Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats
The current study evaluated the γ-aminobutyric acid (GABA) producing ability from three novel strains of lactic acid bacteria (L. plantarum Taj-Apis362, assigned as UPMC90, UPMC91, and UPMC1065) co-cultured with starter culture in a yogurt. A combination of UPMC90 + UPMC91 with starter culture symbi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87231/1/Potentiality%20of%20self-cloned%20Lactobacillus.pdf |
| _version_ | 1825952338796347392 |
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| author | Hussin, Farah Salina Chay, Shyan Yea Zarei, Mohammad Meor Hussin, Anis Shobirin Wan Ibadullah, Wan Zunairah Zaharuddin, Nurul Dhania Wazir, Hazrati Saari, Nazamid |
| author_facet | Hussin, Farah Salina Chay, Shyan Yea Zarei, Mohammad Meor Hussin, Anis Shobirin Wan Ibadullah, Wan Zunairah Zaharuddin, Nurul Dhania Wazir, Hazrati Saari, Nazamid |
| author_sort | Hussin, Farah Salina |
| collection | UPM |
| description | The current study evaluated the γ-aminobutyric acid (GABA) producing ability from three novel strains of lactic acid bacteria (L. plantarum Taj-Apis362, assigned as UPMC90, UPMC91, and UPMC1065) co-cultured with starter culture in a yogurt. A combination of UPMC90 + UPMC91 with starter culture symbiotically revealed the most prominent GABA-producing effect. Response surface methodology revealed the optimized fermentation conditions at 39.0 °C, 7.25 h, and 11.5 mM glutamate substrate concentration to produce GABA-rich yogurt (29.96 mg/100 g) with desirable pH (3.93) and water-holding capacity (63.06%). At 2% glucose to replace pyridoxal-5-phosphate (PLP), a cofactor typically needed during GABA production, GABA content was further enhanced to 59.00 mg/100 g. In vivo study using this sample revealed a blood pressure-lowering efficacy at 0.1 mg/kg GABA dosage (equivalent to 30 mg/kg GABA-rich yogurt) in spontaneously hypertensive rats. An improved method to produce GABA-rich yogurt has been established, involving shorter fermentation time and lower glutamate concentration than previous work, along with glucose induction that omits the use of costly PLP, fostering the potential of developing a GABA-rich functional dairy product through natural fermentation with desirable product quality and antihypertensive property. |
| first_indexed | 2024-03-06T10:43:16Z |
| format | Article |
| id | upm.eprints-87231 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:43:16Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-872312022-01-20T04:32:32Z http://psasir.upm.edu.my/id/eprint/87231/ Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats Hussin, Farah Salina Chay, Shyan Yea Zarei, Mohammad Meor Hussin, Anis Shobirin Wan Ibadullah, Wan Zunairah Zaharuddin, Nurul Dhania Wazir, Hazrati Saari, Nazamid The current study evaluated the γ-aminobutyric acid (GABA) producing ability from three novel strains of lactic acid bacteria (L. plantarum Taj-Apis362, assigned as UPMC90, UPMC91, and UPMC1065) co-cultured with starter culture in a yogurt. A combination of UPMC90 + UPMC91 with starter culture symbiotically revealed the most prominent GABA-producing effect. Response surface methodology revealed the optimized fermentation conditions at 39.0 °C, 7.25 h, and 11.5 mM glutamate substrate concentration to produce GABA-rich yogurt (29.96 mg/100 g) with desirable pH (3.93) and water-holding capacity (63.06%). At 2% glucose to replace pyridoxal-5-phosphate (PLP), a cofactor typically needed during GABA production, GABA content was further enhanced to 59.00 mg/100 g. In vivo study using this sample revealed a blood pressure-lowering efficacy at 0.1 mg/kg GABA dosage (equivalent to 30 mg/kg GABA-rich yogurt) in spontaneously hypertensive rats. An improved method to produce GABA-rich yogurt has been established, involving shorter fermentation time and lower glutamate concentration than previous work, along with glucose induction that omits the use of costly PLP, fostering the potential of developing a GABA-rich functional dairy product through natural fermentation with desirable product quality and antihypertensive property. Multidisciplinary Digital Publishing Institute 2020-12-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87231/1/Potentiality%20of%20self-cloned%20Lactobacillus.pdf Hussin, Farah Salina and Chay, Shyan Yea and Zarei, Mohammad and Meor Hussin, Anis Shobirin and Wan Ibadullah, Wan Zunairah and Zaharuddin, Nurul Dhania and Wazir, Hazrati and Saari, Nazamid (2020) Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats. Foods, 9 (12). pp. 1-15. ISSN 2304-8158 https://www.mdpi.com/2304-8158/9/12/1826 10.3390/foods9121826 |
| spellingShingle | Hussin, Farah Salina Chay, Shyan Yea Zarei, Mohammad Meor Hussin, Anis Shobirin Wan Ibadullah, Wan Zunairah Zaharuddin, Nurul Dhania Wazir, Hazrati Saari, Nazamid Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats |
| title | Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats |
| title_full | Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats |
| title_fullStr | Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats |
| title_full_unstemmed | Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats |
| title_short | Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats |
| title_sort | potentiality of self cloned lactobacillus plantarum taj apis362 for enhancing gaba production in yogurt under glucose induction optimization and its cardiovascular effect on spontaneous hypertensive rats |
| url | http://psasir.upm.edu.my/id/eprint/87231/1/Potentiality%20of%20self-cloned%20Lactobacillus.pdf |
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