Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica
Bioremediation has been proposed as a means of dealing with oil spills on the continent. However, the introduction of non-native organisms, including microbes, even for this purpose would appear to breach the terms of the Environmental Protocol to the Antarctic Treaty. This study therefore aimed to...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88030/1/ABSTRACT.pdf |
| _version_ | 1825952440426430464 |
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| author | Abdulrasheed, Mansur Zakaria, Nur Nadhirah Ahmad Roslee, Ahmad Fareez Shukor, Mohd Yunus Zulkharnain, Azham Napis, Suhaimi Convey, Peter Gonzalez-Rocha, Gerardo Ahmad, Siti Aqlima |
| author_facet | Abdulrasheed, Mansur Zakaria, Nur Nadhirah Ahmad Roslee, Ahmad Fareez Shukor, Mohd Yunus Zulkharnain, Azham Napis, Suhaimi Convey, Peter Gonzalez-Rocha, Gerardo Ahmad, Siti Aqlima |
| author_sort | Abdulrasheed, Mansur |
| collection | UPM |
| description | Bioremediation has been proposed as a means of dealing with oil spills on the continent. However, the introduction of non-native organisms, including microbes, even for this purpose would appear to breach the terms of the Environmental Protocol to the Antarctic Treaty. This study therefore aimed to optimize the growth conditions and diesel degradation activity of the Antarctic native bacteria Arthrobacter spp. strains AQ5-05 and AQ5-06 through the application of a one-factor-at-a-time (OFAT) approach. Both strains were psychrotolerant, with the optimum temperature supporting diesel degradation being 10–15°C. Both strains were also screened for biosurfactant production and biofilm formation. Their diesel degradation potential was assessed using Bushnell–Haas medium supplemented with 0.5% (v/v) diesel as the sole carbon source and determined using both gravimetric and gas chromatography and mass spectrophotometry analysis. Strain AQ5-06 achieved 37.5% diesel degradation, while strain AQ5-05 achieved 34.5% diesel degradation. Both strains produced biosurfactants and showed high biofilm adherence. Strains AQ5-05 and AQ5-06 showed high cellular hydrophobicity rates of 73.0% and 81.5%, respectively, in hexadecane, with somewhat lower values of 60.5% and 70.5%, respectively, in tetrahexadecane. Optimized conditions identified via OFAT increased diesel degradation to 41.0% and 47.5% for strains AQ5-05 and AQ5-06, respectively. Both strains also demonstrated the ability to degrade diesel in the presence of heavy metal co-pollutants. This study therefore confirms the potential use of these cold-tolerant bacterial strains in the biodegradation of diesel-polluted Antarctic soils at low environmental temperatures. |
| first_indexed | 2024-03-06T10:44:40Z |
| format | Article |
| id | upm.eprints-88030 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:44:40Z |
| publishDate | 2020 |
| publisher | Cambridge University Press |
| record_format | dspace |
| spelling | upm.eprints-880302022-05-24T04:56:59Z http://psasir.upm.edu.my/id/eprint/88030/ Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica Abdulrasheed, Mansur Zakaria, Nur Nadhirah Ahmad Roslee, Ahmad Fareez Shukor, Mohd Yunus Zulkharnain, Azham Napis, Suhaimi Convey, Peter Gonzalez-Rocha, Gerardo Ahmad, Siti Aqlima Bioremediation has been proposed as a means of dealing with oil spills on the continent. However, the introduction of non-native organisms, including microbes, even for this purpose would appear to breach the terms of the Environmental Protocol to the Antarctic Treaty. This study therefore aimed to optimize the growth conditions and diesel degradation activity of the Antarctic native bacteria Arthrobacter spp. strains AQ5-05 and AQ5-06 through the application of a one-factor-at-a-time (OFAT) approach. Both strains were psychrotolerant, with the optimum temperature supporting diesel degradation being 10–15°C. Both strains were also screened for biosurfactant production and biofilm formation. Their diesel degradation potential was assessed using Bushnell–Haas medium supplemented with 0.5% (v/v) diesel as the sole carbon source and determined using both gravimetric and gas chromatography and mass spectrophotometry analysis. Strain AQ5-06 achieved 37.5% diesel degradation, while strain AQ5-05 achieved 34.5% diesel degradation. Both strains produced biosurfactants and showed high biofilm adherence. Strains AQ5-05 and AQ5-06 showed high cellular hydrophobicity rates of 73.0% and 81.5%, respectively, in hexadecane, with somewhat lower values of 60.5% and 70.5%, respectively, in tetrahexadecane. Optimized conditions identified via OFAT increased diesel degradation to 41.0% and 47.5% for strains AQ5-05 and AQ5-06, respectively. Both strains also demonstrated the ability to degrade diesel in the presence of heavy metal co-pollutants. This study therefore confirms the potential use of these cold-tolerant bacterial strains in the biodegradation of diesel-polluted Antarctic soils at low environmental temperatures. Cambridge University Press 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88030/1/ABSTRACT.pdf Abdulrasheed, Mansur and Zakaria, Nur Nadhirah and Ahmad Roslee, Ahmad Fareez and Shukor, Mohd Yunus and Zulkharnain, Azham and Napis, Suhaimi and Convey, Peter and Gonzalez-Rocha, Gerardo and Ahmad, Siti Aqlima (2020) Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica. Antarctic Science, 32 (5). pp. 341-353. ISSN 0954-1020; ESSN: 1365-2079 https://www.cambridge.org/core/journals/antarctic-science/article/biodegradation-of-diesel-oil-by-coldadapted-bacterial-strains-of-arthrobacter-spp-from-antarctica/93A5CD6F8BD75C505B7A17D9662823DD 10.1017/S0954102020000206 |
| spellingShingle | Abdulrasheed, Mansur Zakaria, Nur Nadhirah Ahmad Roslee, Ahmad Fareez Shukor, Mohd Yunus Zulkharnain, Azham Napis, Suhaimi Convey, Peter Gonzalez-Rocha, Gerardo Ahmad, Siti Aqlima Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica |
| title | Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica |
| title_full | Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica |
| title_fullStr | Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica |
| title_full_unstemmed | Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica |
| title_short | Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica |
| title_sort | biodegradation of diesel oil by cold adapted bacterial strains of arthrobacter spp from antarctica |
| url | http://psasir.upm.edu.my/id/eprint/88030/1/ABSTRACT.pdf |
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