Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites
Gray mold disease is caused by Botrytis cinerea and it is the most significant and devastating disease affecting tomato cultivation worldwide. This disease was recently reported infecting tomato fruits in Cameroon Highland, Pahang. Biocontrol agents have been proven to be effective towards many phyt...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society of Applied Biology
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88262/1/ABSTRACT.pdf |
| _version_ | 1796981949804314624 |
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| author | Hashim, Arafat Ismail, Siti Izera Alsultan, Wael M. K. Abu Seman, Idris Rashed, Osamah Zaid Ali Ahmad, Khairulmazmi |
| author_facet | Hashim, Arafat Ismail, Siti Izera Alsultan, Wael M. K. Abu Seman, Idris Rashed, Osamah Zaid Ali Ahmad, Khairulmazmi |
| author_sort | Hashim, Arafat |
| collection | UPM |
| description | Gray mold disease is caused by Botrytis cinerea and it is the most significant and devastating disease affecting tomato cultivation worldwide. This disease was recently reported infecting tomato fruits in Cameroon Highland, Pahang. Biocontrol agents have been proven to be effective towards many phytopathogens. In this study, 43 bacterial isolates were screened against B. cinerea in-vitro. Five isolates with more than 65% inhibition of radial growth (PIRG) were selected for the subsequent experiments. Findings in dual culture assay revealed that BM11 and BC4 were the most effective biocontrol agent, with PIRG of 82.5% and 71.8%, respectively. BM11 and BC4 isolates were identified using the 16S rDNA gene. The identification results found that BM11 and BC4 were Pseudomonas protegens and Brevibacterium casei, respectively. Ten major bioactive compounds were successfully identified by Gas chromatography-mass spectrometry (GC-MS) from the culture filtrate of both biological control agent (BCA) isolates. These bioactive compounds may responsible for antifungal activity. In vivo study revealed that culture filtrate extract manages to suppress disease lesions on the treated tomato fruit from 0.86 -1.03 cM, 30 days after inoculation. Based on overall findings suggested that P. protegens and B. casei were the promising BCA in controlling gray mold disease of tomato fruit. |
| first_indexed | 2024-03-06T10:45:09Z |
| format | Article |
| id | upm.eprints-88262 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:45:09Z |
| publishDate | 2020 |
| publisher | Malaysian Society of Applied Biology |
| record_format | dspace |
| spelling | upm.eprints-882622022-11-24T04:35:06Z http://psasir.upm.edu.my/id/eprint/88262/ Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites Hashim, Arafat Ismail, Siti Izera Alsultan, Wael M. K. Abu Seman, Idris Rashed, Osamah Zaid Ali Ahmad, Khairulmazmi Gray mold disease is caused by Botrytis cinerea and it is the most significant and devastating disease affecting tomato cultivation worldwide. This disease was recently reported infecting tomato fruits in Cameroon Highland, Pahang. Biocontrol agents have been proven to be effective towards many phytopathogens. In this study, 43 bacterial isolates were screened against B. cinerea in-vitro. Five isolates with more than 65% inhibition of radial growth (PIRG) were selected for the subsequent experiments. Findings in dual culture assay revealed that BM11 and BC4 were the most effective biocontrol agent, with PIRG of 82.5% and 71.8%, respectively. BM11 and BC4 isolates were identified using the 16S rDNA gene. The identification results found that BM11 and BC4 were Pseudomonas protegens and Brevibacterium casei, respectively. Ten major bioactive compounds were successfully identified by Gas chromatography-mass spectrometry (GC-MS) from the culture filtrate of both biological control agent (BCA) isolates. These bioactive compounds may responsible for antifungal activity. In vivo study revealed that culture filtrate extract manages to suppress disease lesions on the treated tomato fruit from 0.86 -1.03 cM, 30 days after inoculation. Based on overall findings suggested that P. protegens and B. casei were the promising BCA in controlling gray mold disease of tomato fruit. Malaysian Society of Applied Biology 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88262/1/ABSTRACT.pdf Hashim, Arafat and Ismail, Siti Izera and Alsultan, Wael M. K. and Abu Seman, Idris and Rashed, Osamah Zaid Ali and Ahmad, Khairulmazmi (2020) Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites. Malaysian Applied Biology, 49 (5). 89 - 97. ISSN 2462-151X https://jms.mabjournal.com/index.php/mab/article/view/1641 10.55230/mabjournal.v49i5.1641 |
| spellingShingle | Hashim, Arafat Ismail, Siti Izera Alsultan, Wael M. K. Abu Seman, Idris Rashed, Osamah Zaid Ali Ahmad, Khairulmazmi Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites |
| title | Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites |
| title_full | Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites |
| title_fullStr | Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites |
| title_full_unstemmed | Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites |
| title_short | Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites |
| title_sort | control of gray mold disease of tomato caused by botrytis cinerea using bacterial secondary metabolites |
| url | http://psasir.upm.edu.my/id/eprint/88262/1/ABSTRACT.pdf |
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