Cross‑amplifcation of microsatellite markers across agarwood‑producing species of the Aquilarieae tribe (Thymelaeaceae)
Tree species in the Aquilarieae tribe of the Thymelaeaceae family produce agarwood, a natural product highly valued for its fragrance, but the species are under threat due to indiscriminate harvesting. For conservation of these species, molecular techniques such as DNA profiling have been used. In t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88306/1/ABSTRACT.pdf |
| _version_ | 1796981955003154432 |
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| author | Pern, Yu Cong Lee, Shiou Yih Wei, Lun Ng Mohamed, Rozi |
| author_facet | Pern, Yu Cong Lee, Shiou Yih Wei, Lun Ng Mohamed, Rozi |
| author_sort | Pern, Yu Cong |
| collection | UPM |
| description | Tree species in the Aquilarieae tribe of the Thymelaeaceae family produce agarwood, a natural product highly valued for its fragrance, but the species are under threat due to indiscriminate harvesting. For conservation of these species, molecular techniques such as DNA profiling have been used. In this study, we assessed cross-amplification of microsatellite markers, initially developed for three Aquilaria species (A. crassna, A. malaccensis, and A. sinensis), on ten other agarwood-producing species, including members of Aquilaria (A. beccariana, A. hirta, A. microcarpa, A. rostrata, A. rugosa, A. subintegra, and A. yunnanensis) and Gyrinops (G. caudata, G. versteegii, and G. walla), both from the Aquilarieae tribe. Primers for 18 out of the 30 microsatellite markers successfully amplified bands of expected sizes in 1 sample each of at least 10 species. These were further used to genotype 74 individuals representing all the 13 studied species, yielding 13 cross-amplifiable markers, of which only 1 being polymorphic across all species. At each locus, the number of alleles ranged from 7 to 23, indicating a rather high variability. Four markers had relatively high species discrimination power. Our results demonstrated that genetic fingerprinting can be an effective tool in helping to manage agarwood genetic resources by potentially supporting the chain-of-custody of agarwood and its products in the market. |
| first_indexed | 2024-03-06T10:45:14Z |
| format | Article |
| id | upm.eprints-88306 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:45:14Z |
| publishDate | 2020 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-883062022-11-24T01:59:03Z http://psasir.upm.edu.my/id/eprint/88306/ Cross‑amplifcation of microsatellite markers across agarwood‑producing species of the Aquilarieae tribe (Thymelaeaceae) Pern, Yu Cong Lee, Shiou Yih Wei, Lun Ng Mohamed, Rozi Tree species in the Aquilarieae tribe of the Thymelaeaceae family produce agarwood, a natural product highly valued for its fragrance, but the species are under threat due to indiscriminate harvesting. For conservation of these species, molecular techniques such as DNA profiling have been used. In this study, we assessed cross-amplification of microsatellite markers, initially developed for three Aquilaria species (A. crassna, A. malaccensis, and A. sinensis), on ten other agarwood-producing species, including members of Aquilaria (A. beccariana, A. hirta, A. microcarpa, A. rostrata, A. rugosa, A. subintegra, and A. yunnanensis) and Gyrinops (G. caudata, G. versteegii, and G. walla), both from the Aquilarieae tribe. Primers for 18 out of the 30 microsatellite markers successfully amplified bands of expected sizes in 1 sample each of at least 10 species. These were further used to genotype 74 individuals representing all the 13 studied species, yielding 13 cross-amplifiable markers, of which only 1 being polymorphic across all species. At each locus, the number of alleles ranged from 7 to 23, indicating a rather high variability. Four markers had relatively high species discrimination power. Our results demonstrated that genetic fingerprinting can be an effective tool in helping to manage agarwood genetic resources by potentially supporting the chain-of-custody of agarwood and its products in the market. Springer 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88306/1/ABSTRACT.pdf Pern, Yu Cong and Lee, Shiou Yih and Wei, Lun Ng and Mohamed, Rozi (2020) Cross‑amplifcation of microsatellite markers across agarwood‑producing species of the Aquilarieae tribe (Thymelaeaceae). 3 Biotech, 10 (3). art. no. 103. pp. 1-9. ISSN 2190-572X; ESSN: 2190-5738 https://link.springer.com/article/10.1007/s13205-020-2072-2 10.1007/s13205-020-2072-2 |
| spellingShingle | Pern, Yu Cong Lee, Shiou Yih Wei, Lun Ng Mohamed, Rozi Cross‑amplifcation of microsatellite markers across agarwood‑producing species of the Aquilarieae tribe (Thymelaeaceae) |
| title | Cross‑amplifcation of microsatellite markers across agarwood‑producing species of the Aquilarieae tribe (Thymelaeaceae) |
| title_full | Cross‑amplifcation of microsatellite markers across agarwood‑producing species of the Aquilarieae tribe (Thymelaeaceae) |
| title_fullStr | Cross‑amplifcation of microsatellite markers across agarwood‑producing species of the Aquilarieae tribe (Thymelaeaceae) |
| title_full_unstemmed | Cross‑amplifcation of microsatellite markers across agarwood‑producing species of the Aquilarieae tribe (Thymelaeaceae) |
| title_short | Cross‑amplifcation of microsatellite markers across agarwood‑producing species of the Aquilarieae tribe (Thymelaeaceae) |
| title_sort | cross amplifcation of microsatellite markers across agarwood producing species of the aquilarieae tribe thymelaeaceae |
| url | http://psasir.upm.edu.my/id/eprint/88306/1/ABSTRACT.pdf |
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