bacterium when incubated with 1.0 g/L ammonium sulphate, 0.3 g/L yeast extract, pH 7.5 and 10% inoculum at 10°C over a 72-h incubation period. Optimisation of the medium conditions using response surface methodology (RSM) resulted in a 9.01% increase in oil degradation (87.61%) when supplemented with 3.5% canola oil, 1.05 g/L ammonium sulphate, 0.28g/L yeast extract, pH 7.5 and 10% inoculum at 12.5°C over the same incubation period. The bacterium was able to tolerate an oil concentration of up to 4.0%, after which decreased bacterial growth and oil degradation were observed. Conclusions: These features make this strain worthy of examination for practical bioremediation of lipid-rich contaminated sites. This is the first report of any waste catering oil degradation by bacteria originating from Antarctica. |
| first_indexed | 2024-03-06T10:42:52Z |
| format | Article |
| id | upm.eprints-86999 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:42:52Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-869992022-01-10T07:50:50Z http://psasir.upm.edu.my/id/eprint/86999/ Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica Ibrahim, Salihu Mohd Zahri, Khadijah Nabilah Peter, Convey Abdul Khalil, Khalilah Claudio, Gomez Fuentes Zulkharnain, Azham Alias, Siti Aisyah Gerardo, González Rocha Ahmad, Siti Aqlima Background: The potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms and communities. The removal of oils such as canola oil from the environment and wastewater using biological approaches is desirable as the thermal process of oil degradation is expensive and ineffective. Results: Rhodococcus sp. AQ5-07 was found to have high canola oil-degrading ability. Physico-cultural conditions influencing its activity were studied using one-factor-at-a-time (OFAT) and statistical optimisation approaches. Considerable degradation (78.60%) of 3% oil was achieved by this bacterium when incubated with 1.0 g/L ammonium sulphate, 0.3 g/L yeast extract, pH 7.5 and 10% inoculum at 10°C over a 72-h incubation period. Optimisation of the medium conditions using response surface methodology (RSM) resulted in a 9.01% increase in oil degradation (87.61%) when supplemented with 3.5% canola oil, 1.05 g/L ammonium sulphate, 0.28g/L yeast extract, pH 7.5 and 10% inoculum at 12.5°C over the same incubation period. The bacterium was able to tolerate an oil concentration of up to 4.0%, after which decreased bacterial growth and oil degradation were observed. Conclusions: These features make this strain worthy of examination for practical bioremediation of lipid-rich contaminated sites. This is the first report of any waste catering oil degradation by bacteria originating from Antarctica. Elsevier 2020-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86999/1/Optimisation%20of%20biodegradation.pdf Ibrahim, Salihu and Mohd Zahri, Khadijah Nabilah and Peter, Convey and Abdul Khalil, Khalilah and Claudio, Gomez Fuentes and Zulkharnain, Azham and Alias, Siti Aisyah and Gerardo, González Rocha and Ahmad, Siti Aqlima (2020) Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica. Electronic Journal of Biotechnology, 48. pp. 1-12. ISSN 0717-3458 https://www.sciencedirect.com/science/article/pii/S0717345820300361 10.1016/j.ejbt.2020.07.005 |
| spellingShingle | Ibrahim, Salihu Mohd Zahri, Khadijah Nabilah Peter, Convey Abdul Khalil, Khalilah Claudio, Gomez Fuentes Zulkharnain, Azham Alias, Siti Aisyah Gerardo, González Rocha Ahmad, Siti Aqlima Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
| title | Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
| title_full | Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
| title_fullStr | Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
| title_full_unstemmed | Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
| title_short | Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
| title_sort | optimisation of biodegradation conditions for waste canola oil by cold adapted rhodococcus sp aq5 07 from antarctica |
| url | http://psasir.upm.edu.my/id/eprint/86999/1/Optimisation%20of%20biodegradation.pdf |
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