Biological activities of selected plants and detection of bioactive compounds from Ardisia elliptica using UHPLC-Q-Exactive Orbitrap mass spectrometry
Plants and plant-based products have been used for a long time for medicinal purposes. This study aimed to determine the antioxidant and anti-α-glucosidase activities of eight selected underutilized plants in Malaysia: Leucaena leucocephala, Muntingia calabura, Spondias dulcis, Annona squamosa, Ardi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88037/1/ABSTRACT.pdf |
| _version_ | 1825952441613418496 |
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| author | Wong, Pei Lou Fauzi, Nurul Azila Mohamed Yunus, Siti Norhamimah Abdul Hamid, Nur Ashikin Abd Ghafar, Siti Zulaikha Azizan, Awanis Zolkeflee, Nur Khaleeda Zulaikha Abas, Faridah |
| author_facet | Wong, Pei Lou Fauzi, Nurul Azila Mohamed Yunus, Siti Norhamimah Abdul Hamid, Nur Ashikin Abd Ghafar, Siti Zulaikha Azizan, Awanis Zolkeflee, Nur Khaleeda Zulaikha Abas, Faridah |
| author_sort | Wong, Pei Lou |
| collection | UPM |
| description | Plants and plant-based products have been used for a long time for medicinal purposes. This study aimed to determine the antioxidant and anti-α-glucosidase activities of eight selected underutilized plants in Malaysia: Leucaena leucocephala, Muntingia calabura, Spondias dulcis, Annona squamosa, Ardisia elliptica, Cynometra cauliflora, Ficus auriculata, and Averrhoa bilimbi. This study showed that the 70% ethanolic extract of all plants exhibited total phenolic content (TPC) ranging from 51 to 344 mg gallic acid equivalent (GAE)/g dry weight. A. elliptica showed strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) scavenging activities, with half maximal inhibitory concentration (IC50) values of 2.17 and 49.43 μg/mL, respectively. Most of the tested plant extracts showed higher inhibition of α-glucosidase enzyme activity than the standard, quercetin, particularly A. elliptica, F. auriculata, and M. calabura extracts with IC50 values of 0.29, 0.36, and 0.51 μg/mL, respectively. A total of 62 metabolites including flavonoids, triterpenoids, benzoquinones, and fatty acids were tentatively identified in the most active plant, i.e., A. elliptica leaf extract, by using ultra-high-performance liquid chromatography (UHPLC)–electrospray ionization (ESI) Orbitrap MS. This study suggests a potential natural source of antioxidant and α-glucosidase inhibitors from A. elliptica. |
| first_indexed | 2024-03-06T10:44:41Z |
| format | Article |
| id | upm.eprints-88037 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:44:41Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-880372022-05-24T04:52:41Z http://psasir.upm.edu.my/id/eprint/88037/ Biological activities of selected plants and detection of bioactive compounds from Ardisia elliptica using UHPLC-Q-Exactive Orbitrap mass spectrometry Wong, Pei Lou Fauzi, Nurul Azila Mohamed Yunus, Siti Norhamimah Abdul Hamid, Nur Ashikin Abd Ghafar, Siti Zulaikha Azizan, Awanis Zolkeflee, Nur Khaleeda Zulaikha Abas, Faridah Plants and plant-based products have been used for a long time for medicinal purposes. This study aimed to determine the antioxidant and anti-α-glucosidase activities of eight selected underutilized plants in Malaysia: Leucaena leucocephala, Muntingia calabura, Spondias dulcis, Annona squamosa, Ardisia elliptica, Cynometra cauliflora, Ficus auriculata, and Averrhoa bilimbi. This study showed that the 70% ethanolic extract of all plants exhibited total phenolic content (TPC) ranging from 51 to 344 mg gallic acid equivalent (GAE)/g dry weight. A. elliptica showed strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) scavenging activities, with half maximal inhibitory concentration (IC50) values of 2.17 and 49.43 μg/mL, respectively. Most of the tested plant extracts showed higher inhibition of α-glucosidase enzyme activity than the standard, quercetin, particularly A. elliptica, F. auriculata, and M. calabura extracts with IC50 values of 0.29, 0.36, and 0.51 μg/mL, respectively. A total of 62 metabolites including flavonoids, triterpenoids, benzoquinones, and fatty acids were tentatively identified in the most active plant, i.e., A. elliptica leaf extract, by using ultra-high-performance liquid chromatography (UHPLC)–electrospray ionization (ESI) Orbitrap MS. This study suggests a potential natural source of antioxidant and α-glucosidase inhibitors from A. elliptica. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88037/1/ABSTRACT.pdf Wong, Pei Lou and Fauzi, Nurul Azila and Mohamed Yunus, Siti Norhamimah and Abdul Hamid, Nur Ashikin and Abd Ghafar, Siti Zulaikha and Azizan, Awanis and Zolkeflee, Nur Khaleeda Zulaikha and Abas, Faridah (2020) Biological activities of selected plants and detection of bioactive compounds from Ardisia elliptica using UHPLC-Q-Exactive Orbitrap mass spectrometry. Molecules, 25 (13). pp. 1-19. ISSN 1420-3049 https://www.mdpi.com/1420-3049/25/13/3067 10.3390/molecules25133067 |
| spellingShingle | Wong, Pei Lou Fauzi, Nurul Azila Mohamed Yunus, Siti Norhamimah Abdul Hamid, Nur Ashikin Abd Ghafar, Siti Zulaikha Azizan, Awanis Zolkeflee, Nur Khaleeda Zulaikha Abas, Faridah Biological activities of selected plants and detection of bioactive compounds from Ardisia elliptica using UHPLC-Q-Exactive Orbitrap mass spectrometry |
| title | Biological activities of selected plants and detection of bioactive compounds from Ardisia elliptica using UHPLC-Q-Exactive Orbitrap mass spectrometry |
| title_full | Biological activities of selected plants and detection of bioactive compounds from Ardisia elliptica using UHPLC-Q-Exactive Orbitrap mass spectrometry |
| title_fullStr | Biological activities of selected plants and detection of bioactive compounds from Ardisia elliptica using UHPLC-Q-Exactive Orbitrap mass spectrometry |
| title_full_unstemmed | Biological activities of selected plants and detection of bioactive compounds from Ardisia elliptica using UHPLC-Q-Exactive Orbitrap mass spectrometry |
| title_short | Biological activities of selected plants and detection of bioactive compounds from Ardisia elliptica using UHPLC-Q-Exactive Orbitrap mass spectrometry |
| title_sort | biological activities of selected plants and detection of bioactive compounds from ardisia elliptica using uhplc q exactive orbitrap mass spectrometry |
| url | http://psasir.upm.edu.my/id/eprint/88037/1/ABSTRACT.pdf |
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