Possible participation of ionotropic glutamate receptors and l-arginine-nitric oxide-cyclic guanosine monophosphate-ATP-Sensitive K+ channel pathway in the antinociceptive activity of cardamonin in acute pain animal models
The perception of pain caused by inflammation serves as a warning sign to avoid further injury. The generation and transmission of pain impulses involves various pathways and receptors. Cardamonin isolated from Boesenbergia rotunda (L.) Mansf. has been reported to exert antinociceptive effects in th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87215/1/Possible%20participation%20of%20ionotropic%20glutamate.pdf |
| _version_ | 1825952336026009600 |
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| author | Chung, Pui Ping Akhtar, Muhammad Nadeem Israf, Daud Ahmad Perimal, Enoch Kumar Sulaiman, Mohd Roslan |
| author_facet | Chung, Pui Ping Akhtar, Muhammad Nadeem Israf, Daud Ahmad Perimal, Enoch Kumar Sulaiman, Mohd Roslan |
| author_sort | Chung, Pui Ping |
| collection | UPM |
| description | The perception of pain caused by inflammation serves as a warning sign to avoid further injury. The generation and transmission of pain impulses involves various pathways and receptors. Cardamonin isolated from Boesenbergia rotunda (L.) Mansf. has been reported to exert antinociceptive effects in thermal and mechanical pain models; however, the precise mechanism has yet to be examined. The present study investigated the possible mechanisms involved in the antinociceptive activity of cardamonin on protein kinase C, N-methyl-d-aspartate (NMDA) and non-NMDA glutamate receptors, l-arginine/cyclic guanosine monophosphate (cGMP) mechanism, as well as the ATP-sensitive potassium (K+) channel. Cardamonin was administered to the animals intra-peritoneally. Present findings showed that cardamonin significantly inhibited pain elicited by intraplantar injection of phorbol 12-myristate 13-acetate (PMA, a protein kinase C activator) with calculated mean ED50 of 2.0 mg/kg (0.9–4.5 mg/kg). The study presented that pre-treatment with MK-801 (NMDA receptor antagonist) and NBQX (non-NMDA receptor antagonist) significantly modulates the antinociceptive activity of cardamonin at 3 mg/kg when tested with glutamate-induced paw licking test. Pre-treatment with l-arginine (a nitric oxide precursor), ODQ (selective inhibitor of soluble guanylyl cyclase) and glibenclamide (ATP-sensitive K+ channel inhibitor) significantly enhanced the antinociception produced by cardamonin. In conclusion, the present findings showed that the antinociceptive activity of cardamonin might involve the modulation of PKC activity, NMDA and non-NMDA glutamate receptors, l-arginine/nitric oxide/cGMP pathway and ATP-sensitive K+ channel. |
| first_indexed | 2024-03-06T10:43:13Z |
| format | Article |
| id | upm.eprints-87215 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:43:13Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-872152022-01-19T02:57:50Z http://psasir.upm.edu.my/id/eprint/87215/ Possible participation of ionotropic glutamate receptors and l-arginine-nitric oxide-cyclic guanosine monophosphate-ATP-Sensitive K+ channel pathway in the antinociceptive activity of cardamonin in acute pain animal models Chung, Pui Ping Akhtar, Muhammad Nadeem Israf, Daud Ahmad Perimal, Enoch Kumar Sulaiman, Mohd Roslan The perception of pain caused by inflammation serves as a warning sign to avoid further injury. The generation and transmission of pain impulses involves various pathways and receptors. Cardamonin isolated from Boesenbergia rotunda (L.) Mansf. has been reported to exert antinociceptive effects in thermal and mechanical pain models; however, the precise mechanism has yet to be examined. The present study investigated the possible mechanisms involved in the antinociceptive activity of