PGP 9.5- immunoreactivity in the ovary at different stages of oestrous cycle in rats
The reproductive process in female mammals is characterized by cyclic alteration in the female tract. During the oestrous cycle, the primordial follicle will be developed into the Graafian follicle before the ovulation and it takes place under the influence of hormonal control. Thi...
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| Format: | Article |
| Sprog: | English |
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Egyptian Society of Biological Sciences
2020
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| Online adgang: | http://psasir.upm.edu.my/id/eprint/87137/1/PGP%209.5-%20immunoreactivity.pdf |
| _version_ | 1825952321501134848 |
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| author | Ganabadi, Shanthi Yaakub, Halimatun Gopalsamy, Banulata Al-Saffar, F. J. |
| author_facet | Ganabadi, Shanthi Yaakub, Halimatun Gopalsamy, Banulata Al-Saffar, F. J. |
| author_sort | Ganabadi, Shanthi |
| collection | UPM |
| description | The reproductive process in female mammals is characterized by cyclic alteration in the female tract. During the oestrous cycle, the primordial follicle will be developed into the Graafian follicle before the ovulation and it takes place under the influence of hormonal control. This work was carried out to study the general pattern of innervation in the ovary at different stages of the oestrous cycle. Twenty-four adult female Sprague Dawley rats were used to perform the goal of the study. These rats were sacrificed after the detection of their cycle stage by vaginal smears and the ovaries were fixed and section using a frozen cryostat. Detection of nerve fibers was done using the immunohistochemistry technique. The results from this study showed that there is innervation in the ovary throughout the oestrous cycle indicated by the presence of PGP 9.5-immunoreactive nerve fibres. The number of nerve fibres found during each stage of the oestrous cycle significantly varies; the nerve fibre count during the oestrus stage was significantly higher (P < 0.05) than the nerve fibre count at proestrus, metestrus, and diestrous stage. In conclusion, immunohistochemistry PGP 9.5 marker was a useful approach and indicator for nerve fibers distribution in organs which can be changed with different physiological conditions. |
| first_indexed | 2024-03-06T10:43:05Z |
| format | Article |
| id | upm.eprints-87137 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:43:05Z |
| publishDate | 2020 |
| publisher | Egyptian Society of Biological Sciences |
| record_format | dspace |
| spelling | upm.eprints-871372022-01-13T07:09:49Z http://psasir.upm.edu.my/id/eprint/87137/ PGP 9.5- immunoreactivity in the ovary at different stages of oestrous cycle in rats Ganabadi, Shanthi Yaakub, Halimatun Gopalsamy, Banulata Al-Saffar, F. J. The reproductive process in female mammals is characterized by cyclic alteration in the female tract. During the oestrous cycle, the primordial follicle will be developed into the Graafian follicle before the ovulation and it takes place under the influence of hormonal control. This work was carried out to study the general pattern of innervation in the ovary at different stages of the oestrous cycle. Twenty-four adult female Sprague Dawley rats were used to perform the goal of the study. These rats were sacrificed after the detection of their cycle stage by vaginal smears and the ovaries were fixed and section using a frozen cryostat. Detection of nerve fibers was done using the immunohistochemistry technique. The results from this study showed that there is innervation in the ovary throughout the oestrous cycle indicated by the presence of PGP 9.5-immunoreactive nerve fibres. The number of nerve fibres found during each stage of the oestrous cycle significantly varies; the nerve fibre count during the oestrus stage was significantly higher (P < 0.05) than the nerve fibre count at proestrus, metestrus, and diestrous stage. In conclusion, immunohistochemistry PGP 9.5 marker was a useful approach and indicator for nerve fibers distribution in organs which can be changed with different physiological conditions. Egyptian Society of Biological Sciences 2020-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87137/1/PGP%209.5-%20immunoreactivity.pdf Ganabadi, Shanthi and Yaakub, Halimatun and Gopalsamy, Banulata and Al-Saffar, F. J. (2020) PGP 9.5- immunoreactivity in the ovary at different stages of oestrous cycle in rats. Egyptian Academic Journal of Biological Sciences, 12 (2). pp. 1-7. ISSN 2090–0775 https://eajbsd.journals.ekb.eg/article_100761.html 10.21608/EAJBSD.2020.100761 |
| spellingShingle | Ganabadi, Shanthi Yaakub, Halimatun Gopalsamy, Banulata Al-Saffar, F. J. PGP 9.5- immunoreactivity in the ovary at different stages of oestrous cycle in rats |
| title | PGP 9.5- immunoreactivity in the ovary at different stages of oestrous cycle in rats |
| title_full | PGP 9.5- immunoreactivity in the ovary at different stages of oestrous cycle in rats |
| title_fullStr | PGP 9.5- immunoreactivity in the ovary at different stages of oestrous cycle in rats |
| title_full_unstemmed | PGP 9.5- immunoreactivity in the ovary at different stages of oestrous cycle in rats |
| title_short | PGP 9.5- immunoreactivity in the ovary at different stages of oestrous cycle in rats |
| title_sort | pgp 9 5 immunoreactivity in the ovary at different stages of oestrous cycle in rats |
| url | http://psasir.upm.edu.my/id/eprint/87137/1/PGP%209.5-%20immunoreactivity.pdf |
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