A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol
A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilizati...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87683/1/ABSTRACT.pdf |
| _version_ | 1796981874985271296 |
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| author | Mat Zaid, Mohd Hazani Abdullah, Jaafar Rozi, Normazida Mohamad Rozlan, Aliff Aiman Abu Hanifah, Sharina |
| author_facet | Mat Zaid, Mohd Hazani Abdullah, Jaafar Rozi, Normazida Mohamad Rozlan, Aliff Aiman Abu Hanifah, Sharina |
| author_sort | Mat Zaid, Mohd Hazani |
| collection | UPM |
| description | A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilization of 76-mer aptamer probe. The morphology and structure of the nanomaterial were characterized by UV-visible absorption spectra, Fluorescence spectra, Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Moreover, cyclic voltammetry and electrochemical impedance spectroscopy were used to investigate the electrochemical performance of the prepared electrodes. Subsequently, impedimetric (EIS) measurements were employed to investigate the relative impedances changes before and after E2 binding, which results in a linear relationship of E2 concentration in the range of 1.0 × 10−7 to 1.0 × 10 −12 M, with a detection limit of 0.5 × 10−12 M. Moreover, the developed biosensor showed high selectivity toward E2 and exhibited excellent discrimination against progesterone (PRG), estriol (E3) and bisphenol A (BPA), respectively. Moreover, the average recovery rate of spiked river water samples with E2 ranged from 98.2% to 103.8%, with relative standard deviations between 1.1% and 3.8%, revealing the potential application of the present biosensor for E2 detection in water samples. |
| first_indexed | 2024-03-06T10:44:02Z |
| format | Article |
| id | upm.eprints-87683 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:44:02Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-876832022-07-06T03:48:20Z http://psasir.upm.edu.my/id/eprint/87683/ A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol Mat Zaid, Mohd Hazani Abdullah, Jaafar Rozi, Normazida Mohamad Rozlan, Aliff Aiman Abu Hanifah, Sharina A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilization of 76-mer aptamer probe. The morphology and structure of the nanomaterial were characterized by UV-visible absorption spectra, Fluorescence spectra, Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Moreover, cyclic voltammetry and electrochemical impedance spectroscopy were used to investigate the electrochemical performance of the prepared electrodes. Subsequently, impedimetric (EIS) measurements were employed to investigate the relative impedances changes before and after E2 binding, which results in a linear relationship of E2 concentration in the range of 1.0 × 10−7 to 1.0 × 10 −12 M, with a detection limit of 0.5 × 10−12 M. Moreover, the developed biosensor showed high selectivity toward E2 and exhibited excellent discrimination against progesterone (PRG), estriol (E3) and bisphenol A (BPA), respectively. Moreover, the average recovery rate of spiked river water samples with E2 ranged from 98.2% to 103.8%, with relative standard deviations between 1.1% and 3.8%, revealing the potential application of the present biosensor for E2 detection in water samples. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87683/1/ABSTRACT.pdf Mat Zaid, Mohd Hazani and Abdullah, Jaafar and Rozi, Normazida and Mohamad Rozlan, Aliff Aiman and Abu Hanifah, Sharina (2020) A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol. Nanomaterials, 10 (7). pp. 1-14. ISSN 2079-4991 https://www.mdpi.com/2079-4991/10/7/1346 10.3390/nano10071346 |
| spellingShingle | Mat Zaid, Mohd Hazani Abdullah, Jaafar Rozi, Normazida Mohamad Rozlan, Aliff Aiman Abu Hanifah, Sharina A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol |
| title | A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol |
| title_full | A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol |
| title_fullStr | A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol |
| title_full_unstemmed | A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol |
| title_short | A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol |
| title_sort | sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ss estradiol |
| url | http://psasir.upm.edu.my/id/eprint/87683/1/ABSTRACT.pdf |
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