Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts
Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2020
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/87346/1/Protein%20expression%20patterns%20in%20HEK-.pdf |
| _version_ | 1796981840945348608 |
|---|---|
| author | Yang, Shun Kai Leo, Teik Kee Mai, Chun Wai Chin, Swee Yee Lamasudin, Dhilia Udie Lim, Erin Swee Hua Tan, Ngai Paing Lai, Kok Song |
| author_facet | Yang, Shun Kai Leo, Teik Kee Mai, Chun Wai Chin, Swee Yee Lamasudin, Dhilia Udie Lim, Erin Swee Hua Tan, Ngai Paing Lai, Kok Song |
| author_sort | Yang, Shun Kai |
| collection | UPM |
| description | Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two-dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK-Blue™-4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK-Blue™-4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin-1 was also upregulated in HEK-Blue™-4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK-Blue™-4 cells and induced the expression of peroxiredoxin-1, which protects the cells from reactive oxygen species during the inflammation process. |
| first_indexed | 2024-03-06T10:43:32Z |
| format | Article |
| id | upm.eprints-87346 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:43:32Z |
| publishDate | 2020 |
| publisher | Wolters Kluwer |
| record_format | dspace |
| spelling | upm.eprints-873462022-11-07T09:09:04Z http://psasir.upm.edu.my/id/eprint/87346/ Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts Yang, Shun Kai Leo, Teik Kee Mai, Chun Wai Chin, Swee Yee Lamasudin, Dhilia Udie Lim, Erin Swee Hua Tan, Ngai Paing Lai, Kok Song Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two-dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK-Blue™-4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK-Blue™-4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin-1 was also upregulated in HEK-Blue™-4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK-Blue™-4 cells and induced the expression of peroxiredoxin-1, which protects the cells from reactive oxygen species during the inflammation process. Wolters Kluwer 2020-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87346/1/Protein%20expression%20patterns%20in%20HEK-.pdf Yang, Shun Kai and Leo, Teik Kee and Mai, Chun Wai and Chin, Swee Yee and Lamasudin, Dhilia Udie and Lim, Erin Swee Hua and Tan, Ngai Paing and Lai, Kok Song (2020) Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts. Pharmacognosy Magazine, 70 (16). 431 - 434. ISSN 0973-1296; ESSN: 0976-4062 https://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=70;spage=431;epage=434;aulast=Yang;type=0 10.4103/pm.pm_281_19 |
| spellingShingle | Yang, Shun Kai Leo, Teik Kee Mai, Chun Wai Chin, Swee Yee Lamasudin, Dhilia Udie Lim, Erin Swee Hua Tan, Ngai Paing Lai, Kok Song Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts |
| title | Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts |
| title_full | Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts |
| title_fullStr | Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts |
| title_full_unstemmed | Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts |
| title_short | Protein expression patterns in HEK-blue-cells treated with Clinacanthus nutans extracts |
| title_sort | protein expression patterns in hek blue cells treated with clinacanthus nutans extracts |
| url | http://psasir.upm.edu.my/id/eprint/87346/1/Protein%20expression%20patterns%20in%20HEK-.pdf |
| work_keys_str_mv | AT yangshunkai proteinexpressionpatternsinhekbluecellstreatedwithclinacanthusnutansextracts AT leoteikkee proteinexpressionpatternsinhekbluecellstreatedwithclinacanthusnutansextracts AT maichunwai proteinexpressionpatternsinhekbluecellstreatedwithclinacanthusnutansextracts AT chinsweeyee proteinexpressionpatternsinhekbluecellstreatedwithclinacanthusnutansextracts AT lamasudindhiliaudie proteinexpressionpatternsinhekbluecellstreatedwithclinacanthusnutansextracts AT limerinsweehua proteinexpressionpatternsinhekbluecellstreatedwithclinacanthusnutansextracts AT tanngaipaing proteinexpressionpatternsinhekbluecellstreatedwithclinacanthusnutansextracts AT laikoksong proteinexpressionpatternsinhekbluecellstreatedwithclinacanthusnutansextracts |