Antibiotic susceptibility and pathogenicity of Aeromonas hydrophila isolated from red hybrid tilapia ( Oreochromis niloticus× Oreochromis mossambicus) in Malaysia
Background and aim: Aeromonas hydrophila is a major cause of bacterial infections affecting a wide range of warm water fishes worldwide. In Malaysia, A. hydrophila isolations from diseased fishes were previously reported; however, with limited information. The present study investigates the antibiot...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Veterinary World
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87890/1/ABSTRACT.pdf |
| _version_ | 1825952426696376320 |
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| author | Ahmad Pauzi, Nurfarahin Mohamad, Nurliyana Sayuti, Mohamad Azzam Md Yasin, Ina Salwany Saad, Mohd Zamri Nasruddin, Nurrul Shaqinah Azmai, Mohammad Noor Amal |
| author_facet | Ahmad Pauzi, Nurfarahin Mohamad, Nurliyana Sayuti, Mohamad Azzam Md Yasin, Ina Salwany Saad, Mohd Zamri Nasruddin, Nurrul Shaqinah Azmai, Mohammad Noor Amal |
| author_sort | Ahmad Pauzi, Nurfarahin |
| collection | UPM |
| description | Background and aim: Aeromonas hydrophila is a major cause of bacterial infections affecting a wide range of warm water fishes worldwide. In Malaysia, A. hydrophila isolations from diseased fishes were previously reported; however, with limited information. The present study investigates the antibiotic susceptibility and pathogenicity of A. hydrophila isolated from farmed red hybrid tilapia (Oreochromis spp.) in Malaysia. Materials and methods: A. hydrophila was biochemically identified and subjected to antibiotic susceptibility tests. The isolate was then intraperitoneally injected into red hybrid tilapia, and the mortality, clinicopathological changes, and LD50 were determined up to 240 h post-infection (hpi).
Results: The isolate demonstrated multiple antibiotic resistances (MAR) toward amikacin, ampicillin, cefotaxime, amoxicillin, trimethoprim-sulfamethoxazole, erythromycin, and streptomycin, with a MAR index of 0.5. The experimental infection of A. hydrophila at 105 CFU/mL in the red hybrid tilapia resulted in 100% mortality at 240 hpi. The LD50 was determined at 1.1×104 CFU/mL. Infected fish demonstrated occasional erratic swimming patterns, localized hemorrhages and depigmentation on the body and operculum areas, fin erosion, enlargement of the gall bladder, and hemorrhage in internal organs. Microscopic observation of infected fish revealed brain congestion, tubular necrosis, and glomerular shrinkage in the kidneys, necrosis of hepatocytes, and congestion of blood vessels in the liver. Conclusion: The high virulence of A. hydrophila to the red hybrid tilapia emphasizes the importance of active, on-going monitoring of its prevalence in Malaysian tilapia farming. |
| first_indexed | 2024-03-06T10:44:27Z |
| format | Article |
| id | upm.eprints-87890 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:44:27Z |
| publishDate | 2020 |
| publisher | Veterinary World |
| record_format | dspace |
| spelling | upm.eprints-878902022-05-24T08:11:17Z http://psasir.upm.edu.my/id/eprint/87890/ Antibiotic susceptibility and pathogenicity of Aeromonas hydrophila isolated from red hybrid tilapia ( Oreochromis niloticus× Oreochromis mossambicus) in Malaysia Ahmad Pauzi, Nurfarahin Mohamad, Nurliyana Sayuti, Mohamad Azzam Md Yasin, Ina Salwany Saad, Mohd Zamri Nasruddin, Nurrul Shaqinah Azmai, Mohammad Noor Amal Background and aim: Aeromonas hydrophila is a major cause of bacterial infections affecting a wide range of warm water fishes worldwide. In Malaysia, A. hydrophila isolations from diseased fishes were previously reported; however, with limited information. The present study investigates the antibiotic susceptibility and pathogenicity of A. hydrophila isolated from farmed red hybrid tilapia (Oreochromis spp.) in Malaysia. Materials and methods: A. hydrophila was biochemically identified and subjected to antibiotic susceptibility tests. The isolate was then intraperitoneally injected into red hybrid tilapia, and the mortality, clinicopathological changes, and LD50 were determined up to 240 h post-infection (hpi). Results: The isolate demonstrated multiple antibiotic resistances (MAR) toward amikacin, ampicillin, cefotaxime, amoxicillin, trimethoprim-sulfamethoxazole, erythromycin, and streptomycin, with a MAR index of 0.5. The experimental infection of A. hydrophila at 105 CFU/mL in the red hybrid tilapia resulted in 100% mortality at 240 hpi. The LD50 was determined at 1.1×104 CFU/mL. Infected fish demonstrated occasional erratic swimming patterns, localized hemorrhages and depigmentation on the body and operculum areas, fin erosion, enlargement of the gall bladder, and hemorrhage in internal organs. Microscopic observation of infected fish revealed brain congestion, tubular necrosis, and glomerular shrinkage in the kidneys, necrosis of hepatocytes, and congestion of blood vessels in the liver. Conclusion: The high virulence of A. hydrophila to the red hybrid tilapia emphasizes the importance of active, on-going monitoring of its prevalence in Malaysian tilapia farming. Veterinary World 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87890/1/ABSTRACT.pdf Ahmad Pauzi, Nurfarahin and Mohamad, Nurliyana and Sayuti, Mohamad Azzam and Md Yasin, Ina Salwany and Saad, Mohd Zamri and Nasruddin, Nurrul Shaqinah and Azmai, Mohammad Noor Amal (2020) Antibiotic susceptibility and pathogenicity of Aeromonas hydrophila isolated from red hybrid tilapia ( Oreochromis niloticus× Oreochromis mossambicus) in Malaysia. Veterinary World, 13 (10). 2166 - 2171. ISSN 0972-8988; ESSN: 2231-0916 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7704323/ 10.14202/vetworld.2020.2166-2171 |
| spellingShingle | Ahmad Pauzi, Nurfarahin Mohamad, Nurliyana Sayuti, Mohamad Azzam Md Yasin, Ina Salwany Saad, Mohd Zamri Nasruddin, Nurrul Shaqinah Azmai, Mohammad Noor Amal Antibiotic susceptibility and pathogenicity of Aeromonas hydrophila isolated from red hybrid tilapia ( Oreochromis niloticus× Oreochromis mossambicus) in Malaysia |
| title | Antibiotic susceptibility and pathogenicity of Aeromonas hydrophila isolated from red hybrid tilapia ( Oreochromis niloticus× Oreochromis mossambicus) in Malaysia |
| title_full | Antibiotic susceptibility and pathogenicity of Aeromonas hydrophila isolated from red hybrid tilapia ( Oreochromis niloticus× Oreochromis mossambicus) in Malaysia |
| title_fullStr | Antibiotic susceptibility and pathogenicity of Aeromonas hydrophila isolated from red hybrid tilapia ( Oreochromis niloticus× Oreochromis mossambicus) in Malaysia |
| title_full_unstemmed | Antibiotic susceptibility and pathogenicity of Aeromonas hydrophila isolated from red hybrid tilapia ( Oreochromis niloticus× Oreochromis mossambicus) in Malaysia |
| title_short | Antibiotic susceptibility and pathogenicity of Aeromonas hydrophila isolated from red hybrid tilapia ( Oreochromis niloticus× Oreochromis mossambicus) in Malaysia |
| title_sort | antibiotic susceptibility and pathogenicity of aeromonas hydrophila isolated from red hybrid tilapia oreochromis niloticus oreochromis mossambicus in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/87890/1/ABSTRACT.pdf |
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