Advances in the diagnosis of foot-and-mouth disease
Foot-and-mouth disease (FMD) is a devastating livestock disease caused by foot-and-mouth disease virus (FMDV). Outbreaks of this disease in a country always result in conspicuous economic losses to livestock industry and subsequently lead to serious socioeconomic damages due to the immediate imposit...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87777/1/ABSTRACT.pdf |
| _version_ | 1796981886856200192 |
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| author | Wong, Chuan Loo Yong, Chean Yeah Ong, Hui Kian Ho, Kok Lian Tan, Wen Siang |
| author_facet | Wong, Chuan Loo Yong, Chean Yeah Ong, Hui Kian Ho, Kok Lian Tan, Wen Siang |
| author_sort | Wong, Chuan Loo |
| collection | UPM |
| description | Foot-and-mouth disease (FMD) is a devastating livestock disease caused by foot-and-mouth disease virus (FMDV). Outbreaks of this disease in a country always result in conspicuous economic losses to livestock industry and subsequently lead to serious socioeconomic damages due to the immediate imposition of trade embargo. Rapid and accurate diagnoses are imperative to control this infectious virus. In the current review, enzyme-linked immunosorbent assay (ELISA)-based methods used in FMD diagnosis are extensively reviewed, particularly the sandwich, liquid-phase blocking, and solid-phase competition ELISA. The differentiation of infected animals from vaccinated animals using ELISA-based methods is also highlighted, in which the role of 3ABC polyprotein as a marker is reviewed intensively. Recently, more studies are focusing on the molecular diagnostic methods, which detect the viral nucleic acids based on reverse transcription-polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (RT-LAMP). These methods are generally more sensitive because of their ability to amplify a minute amount of the viral nucleic acids. In this digital era, the RT-PCR and RT-LAMP are progressing toward the mobile versions, aiming for on-site FMDV diagnosis. Apart from RT-PCR and RT-LAMP, another diagnostic assay specifically designed for on-site diagnosis is the lateral flow immunochromatographic test strips. These test strips have some distinct advantages over other diagnostic methods, whereby the assay often does not require the aid of an external device, which greatly lowers the cost per test. In addition, the on-site diagnostic test can be easily performed by untrained personnel including farmers, and the results can be obtained in a few minutes. Lastly, the use of FMDV diagnostic assays for progressive control of the disease is also discussed critically. |
| first_indexed | 2024-03-06T10:44:13Z |
| format | Article |
| id | upm.eprints-87777 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:44:13Z |
| publishDate | 2020 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | upm.eprints-877772022-06-14T08:39:04Z http://psasir.upm.edu.my/id/eprint/87777/ Advances in the diagnosis of foot-and-mouth disease Wong, Chuan Loo Yong, Chean Yeah Ong, Hui Kian Ho, Kok Lian Tan, Wen Siang Foot-and-mouth disease (FMD) is a devastating livestock disease caused by foot-and-mouth disease virus (FMDV). Outbreaks of this disease in a country always result in conspicuous economic losses to livestock industry and subsequently lead to serious socioeconomic damages due to the immediate imposition of trade embargo. Rapid and accurate diagnoses are imperative to control this infectious virus. In the current review, enzyme-linked immunosorbent assay (ELISA)-based methods used in FMD diagnosis are extensively reviewed, particularly the sandwich, liquid-phase blocking, and solid-phase competition ELISA. The differentiation of infected animals from vaccinated animals using ELISA-based methods is also highlighted, in which the role of 3ABC polyprotein as a marker is reviewed intensively. Recently, more studies are focusing on the molecular diagnostic methods, which detect the viral nucleic acids based on reverse transcription-polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (RT-LAMP). These methods are generally more sensitive because of their ability to amplify a minute amount of the viral nucleic acids. In this digital era, the RT-PCR and RT-LAMP are progressing toward the mobile versions, aiming for on-site FMDV diagnosis. Apart from RT-PCR and RT-LAMP, another diagnostic assay specifically designed for on-site diagnosis is the lateral flow immunochromatographic test strips. These test strips have some distinct advantages over other diagnostic methods, whereby the assay often does not require the aid of an external device, which greatly lowers the cost per test. In addition, the on-site diagnostic test can be easily performed by untrained personnel including farmers, and the results can be obtained in a few minutes. Lastly, the use of FMDV diagnostic assays for progressive control of the disease is also discussed critically. Frontiers Media 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87777/1/ABSTRACT.pdf Wong, Chuan Loo and Yong, Chean Yeah and Ong, Hui Kian and Ho, Kok Lian and Tan, Wen Siang (2020) Advances in the diagnosis of foot-and-mouth disease. Frontiers in Veterinary Science, 7. pp. 1-24. ISSN 2297-1769 https://www.frontiersin.org/articles/10.3389/fvets.2020.00477/full 10.3389/fvets.2020.00477 |
| spellingShingle | Wong, Chuan Loo Yong, Chean Yeah Ong, Hui Kian Ho, Kok Lian Tan, Wen Siang Advances in the diagnosis of foot-and-mouth disease |
| title | Advances in the diagnosis of foot-and-mouth disease |
| title_full | Advances in the diagnosis of foot-and-mouth disease |
| title_fullStr | Advances in the diagnosis of foot-and-mouth disease |
| title_full_unstemmed | Advances in the diagnosis of foot-and-mouth disease |
| title_short | Advances in the diagnosis of foot-and-mouth disease |
| title_sort | advances in the diagnosis of foot and mouth disease |
| url | http://psasir.upm.edu.my/id/eprint/87777/1/ABSTRACT.pdf |
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