cardamonin on protein kinase C, N-methyl-d-aspartate (NMDA) and non-NMDA glutamate receptors, l-arginine/cyclic guanosine monophosphate (cGMP) mechanism, as well as the ATP-sensitive potassium (K+) channel. Cardamonin was administered to the animals intra-peritoneally. Present findings showed that cardamonin significantly inhibited pain elicited by intraplantar injection of phorbol 12-myristate 13-acetate (PMA, a protein kinase C activator) with calculated mean ED50 of 2.0 mg/kg (0.9–4.5 mg/kg). The study presented that pre-treatment with MK-801 (NMDA receptor antagonist) and NBQX (non-NMDA receptor antagonist) significantly modulates the antinociceptive activity of cardamonin at 3 mg/kg when tested with glutamate-induced paw licking test. Pre-treatment with l-arginine (a nitric oxide precursor), ODQ (selective inhibitor of soluble guanylyl cyclase) and glibenclamide (ATP-sensitive K+ channel inhibitor) significantly enhanced the antinociception produced by cardamonin. In conclusion, the present findings showed that the antinociceptive activity of cardamonin might involve the modulation of PKC activity, NMDA and non-NMDA glutamate receptors, l-arginine/nitric oxide/cGMP pathway and ATP-sensitive K+ channel. Multidisciplinary Digital Publishing Institute 2020-11-18 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87215/1/Possible%20participation%20of%20ionotropic%20glutamate.pdf Chung, Pui Ping and Akhtar, Muhammad Nadeem and Israf, Daud Ahmad and Perimal, Enoch Kumar and Sulaiman, Mohd Roslan (2020) Possible participation of ionotropic glutamate receptors and l-arginine-nitric oxide-cyclic guanosine monophosphate-ATP-Sensitive K+ channel pathway in the antinociceptive activity of cardamonin in acute pain animal models. Molecules, 25 (22). art. no. 5385. pp. 1-19. ISSN 1420-3049 https://www.mdpi.com/1420-3049/25/22/5385 10.3390/molecules25225385 |
| spellingShingle | Chung, Pui Ping Akhtar, Muhammad Nadeem Israf, Daud Ahmad Perimal, Enoch Kumar Sulaiman, Mohd Roslan Possible participation of ionotropic glutamate receptors and l-arginine-nitric oxide-cyclic guanosine monophosphate-ATP-Sensitive K+ channel pathway in the antinociceptive activity of cardamonin in acute pain animal models |
| title | Possible participation of ionotropic glutamate receptors and l-arginine-nitric oxide-cyclic guanosine monophosphate-ATP-Sensitive K+ channel pathway in the antinociceptive activity of cardamonin in acute pain animal models |
| title_full | Possible participation of ionotropic glutamate receptors and l-arginine-nitric oxide-cyclic guanosine monophosphate-ATP-Sensitive K+ channel pathway in the antinociceptive activity of cardamonin in acute pain animal models |
| title_fullStr | Possible participation of ionotropic glutamate receptors and l-arginine-nitric oxide-cyclic guanosine monophosphate-ATP-Sensitive K+ channel pathway in the antinociceptive activity of cardamonin in acute pain animal models |
| title_full_unstemmed | Possible participation of ionotropic glutamate receptors and l-arginine-nitric oxide-cyclic guanosine monophosphate-ATP-Sensitive K+ channel pathway in the antinociceptive activity of cardamonin in acute pain animal models |
| title_short | Possible participation of ionotropic glutamate receptors and l-arginine-nitric oxide-cyclic guanosine monophosphate-ATP-Sensitive K+ channel pathway in the antinociceptive activity of cardamonin in acute pain animal models |
| title_sort | possible participation of ionotropic glutamate receptors and l arginine nitric oxide cyclic guanosine monophosphate atp sensitive k channel pathway in the antinociceptive activity of cardamonin in acute pain animal models |
| url | http://psasir.upm.edu.my/id/eprint/87215/1/Possible%20participation%20of%20ionotropic%20glutamate.pdf |
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