Screening of medium constituents for the cultivation of Scenedesmus dimorphus UTEX 1237 using 2k factorial design approach

Freshwater microalga, Scenedesmus dimorphus is a beneficial natural resource for producing biofuel and novel bioproducts. S. dimorphus growth greatly depends on physical culture conditions as well as medium constituents. A study was conducted to select the suitable medium components that could promo...

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Main Authors: Ravindran, Bavistra, Kassim, Mohd Asyraf, Mohamed, Mohd Shamzi
Format: Article
Published: Institute of Physics Publishing 2020
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author Ravindran, Bavistra
Kassim, Mohd Asyraf
Mohamed, Mohd Shamzi
author_facet Ravindran, Bavistra
Kassim, Mohd Asyraf
Mohamed, Mohd Shamzi
author_sort Ravindran, Bavistra
collection UPM
description Freshwater microalga, Scenedesmus dimorphus is a beneficial natural resource for producing biofuel and novel bioproducts. S. dimorphus growth greatly depends on physical culture conditions as well as medium constituents. A study was conducted to select the suitable medium components that could promote high biomass production in S. dimorphus UTEX 1237. Screening of growth medium constituents was performed using the two-level factorial design (2k FD) method. S. dimorphus was cultivated in TAPS aquaculture medium employing two types of carbon source (sodium bicarbonate and acetic acid) and three types of nitrogen sources (ammonium chloride, sodium nitrate, and urea). Consequently, medium components that significantly influenced biomass production comprised of acetic acid, ammonium chloride, and urea. Two weeks of S. dimorphus cultivation in shake flask system consisting of 1.5 g/L of acetic acid, 10 g/L of ammonium chloride and 0.4 g/L of urea was found to affect a specific growth rate of 0.0049 h−1 and biomass productivity of 0.05 g/L/day, thus affecting 66.7% improvement over normal TAP formulation.
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spelling upm.eprints-875372022-11-23T02:30:08Z http://psasir.upm.edu.my/id/eprint/87537/ Screening of medium constituents for the cultivation of Scenedesmus dimorphus UTEX 1237 using 2k factorial design approach Ravindran, Bavistra Kassim, Mohd Asyraf Mohamed, Mohd Shamzi Freshwater microalga, Scenedesmus dimorphus is a beneficial natural resource for producing biofuel and novel bioproducts. S. dimorphus growth greatly depends on physical culture conditions as well as medium constituents. A study was conducted to select the suitable medium components that could promote high biomass production in S. dimorphus UTEX 1237. Screening of growth medium constituents was performed using the two-level factorial design (2k FD) method. S. dimorphus was cultivated in TAPS aquaculture medium employing two types of carbon source (sodium bicarbonate and acetic acid) and three types of nitrogen sources (ammonium chloride, sodium nitrate, and urea). Consequently, medium components that significantly influenced biomass production comprised of acetic acid, ammonium chloride, and urea. Two weeks of S. dimorphus cultivation in shake flask system consisting of 1.5 g/L of acetic acid, 10 g/L of ammonium chloride and 0.4 g/L of urea was found to affect a specific growth rate of 0.0049 h−1 and biomass productivity of 0.05 g/L/day, thus affecting 66.7% improvement over normal TAP formulation. Institute of Physics Publishing 2020 Article PeerReviewed Ravindran, Bavistra and Kassim, Mohd Asyraf and Mohamed, Mohd Shamzi (2020) Screening of medium constituents for the cultivation of Scenedesmus dimorphus UTEX 1237 using 2k factorial design approach. IOP Conference Series: Materials Science and Engineering, 716. pp. 1-8. ISSN 1757-8981 https://iopscience.iop.org/article/10.1088/1757-899X/716/1/012003
spellingShingle Ravindran, Bavistra
Kassim, Mohd Asyraf
Mohamed, Mohd Shamzi
Screening of medium constituents for the cultivation of Scenedesmus dimorphus UTEX 1237 using 2k factorial design approach
title Screening of medium constituents for the cultivation of Scenedesmus dimorphus UTEX 1237 using 2k factorial design approach
title_full Screening of medium constituents for the cultivation of Scenedesmus dimorphus UTEX 1237 using 2k factorial design approach
title_fullStr Screening of medium constituents for the cultivation of Scenedesmus dimorphus UTEX 1237 using 2k factorial design approach
title_full_unstemmed Screening of medium constituents for the cultivation of Scenedesmus dimorphus UTEX 1237 using 2k factorial design approach
title_short Screening of medium constituents for the cultivation of Scenedesmus dimorphus UTEX 1237 using 2k factorial design approach
title_sort screening of medium constituents for the cultivation of scenedesmus dimorphus utex 1237 using 2k factorial design approach
work_keys_str_mv AT ravindranbavistra screeningofmediumconstituentsforthecultivationofscenedesmusdimorphusutex1237using2kfactorialdesignapproach
AT kassimmohdasyraf screeningofmediumconstituentsforthecultivationofscenedesmusdimorphusutex1237using2kfactorialdesignapproach
AT mohamedmohdshamzi screeningofmediumconstituentsforthecultivationofscenedesmusdimorphusutex1237using2kfactorialdesignapproach