Platelet aggregation pattern on light transmission aggregometry among Malaysian healthy individuals
Introduction: Platelet aggregation test using light transmission aggregometry (LTA) is considered as the gold standard for evaluation of platelet function. Variations of platelet aggregation had been reported in apparently healthy individuals whereby a normal cut–off value established locally is h...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Medicine and Health Sciences, University Putra Malaysia
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87203/1/2020110610554905_2020_0236.pdf |
| _version_ | 1825952333634207744 |
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| author | Muhsin, Azlina Mohd Tohit, Eusni Rahayu Md Noor, Sabariah Abdul Karim, Faraizah |
| author_facet | Muhsin, Azlina Mohd Tohit, Eusni Rahayu Md Noor, Sabariah Abdul Karim, Faraizah |
| author_sort | Muhsin, Azlina |
| collection | UPM |
| description | Introduction: Platelet aggregation test using light transmission aggregometry (LTA) is considered as the gold
standard for evaluation of platelet function. Variations of platelet aggregation had been reported in apparently
healthy individuals whereby a normal cut–off value established locally is highly recommended. This study aims
to determine the platelet aggregation pattern and the preliminary findings on reference values for
multiple agonists–induced platelet aggregation among Malaysian healthy individuals in a single centre.
Method: A total number of 63 informed consented healthy individuals consisted of Malay, Chinese and Indian
were recruited among staff and blood donors at National Blood Centre, Kuala Lumpur. Platelet aggregation was
measured using LTA against adenosine diphosphate 10 µM (ADP10), collagen 0.19 mg/mL (COL), ristocetin 1.5
mg/mL (RIS), arachidonic acid 1 mM (AA) and epinephrine 10 µM (EPI). Results were expressed as percent final
aggregation (%FA). Reference values were calculated from mean±2SD. Results: Age, gender and ethnic groups had
no significant effect on platelet aggregation. A variability of platelet aggregation response to EPI was observed among
the healthy individuals. Ten of 33 respondents (30%) had impaired aggregation with <20% FA in response to EPI.
The local population showed a slightly higher aggregation pattern in response to COL, RIS, AA and EPI (excluding non-responders) compared to manufacturer’s reference values. Conclusion: This study has provided a glimpse
of the aggregation pattern of the local nationality showing considerable differences in the reference values from
manufacturer’s; thus highlighting the need of establishing local reference values. |
| first_indexed | 2024-03-06T10:43:12Z |
| format | Article |
| id | upm.eprints-87203 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:43:12Z |
| publishDate | 2020 |
| publisher | Faculty of Medicine and Health Sciences, University Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-872032022-01-13T09:23:25Z http://psasir.upm.edu.my/id/eprint/87203/ Platelet aggregation pattern on light transmission aggregometry among Malaysian healthy individuals Muhsin, Azlina Mohd Tohit, Eusni Rahayu Md Noor, Sabariah Abdul Karim, Faraizah Introduction: Platelet aggregation test using light transmission aggregometry (LTA) is considered as the gold standard for evaluation of platelet function. Variations of platelet aggregation had been reported in apparently healthy individuals whereby a normal cut–off value established locally is highly recommended. This study aims to determine the platelet aggregation pattern and the preliminary findings on reference values for multiple agonists–induced platelet aggregation among Malaysian healthy individuals in a single centre. Method: A total number of 63 informed consented healthy individuals consisted of Malay, Chinese and Indian were recruited among staff and blood donors at National Blood Centre, Kuala Lumpur. Platelet aggregation was measured using LTA against adenosine diphosphate 10 µM (ADP10), collagen 0.19 mg/mL (COL), ristocetin 1.5 mg/mL (RIS), arachidonic acid 1 mM (AA) and epinephrine 10 µM (EPI). Results were expressed as percent final aggregation (%FA). Reference values were calculated from mean±2SD. Results: Age, gender and ethnic groups had no significant effect on platelet aggregation. A variability of platelet aggregation response to EPI was observed among the healthy individuals. Ten of 33 respondents (30%) had impaired aggregation with <20% FA in response to EPI. The local population showed a slightly higher aggregation pattern in response to COL, RIS, AA and EPI (excluding non-responders) compared to manufacturer’s reference values. Conclusion: This study has provided a glimpse of the aggregation pattern of the local nationality showing considerable differences in the reference values from manufacturer’s; thus highlighting the need of establishing local reference values. Faculty of Medicine and Health Sciences, University Putra Malaysia 2020-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87203/1/2020110610554905_2020_0236.pdf Muhsin, Azlina and Mohd Tohit, Eusni Rahayu and Md Noor, Sabariah and Abdul Karim, Faraizah (2020) Platelet aggregation pattern on light transmission aggregometry among Malaysian healthy individuals. Malaysian Journal of Medicine and Health Sciences, 16 (suppl. 9). 33 - 37. ISSN 2636-9346 https://medic.upm.edu.my/upload/dokumen/2020110610554905_2020_0236.pdf |
| spellingShingle | Muhsin, Azlina Mohd Tohit, Eusni Rahayu Md Noor, Sabariah Abdul Karim, Faraizah Platelet aggregation pattern on light transmission aggregometry among Malaysian healthy individuals |
| title | Platelet aggregation pattern on light transmission aggregometry among Malaysian healthy individuals |
| title_full | Platelet aggregation pattern on light transmission aggregometry among Malaysian healthy individuals |
| title_fullStr | Platelet aggregation pattern on light transmission aggregometry among Malaysian healthy individuals |
| title_full_unstemmed | Platelet aggregation pattern on light transmission aggregometry among Malaysian healthy individuals |
| title_short | Platelet aggregation pattern on light transmission aggregometry among Malaysian healthy individuals |
| title_sort | platelet aggregation pattern on light transmission aggregometry among malaysian healthy individuals |
| url | http://psasir.upm.edu.my/id/eprint/87203/1/2020110610554905_2020_0236.pdf |
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