Detection of Leptospira kmetyi at recreational areas in Peninsular Malaysia
Humans can be infected by pathogenic Leptospira through contact with infected animals or contaminated environments. Recreational exposure has been associated with human leptospirosis; however, there is a lack of information on the distribution of Leptospira spp. in recreational areas. Thus, we condu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88385/1/ABSTRACT.pdf |
| _version_ | 1825936510363369472 |
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| author | Mohamad Zaki, Aznida Hod, Rozita Shamsusah, Nadia Aqilla Md Isa, Zaleha Bejo, Siti Khairani Agustar, Hani Kartini |
| author_facet | Mohamad Zaki, Aznida Hod, Rozita Shamsusah, Nadia Aqilla Md Isa, Zaleha Bejo, Siti Khairani Agustar, Hani Kartini |
| author_sort | Mohamad Zaki, Aznida |
| collection | UPM |
| description | Humans can be infected by pathogenic Leptospira through contact with infected animals or contaminated environments. Recreational exposure has been associated with human leptospirosis; however, there is a lack of information on the distribution of Leptospira spp. in recreational areas. Thus, we conducted this study to detect and describe the distributions of Leptospira spp. and to determine the prevalence of pathogenic leptospires at recreational areas in Peninsular Malaysia. Soil and water samples were randomly collected from 33 recreational areas in Peninsular Malaysia from December 2018 to April 2019. Each culture was examined under dark-field microscopy prior to genus confirmation by polymerase chain reaction using primers for the 16S rRNA gene. A total of 390 water and soil samples were collected, and 131 cultures were positive for Leptospira under dark-field microscope examination. Leptospira was identified in most of the recreational areas sampled, and at least one pathogenic Leptospira species was isolated from 17 recreational areas. The prevalence of saprophytic, intermediate, and pathogenic Leptospira spp. was 19.7%, 5.6%, and 8.2%, respectively. The dominant pathogenic species found in the samples was Leptospira kmetyi. This study provides important data on the distribution and prevalence of Leptospira spp. from soil and water, as well as the dominant pathogenic species, at recreational areas in Peninsular Malaysia. Therefore, preventive measures should be taken to ensure the safety of visitors. |
| first_indexed | 2024-03-06T10:45:23Z |
| format | Article |
| id | upm.eprints-88385 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:45:23Z |
| publishDate | 2020 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-883852021-12-29T01:01:37Z http://psasir.upm.edu.my/id/eprint/88385/ Detection of Leptospira kmetyi at recreational areas in Peninsular Malaysia Mohamad Zaki, Aznida Hod, Rozita Shamsusah, Nadia Aqilla Md Isa, Zaleha Bejo, Siti Khairani Agustar, Hani Kartini Humans can be infected by pathogenic Leptospira through contact with infected animals or contaminated environments. Recreational exposure has been associated with human leptospirosis; however, there is a lack of information on the distribution of Leptospira spp. in recreational areas. Thus, we conducted this study to detect and describe the distributions of Leptospira spp. and to determine the prevalence of pathogenic leptospires at recreational areas in Peninsular Malaysia. Soil and water samples were randomly collected from 33 recreational areas in Peninsular Malaysia from December 2018 to April 2019. Each culture was examined under dark-field microscopy prior to genus confirmation by polymerase chain reaction using primers for the 16S rRNA gene. A total of 390 water and soil samples were collected, and 131 cultures were positive for Leptospira under dark-field microscope examination. Leptospira was identified in most of the recreational areas sampled, and at least one pathogenic Leptospira species was isolated from 17 recreational areas. The prevalence of saprophytic, intermediate, and pathogenic Leptospira spp. was 19.7%, 5.6%, and 8.2%, respectively. The dominant pathogenic species found in the samples was Leptospira kmetyi. This study provides important data on the distribution and prevalence of Leptospira spp. from soil and water, as well as the dominant pathogenic species, at recreational areas in Peninsular Malaysia. Therefore, preventive measures should be taken to ensure the safety of visitors. Springer 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88385/1/ABSTRACT.pdf Mohamad Zaki, Aznida and Hod, Rozita and Shamsusah, Nadia Aqilla and Md Isa, Zaleha and Bejo, Siti Khairani and Agustar, Hani Kartini (2020) Detection of Leptospira kmetyi at recreational areas in Peninsular Malaysia. Environmental Monitoring and Assessment, 192. art. no. 703. pp. 1-12. ISSN 0167-6369; ESSN: 1573-2959 https://link.springer.com/article/10.1007%2Fs10661-020-08639-x 10.1007/s10661-020-08639-x |
| spellingShingle | Mohamad Zaki, Aznida Hod, Rozita Shamsusah, Nadia Aqilla Md Isa, Zaleha Bejo, Siti Khairani Agustar, Hani Kartini Detection of Leptospira kmetyi at recreational areas in Peninsular Malaysia |
| title | Detection of Leptospira kmetyi at recreational areas in Peninsular Malaysia |
| title_full | Detection of Leptospira kmetyi at recreational areas in Peninsular Malaysia |
| title_fullStr | Detection of Leptospira kmetyi at recreational areas in Peninsular Malaysia |
| title_full_unstemmed | Detection of Leptospira kmetyi at recreational areas in Peninsular Malaysia |
| title_short | Detection of Leptospira kmetyi at recreational areas in Peninsular Malaysia |
| title_sort | detection of leptospira kmetyi at recreational areas in peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/88385/1/ABSTRACT.pdf |
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