Detection of phenol by incorporation of gold modified-enzyme based graphene oxide thin film with surface plasmon resonance technique
In this study, the incorporation between gold modified-tyrosinase (Tyr) enzyme based graphene oxide (GO) thin film with surface plasmon resonance (SPR) technique has been developed for the detection of phenol. SPR signal for the thin film contacted with phenol solution was monitored using SPR techni...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Optical Society of America
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88387/1/ABSTRACT.pdf |
| _version_ | 1825936510561550336 |
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| author | Hashim, Hazwani Suhaila Yap, Wing Fen Sheh Omar, Nur Alia Abdullah, Jaafar Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Saleviter, Silvan |
| author_facet | Hashim, Hazwani Suhaila Yap, Wing Fen Sheh Omar, Nur Alia Abdullah, Jaafar Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Saleviter, Silvan |
| author_sort | Hashim, Hazwani Suhaila |
| collection | UPM |
| description | In this study, the incorporation between gold modified-tyrosinase (Tyr) enzyme based graphene oxide (GO) thin film with surface plasmon resonance (SPR) technique has been developed for the detection of phenol. SPR signal for the thin film contacted with phenol solution was monitored using SPR technique. From the SPR curve, sensitivity, full width at half maximum (FWHM), detection accuracy (DA) and signal-to-noise ratio (SNR) have been analyzed. The sensor produces a linear response for phenol up to 100 µM with sensitivity of 0.00193° µM-1. Next, it can be observed that deionized water has the lowest FWHM, with a value of 1.87° and also the highest value of DA. Besides, the SNR of the SPR signal was proportional to the phenol concentrations. Furthermore, the surface morphology of the modified thin film after exposed with phenol solution observed using atomic force microscopy showed a lot of sharp peaks compared to the image before in contact with phenol proved the interaction between the thin film and phenol. |
| first_indexed | 2024-03-06T10:45:23Z |
| format | Article |
| id | upm.eprints-88387 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:45:23Z |
| publishDate | 2020 |
| publisher | Optical Society of America |
| record_format | dspace |
| spelling | upm.eprints-883872021-12-29T01:05:34Z http://psasir.upm.edu.my/id/eprint/88387/ Detection of phenol by incorporation of gold modified-enzyme based graphene oxide thin film with surface plasmon resonance technique Hashim, Hazwani Suhaila Yap, Wing Fen Sheh Omar, Nur Alia Abdullah, Jaafar Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Saleviter, Silvan In this study, the incorporation between gold modified-tyrosinase (Tyr) enzyme based graphene oxide (GO) thin film with surface plasmon resonance (SPR) technique has been developed for the detection of phenol. SPR signal for the thin film contacted with phenol solution was monitored using SPR technique. From the SPR curve, sensitivity, full width at half maximum (FWHM), detection accuracy (DA) and signal-to-noise ratio (SNR) have been analyzed. The sensor produces a linear response for phenol up to 100 µM with sensitivity of 0.00193° µM-1. Next, it can be observed that deionized water has the lowest FWHM, with a value of 1.87° and also the highest value of DA. Besides, the SNR of the SPR signal was proportional to the phenol concentrations. Furthermore, the surface morphology of the modified thin film after exposed with phenol solution observed using atomic force microscopy showed a lot of sharp peaks compared to the image before in contact with phenol proved the interaction between the thin film and phenol. Optical Society of America 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88387/1/ABSTRACT.pdf Hashim, Hazwani Suhaila and Yap, Wing Fen and Sheh Omar, Nur Alia and Abdullah, Jaafar and Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim and Saleviter, Silvan (2020) Detection of phenol by incorporation of gold modified-enzyme based graphene oxide thin film with surface plasmon resonance technique. Optics Express, 28 (7). pp. 9738-9752. ISSN 1094-4087 https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-28-7-9738&id=429346 10.1364/OE.387027 |
| spellingShingle | Hashim, Hazwani Suhaila Yap, Wing Fen Sheh Omar, Nur Alia Abdullah, Jaafar Mohd Daniyal, Wan Mohd Ebtisyam Mustaqim Saleviter, Silvan Detection of phenol by incorporation of gold modified-enzyme based graphene oxide thin film with surface plasmon resonance technique |
| title | Detection of phenol by incorporation of gold modified-enzyme based graphene oxide thin film with surface plasmon resonance technique |
| title_full | Detection of phenol by incorporation of gold modified-enzyme based graphene oxide thin film with surface plasmon resonance technique |
| title_fullStr | Detection of phenol by incorporation of gold modified-enzyme based graphene oxide thin film with surface plasmon resonance technique |
| title_full_unstemmed | Detection of phenol by incorporation of gold modified-enzyme based graphene oxide thin film with surface plasmon resonance technique |
| title_short | Detection of phenol by incorporation of gold modified-enzyme based graphene oxide thin film with surface plasmon resonance technique |
| title_sort | detection of phenol by incorporation of gold modified enzyme based graphene oxide thin film with surface plasmon resonance technique |
| url | http://psasir.upm.edu.my/id/eprint/88387/1/ABSTRACT.pdf |
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