Prevalence of methicillin resistant staphylococcus aureus in raw goat milks from selected farms in Terengganu, Malaysia
The emergence of antimicrobial drug resistant bacteria has been a concern worldwide. One of bacteria that has been reported to develop resistance is Methicillin Resistant Staphylococcus aureus (MRSA). Recent studies showed that Livestock Associated MRSA (LA-MRSA) was found...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Institut Pertanian Bogor,Bogor Agricultural University
2020
|
| _version_ | 1825952346987823104 |
|---|---|
| author | Chai, M. H. M. Faiq, T. A. Ariffin, S. M. Z. Suhaili, Z. Sukiman, M. Z. Ghazali, M. F. |
| author_facet | Chai, M. H. M. Faiq, T. A. Ariffin, S. M. Z. Suhaili, Z. Sukiman, M. Z. Ghazali, M. F. |
| author_sort | Chai, M. H. |
| collection | UPM |
| description | The emergence of antimicrobial drug resistant bacteria has been a concern worldwide. One of bacteria that has been reported to develop resistance is Methicillin Resistant Staphylococcus aureus (MRSA). Recent studies showed that Livestock Associated MRSA (LA-MRSA) was found in domestic food animals and their handlers. The aim of this study was to investigate the prevalence of MRSA in goat milk of goat farms located in Terengganu. A total of 664 udder milks were taken from 332 goats at 40 selected farms within Terengganu state. Then, screening of bacteria and isolation of suspected S. aureus isolates in the milk samples was done using selective agar, Gram staining and biochemical tests. The identity of the bacteria isolated was further confirmed using PCR where specific designed primers were used to detect the presence of nuc gene of S. aureus (278bp) and mecAgene (533bp) of MRSA. Both S. aureus and MRSA isolateswere also tested for their susceptibilities toward the antimicrobial drugs. Fifty milk samples were found to contain S. aureus and one of the S. aureus isolates were MRSA. The bacteria isolates were found to have higher tendency to be resistance toward Penicillin (26.0%) and Oxacillin (12.0%). This study provides useful data on the current status of MRSA prevalence in small ruminant’s milk, which can be used to prevent transmission of LA-MRSA to human and other animals. |
| first_indexed | 2024-03-06T10:43:23Z |
| format | Article |
| id | upm.eprints-87281 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:43:23Z |
| publishDate | 2020 |
| publisher | Institut Pertanian Bogor,Bogor Agricultural University |
| record_format | dspace |
| spelling | upm.eprints-872812023-07-18T01:41:54Z http://psasir.upm.edu.my/id/eprint/87281/ Prevalence of methicillin resistant staphylococcus aureus in raw goat milks from selected farms in Terengganu, Malaysia Chai, M. H. M. Faiq, T. A. Ariffin, S. M. Z. Suhaili, Z. Sukiman, M. Z. Ghazali, M. F. The emergence of antimicrobial drug resistant bacteria has been a concern worldwide. One of bacteria that has been reported to develop resistance is Methicillin Resistant Staphylococcus aureus (MRSA). Recent studies showed that Livestock Associated MRSA (LA-MRSA) was found in domestic food animals and their handlers. The aim of this study was to investigate the prevalence of MRSA in goat milk of goat farms located in Terengganu. A total of 664 udder milks were taken from 332 goats at 40 selected farms within Terengganu state. Then, screening of bacteria and isolation of suspected S. aureus isolates in the milk samples was done using selective agar, Gram staining and biochemical tests. The identity of the bacteria isolated was further confirmed using PCR where specific designed primers were used to detect the presence of nuc gene of S. aureus (278bp) and mecAgene (533bp) of MRSA. Both S. aureus and MRSA isolateswere also tested for their susceptibilities toward the antimicrobial drugs. Fifty milk samples were found to contain S. aureus and one of the S. aureus isolates were MRSA. The bacteria isolates were found to have higher tendency to be resistance toward Penicillin (26.0%) and Oxacillin (12.0%). This study provides useful data on the current status of MRSA prevalence in small ruminant’s milk, which can be used to prevent transmission of LA-MRSA to human and other animals. Institut Pertanian Bogor,Bogor Agricultural University 2020-03 Article PeerReviewed Chai, M. H. and M. Faiq, T. A. and Ariffin, S. M. Z. and Suhaili, Z. and Sukiman, M. Z. and Ghazali, M. F. (2020) Prevalence of methicillin resistant staphylococcus aureus in raw goat milks from selected farms in Terengganu, Malaysia. Tropical Animal Science Journal, 43 (1). 64 - 69. ISSN 2615-787X ; E: 2615-790X http://journal.ipb.ac.id/index.php/tasj 0.5398/tasj.2020.43.1.64 |
| spellingShingle | Chai, M. H. M. Faiq, T. A. Ariffin, S. M. Z. Suhaili, Z. Sukiman, M. Z. Ghazali, M. F. Prevalence of methicillin resistant staphylococcus aureus in raw goat milks from selected farms in Terengganu, Malaysia |
| title | Prevalence of methicillin resistant staphylococcus aureus in raw goat milks from selected farms in Terengganu, Malaysia |
| title_full | Prevalence of methicillin resistant staphylococcus aureus in raw goat milks from selected farms in Terengganu, Malaysia |
| title_fullStr | Prevalence of methicillin resistant staphylococcus aureus in raw goat milks from selected farms in Terengganu, Malaysia |
| title_full_unstemmed | Prevalence of methicillin resistant staphylococcus aureus in raw goat milks from selected farms in Terengganu, Malaysia |
| title_short | Prevalence of methicillin resistant staphylococcus aureus in raw goat milks from selected farms in Terengganu, Malaysia |
| title_sort | prevalence of methicillin resistant staphylococcus aureus in raw goat milks from selected farms in terengganu malaysia |
| work_keys_str_mv | AT chaimh prevalenceofmethicillinresistantstaphylococcusaureusinrawgoatmilksfromselectedfarmsinterengganumalaysia AT mfaiqta prevalenceofmethicillinresistantstaphylococcusaureusinrawgoatmilksfromselectedfarmsinterengganumalaysia AT ariffinsmz prevalenceofmethicillinresistantstaphylococcusaureusinrawgoatmilksfromselectedfarmsinterengganumalaysia AT suhailiz prevalenceofmethicillinresistantstaphylococcusaureusinrawgoatmilksfromselectedfarmsinterengganumalaysia AT sukimanmz prevalenceofmethicillinresistantstaphylococcusaureusinrawgoatmilksfromselectedfarmsinterengganumalaysia AT ghazalimf prevalenceofmethicillinresistantstaphylococcusaureusinrawgoatmilksfromselectedfarmsinterengganumalaysia |